QTL Mapping for Seed Germination Response to Drought Stress in Brassica napus

Gad, Mahmoud; Chao, Hongbo; Li, Huaixin; Zhao, Weiguo; Lu, Guangyuan; Li, Maoteng

doi:10.3389/fpls.2020.629970

Cited by 21 publications

(15 citation statements)

References 46 publications

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“…Seed germination is not only the beginning of plant growth and development, but is also a key link that determines the quality of seedling growth [ 13 ]. However, seed germination is susceptible to many factors, such as drought [ 14 ], light [ 15 ], temperature [ 16 ], saline–alkaline [ 17 ], and heavy metals [ 18 ]. Unsuitable environmental conditions can readily compromise seed germination rate, leading to weak seedling growth.…”

Section: Introductionmentioning

confidence: 99%

Exogenous SA Affects Rice Seed Germination under Salt Stress by Regulating Na+/K+ Balance and Endogenous GAs and ABA Homeostasis

Liu

Hou

et al. 2022

IJMS

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Salinity reduces agricultural productivity majorly by inhibiting seed germination. Exogenous salicylic acid (SA) can prevent the harm caused to rice by salinity, but the mechanisms by which it promotes rice seed germination under salt stress are unclear. In this study, the inhibition of germination in salt-sensitive Nipponbare under salt stress was greater than that in salt-tolerant Huaidao 5. Treatment with exogenous SA significantly improved germination of Nipponbare, but had little effect on Huaidao 5. The effects of exogenous SA on ion balance, metabolism of reactive oxygen species (ROS), hormone homeostasis, starch hydrolysis, and other physiological processes involved in seed germination of rice under salt stress were investigated. Under salt stress, Na+ content and the Na+/K+ ratio in rice seeds increased sharply. Seeds were subjected to ion pressure, which led to massive accumulation of H2O2, O2−, and malonaldehyde (MDA); imbalanced endogenous hormone homeostasis; decreased gibberellic acid (GA1 and GA4) content; increased abscisic acid (ABA) content; inhibition of α-amylase (EC 3.2.1.1) activity; and slowed starch hydrolysis rate, all which eventually led to the inhibition of the germination of rice seeds. Exogenous SA could effectively enhance the expression of OsHKT1;1, OsHKT1;5, OsHKT2;1 and OsSOS1 to reduce the absorption of Na+ by seeds; reduce the Na+/K+ ratio; improve the activities of SOD, POD, and CAT; reduce the accumulation of H2O2, O2−, and MDA; enhance the expression of the GA biosynthetic genes OsGA20ox1 and OsGA3ox2; inhibit the expression of the ABA biosynthetic gene OsNCED5; increase GA1 and GA4 content; reduce ABA content; improve α-amylase activity, and increase the content of soluble sugars. In summary, exogenous SA can alleviate ion toxicity by reducing Na+ content, thereby helping to maintain ROS and hormone homeostasis, promote starch hydrolysis, and provide sufficient energy for seed germination, all of which ultimately improves rice seed germination under salt stress. This study presents a feasible means for improving the germination of direct-seeded rice in saline soil.

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Section: Introductionmentioning

confidence: 99%

Exogenous SA Affects Rice Seed Germination under Salt Stress by Regulating Na+/K+ Balance and Endogenous GAs and ABA Homeostasis

Liu

Hou

et al. 2022

IJMS

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“…Seed germination is related to vegetative growth and reproductive growth of plants in the later stage (Luan et al, 2014), while seed germination and seedling growth will directly affect the distribution and diversity of plants (Wulff, 1986;Souza and Fagundes, 2014). And in recent years, with global warming, the degree of harm to drought and salinity has gradually increased (Trenberth et al, 2014;Gad et al, 2021;Zhou et al, 2022). Therefore, it is of great theoretical and practical significance to study the germination characteristics of plant seeds under salt and drought stress to comprehensively consider the salt and drought resistance of plants and the distribution and diversity of plant populations, especially in arid and semi-arid ecosystems.…”

Section: Introductionmentioning

confidence: 99%

Effects of drought and salt stress on seed germination of ephemeral plants in desert of northwest China

Liu

Chen

et al. 2022

Front. Ecol. Evol.

