2016
DOI: 10.1242/dev.133173
|View full text |Cite
|
Sign up to set email alerts
|

Quadruple zebrafish mutant reveals different roles of Mesp genes in somite segmentation between mouse and zebrafish

Abstract: The segmental pattern of somites is generated by sequential conversion of the temporal periodicity provided by the molecular clock. Whereas the basic structure of this clock is conserved among different species, diversity also exists, especially in terms of the molecular network. The temporal periodicity is subsequently converted into the spatial pattern of somites, and Mesp2 plays crucial roles in this conversion in the mouse. However, it remains unclear whether Mesp genes play similar roles in other vertebra… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

1
49
2
1

Year Published

2017
2017
2024
2024

Publication Types

Select...
3
3
3

Relationship

1
8

Authors

Journals

citations
Cited by 46 publications
(53 citation statements)
references
References 55 publications
1
49
2
1
Order By: Relevance
“…Next, we were interested in understanding the contribution of SDECs to the developing zebrafish vasculature. To this end we obtained a PM-specific Gal4 transgenic line, Tbx6:Gal4, which has been shown to recapitulate tbx6 mRNA expression (Yabe et al, 2016). We crossed this line to UAS:CRE, which expresses the Cre recombinase upon To complement these results, we performed lineage tracing using a LPM-specific switch line, Draculin-CreERT2 (Henninger et al, 2017), likewise crossing it to Kdrl:CSY for EC-specific reporting.…”
Section: Sdecs Integrate Into the Dorsal Aorta But Do Not Generate Hspcsmentioning
confidence: 99%
See 1 more Smart Citation
“…Next, we were interested in understanding the contribution of SDECs to the developing zebrafish vasculature. To this end we obtained a PM-specific Gal4 transgenic line, Tbx6:Gal4, which has been shown to recapitulate tbx6 mRNA expression (Yabe et al, 2016). We crossed this line to UAS:CRE, which expresses the Cre recombinase upon To complement these results, we performed lineage tracing using a LPM-specific switch line, Draculin-CreERT2 (Henninger et al, 2017), likewise crossing it to Kdrl:CSY for EC-specific reporting.…”
Section: Sdecs Integrate Into the Dorsal Aorta But Do Not Generate Hspcsmentioning
confidence: 99%
“…Wild-type AB* and transgenic TgBAC(kdrl:LOXP-AmCyan-LOXP-ZsYellow) (Zhou et al, 2011) referred to as Kdrl:CSY; Tg(etv2.1:EGFP)zf372 (Veldman and Lin, 2012) referred to as Etv2:GFP; Tg(Tbx6:Gal4FF:GFPnls) (Yabe et al, 2016) referred to as Tbx6:Gal4;…”
Section: Zebrafish Husbandrymentioning
confidence: 99%
“…Primers for cloning other probes of zebrafish genes were as follow: 5’-ATG CTT TCG TGT TTT GCA GAG CAA ACA TAC-3’, 5’-TTA CGA GGA TGA GGT AGA AAG GCT GAG TCC-3’ for paxla ; 5’-ATG CAA ATG GAT CAG ACG TAC GGG GAG GTG-3’, 5’-TTA TGA GTC TGA GAG TCC ATG AAC AGC GCT-3’ for paxlb ; 5’-ATG ATT TCA GCA ATA TCA AGC CCG TGG CTG-3’, 5’-TTA TCT GGG TCC GTA GTC ATA ATT AGT CGG-3’ for tbxl; and 5’-AAA TCT CAC GAT AGG CTC CCT G-3’, 5’-AAA GTA CTC CTG ATT GCA GT-3’ for fgf3 . Immunostaining was conducted as previously reported [64] by using primary antibodies anti-Alcam (1:500; Developmental Studies Hybridoma Bank, University of Iowa City), anti-Kaede (1:400; MBL, PM012), anti-GFP (1:400; Abcam, AB13970), followed by Alexa Fluor-conjugated secondary antibodies (Invitrogen). Bone and cartilage staining of zebrafish larvae was performed as previously described [65].…”
Section: Methodsmentioning
confidence: 99%
“…Generation of rag1 V311fs mutant fish was performed as described previously (Yabe, Hoshijima, Yamamoto, & Takada, 2016). Briefly, the TALEN to target rag1 gene was designed using TAL Effector Nucleotide Targeter 2.0 (https://tale-nt.cac.cornell.edu/node/add/ talen-old), assembled by using a modified Golden Gate Assembly Kit (Sakuma et al, 2013), and cloned into the pCS2 + TAL3DD or pCS2 + TAL3RR (Dahlem et al, 2012).…”
Section: Mutagenesis Using Talenmentioning
confidence: 99%