Qualidade microbiológica de leite UHT caprino: pesquisa de bactérias dos gêneros Staphylococcus, Bacillus e Clostridium

Vittori, Juliano; Schocken-Iturrino, R. P.; Poiatti, Maria Luiza; Pigatto, Caroline Peters; Chioda, Tammy Priscilla; Ribeiro, César Augusto Martins; Garcia, Gisela Rojas; Ragazani, Adriana Valim Ferreira

doi:10.1590/s0103-84782008000300026

Cited by 11 publications

(10 citation statements)

References 3 publications

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“…According to Vittori et al (2008), post-processing contamination may be due to deficiency in packaging sterilization or to recontamination by handlers. The emergence of biofilms in milk processing environments contributes towards an increase in the probability of microbial contamination of processed milk products with deteriorating and pathogenic microorganisms (Marchand et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Escherichia coli O157, Staphylococcus aureus and coliforms in crude and processed bovine milk marketed

Santos¹,

Barros²,

Silva³

et al. 2016

Afr. J. Microbiol. Res.

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Fifty-five (55) samples from five municipalities in the Recôncavo from Bahia region were analyzed between May and July 2015 to evaluate the sanitary quality and the presence of pathogens of crude and processed bovine milk samples. Psichrotrophic, mesophilic and thermophile rates and the count of Escherichia coli, E. coli O157: H7 Staphylococcus aureus and Listeria monocytogenes were done by microbiological methods rapid detection. There was a greater contamination and presence of pathogens in the raw milk when compared to processed milk. However, total coliforms were detected in 14.28%, E. coli in 7.14% and E. coli O157: H7 in 2.04% of processed milk. The enforcement by authorities against the illegal sale of raw milk and the monitoring of steps in milk production up to marketing should be mandatory.

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Section: Resultsmentioning

confidence: 99%

Escherichia coli O157, Staphylococcus aureus and coliforms in crude and processed bovine milk marketed

Santos¹,

Barros²,

Silva³

et al. 2016

Afr. J. Microbiol. Res.

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“…After a serial dilution to 10 -3 , a 20 μL aliquot of each dilution was seeded on nutrient agar with 1.5% NaCl. The plates were incubated at 37 °C for 48 h. 10 Subsequently, the fish scale isolates, as well as the strains of Bacillus spp. from the UFRB (University Federal of the Recôncavo of Bahia) collection that were capable of growth on a crushed scale agar medium [(g.L -1 ): 1 tryptone, 0.5 yeast extract, 1 NaCl, 5 scales, and 15 agar; pH 6.5; 35 °C/48 h)] and milk agar (37 °C/48 h), were selected.…”

Section: Methodsmentioning

confidence: 99%

Formulation of culture media using fish scale bioconversion

Bispo¹,

Mafra²,

Júnior³

et al. 2018

SDRP-JESES

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“…The dilutions were seeded onto Sabouraud agar (Himedia ® ) and malt agar (Himedia ® ), and incubated at 30 ºC for 14 days to evaluate growth and to count the colony forming units (CFU) of filamentous fungi and yeast. The dilutions were subjected to hyperthermia (80 ºC) for 20 minutes followed by water cooling at 5 ºC to activate spores and eliminate vegetative cells, adapting the methods described by Vittori et al (2008). Aliquots were then seeded in 9 cm Petri plates, with nutrient agar (Himedia ® ), and incubated at 30 ºC for up to 72 hours for the CFU counting.…”

Section: Methodsmentioning

confidence: 99%

INHIBITION OF PATHOGENS BY SPOROGENIC BACTERIA ISOLATED FROM HONEY OF Melipona sp. (APIDAE: APINAE: MELIPONINI)

et al. 2016

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-The aim of this study was to isolate sporogenic bacteria from the honey of stingless bees Melipona sp., in dry forest, and to evaluate their antagonistic potential for medicinal employment purposes and animal production. The honey samples were collected in Serra Talhada-PE, where honey was taken from four different hives (in triplicate), totaling 12 samples. The samples were diluted and subjected to 80 ºC for 20 minutes to eliminate vegetative cells. The dilutions were plated onto nutrient agar and incubated at 30 ºC for 72 hours. Then the colony forming units (CFU) were quantified. The samples were also plated onto malt agar and Sabouraud agar, and incubated at 30 ºC for 14 days for the growth of yeast and molds. Total and fecal coliforms were quantified by the most probable number method (MPN). Seven isolates (I) of sporogenic bacteria (Bacillus) were obtained, however only four showed probiotic potential. Isolate I-5 showed the greatest probiotic potential and inhibited the growth of Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa, Salmonella sp., and Staphylococcus aureus. The growth of the Sarcina sp. was not inhibited by any isolate. No yeast, molds or coliforms were found. The Melipona sp. honey is a source of spore-forming bacteria and is antagonistic to microorganisms that contaminate honey. It has good microbiological quality.Keywords: Stingless bee. Bacillus. Caatinga. INIBIÇÃO DE PATÓGENOS POR BACTEIAS ESPORULANTES ISOLADAS DE MEL DE Melipona sp. (APIDAE: APINAE: MELIPONINI)RESUMO -Este trabalho teve como objetivo isolar bactérias esporulantes a partir do mel de Melipona sp. em ambiente de caatinga e avaliar o seu potencial antagonista com vistas à aplicação na medicina e produção animal. As amostras de méis foram coletadas no município de Serra Talhada-PE, onde o mel foi retirado de quatro colméias diferentes (em triplicata), totalizando 12 amostras que amostras foram diluídas (10 -1 a 10 -5 ) e submetidas a 80 ºC por 20 minutos e resfriadas a 5 ºC para indução de esporos e eliminação de células vegetativas. Em seguida, as diluições foram semeadas em Agar Nutriente e incubadas a 30 º por 72 horas para contagem das unidades formadoras de colônias (UFC). Antes do tratamento térmico foram realizados testes para verificar a presença de fungos filamentosos, leveduras e coliformes totais e termotolerantes. As diluições foram semeadas em Agar Malte e Agar Sabouraud e incubadas a 30 ºC por 14 dias. Coliformes totais e termotolerantes foram quantificados pelo método do número mais provável (NMP). Foram obtidos sete isolados (I) de bactérias esporulantes (Bacillus), porém apenas quatro mostraram algum potencial antagonista. O isolado I-5 apresentou o maior potencial antagonista, inibindo o crescimento de Escherichia coli, Klebsiella sp., Pseudomonas aeruginosa, Salmonella sp. e Staphylococcus aureus. Apenas Sarcina sp. não foi inibida. Não foram encontrados fungos filamentosos, leveduras ou coliformes nas amostras. O mel de Melipona sp. é uma fonte de bactérias esporulantes resistentes a el...

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Qualidade microbiológica de leite UHT caprino: pesquisa de bactérias dos gêneros Staphylococcus, Bacillus e Clostridium

Abstract: RESUMO Com o objetivo de avaliar a qualidade microbiológica do leite UHT caprino, 100 amostras provenientes das regiões Sul e Sudeste do país foram analisadas através do isolamento e da identificação de bactérias do gênero

Cited by 11 publications

References 3 publications

Escherichia coli O157, Staphylococcus aureus and coliforms in crude and processed bovine milk marketed

Escherichia coli O157, Staphylococcus aureus and coliforms in crude and processed bovine milk marketed

Formulation of culture media using fish scale bioconversion

INHIBITION OF PATHOGENS BY SPOROGENIC BACTERIA ISOLATED FROM HONEY OF Melipona sp. (APIDAE: APINAE: MELIPONINI)

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