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Seed germination is the most sensitive stage of plant life history. Studying its response to drought and salinity can analysis the response and adaptation characteristics of desert plants to the environment. In this experiment, the seeds of four common desert ephemeral plants in Xinjiang (Ixiolirion tataricum, Nepeta micrantha, Lepidium apetalum, and Plantago minuta) were used as materials. To study the germination characteristics of seeds under drought, we used salt stress, and coupled salt-drought stress under treatments and explored the germination recovery ability of rehydrated seeds after salt and drought stress treatments. The results showed that: (1) Under salt stress, the germination ability of four plant seeds was inhibited in different degrees. Overall, the degree of inhibition was proportional to the concentration of NaCl solution. (2) Drought stress slowed the seed germination process, and the greater the degree of stress, the more pronounced the slowdown; PEG treatment showed no significant effect on the germination of the four seeds at low concentrations and significant inhibition at medium and high concentrations. (3) The coupled salt-drought treatment significantly alleviated the stress effect of one factor and improved the germination characteristics of seeds. (4) Seeds that did not germinate under different concentrations of salt stress, drought stress, and coupled stresses rapidly recover germination when the stress was relieved or lifted. Ephemeral plants are sensitive to environmental changes, and this study aims to provide a reference basis for vegetation restoration and ecological rehabilitation in arid and semi-arid areas.

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“…In Arabidopsis, Delay of Germination 1 and Reduced Dormancy 5 genes have been identified by gene localization, regulating the level of seed dormancy via the gibberellin (GA) signaling pathway. The genetic control of five germination-related indexes, including germination percentage, was determined using quantitative trait loci (QTL) analysis in Brassica napus , providing insights into drought tolerance (Gad et al, 2021 ). He ( 2021 ) conducted a genetic and transcriptional analysis of seed germination in Chinese cabbage and identified genes related to flavonoid synthesis in the seed coat, embryo development, abscisic acid (ABA) synthesis, regulation of GA, and jasmonic acid (JA) synthesis during the seed germination process.…”

Section: Introductionmentioning

confidence: 99%

A preliminary mapping of QTL qsg5.1 controlling seed germination in melon (Cucumis melo L.)

Yang

Dai

et al. 2022

Front. Plant Sci.

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Melon (Cucumis melo L.) seed germination significantly affects its economic value. Cultivation of melon varieties with high germination ability and seedling vigor is beneficial in large-scale melon propagation. In this study, two melon genotypes differing in their germination ability, P5 with low and P10 with high germination ability, were used to identify the optimal seed germination conditions by evaluating different water immersion times and germination temperatures. The germination rate of the P5 and P10 parental genotypes and their segregating population, consisting of 358 F2:3 families, were evaluated for 2 years to identify their genetic basis. QTL analysis was performed on a high-density genetic map constructed using specific-locus amplified fragment sequencing (SLAF-seq). The germination rate of F1 and F2 populations treated with water immersion for 8 h at 28°C and measured at 48 h showed a normal distribution Genetic mapping carried out using the high-density genetic map revealed eight QTLs in chromosomes 2, 4, 5, 6, and 8 that control melon seed germination, of which 2020/2021-qsg5.1 was consistently significant in both years of experimentation. qsg5.1 explained 15.13% of the phenotypic variance with a LOD of 4.1. To fine map the candidate region of qsg5.1, eight cleaved amplified polymorphism sequence (CAPS) markers were used to construct a genetic map with another 421 F2 individual fruits. The major QTL qsg5.1 was located between SNP53 and SNP54 within a 55.96 Kb interval containing four genes. qRT-PCR gene expression analysis of the candidate genes showed that MELO3C031219.2 (Phosphorus transporter PHO-5) exhibited a significant difference in gene expression between the parental lines at 24, 32, and 48 h after germination, potentially being the underlying gene controlling melon seed germination. These results provide a theoretical basis for the molecular mechanisms controlling melon seed germination and can practically contribute to further improving germination to increase the propagation efficiency of commercial melon cultivars.

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QTL Mapping for Seed Germination Response to Drought Stress in Brassica napus

Cited by 21 publications

References 46 publications

Exogenous SA Affects Rice Seed Germination under Salt Stress by Regulating Na+/K+ Balance and Endogenous GAs and ABA Homeostasis

Exogenous SA Affects Rice Seed Germination under Salt Stress by Regulating Na+/K+ Balance and Endogenous GAs and ABA Homeostasis

Effects of drought and salt stress on seed germination of ephemeral plants in desert of northwest China

A preliminary mapping of QTL qsg5.1 controlling seed germination in melon (Cucumis melo L.)

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