Qualitative analysis of the viability of autogenous fat grafts grafted in different environments of interstitial pressure. Preliminary results and description of a new experimental model in mini-pigs

Abstract

Purpose: To evaluate the feasibility of an experimental model of autologous fat graft (AFG) in different interstitial pressure (IP) environments. Methods: Three mini-pigs(Minipig-BR) with age of 8 months (weight: 25-30 kg) were used. AFG were collected from the bucal fat pad, and grafted in the intramuscular pocket (biceps femoralis muscle). IP model was based on a fusiform ressection followed by primary closure "under tension". A blood pressure catheter located in the intramuscular region connected to a pressure module was applied to quantify IP. Results: The mean operative time was 236 min (210 -272 min). All the AFG and muscular segments were removed successfully. Average interstitial pressure CP and H were 3 and 10.6 mmHg respectively. The AFG were biopsied for histopathological analysis 30 days after graft. Hematoxylin-eosin staining and immunohistochemical analyzes (TNF-alpha, CD31 and Perilipine with monoclonal antibodies) were employed. Conclusion: The data show that minipigs model could be used as a recipient site for autologous fat graft techniques and allow the development of studies to explore the AFG intake and pathophysiology response.

Contouring and augmentation of the temple using stromal vascular fraction gel grafting

“…At the 12 months follow-up in this trial, the retention rates in the SVF-gel and Coleman's fat groups were 41.2% ± 8.4% and 32.6% ± 8.8%, respectively, which were lower than those of other studies. This difference could be due to the injection layer ( 20 ). Our findings for volume retention of temple augmentation with Coleman's fat at 12 months follow-up coincide with prior observations.…”

Utilizing a Porcine Fat Grafting Model for Translational Research: Surgical Approach, Complications, and Expected Outcomes

“…Moreover, the availability of established imaging modalities allows for the comprehensive evaluation of graft survival, vascularization, tissue integration, and potential complications. Consequently, swine have been utilized in prior fat transfer studies [3][4][5][6]. A close review of this literature, however, demonstrates a significant variability in the models, outcomes, and techniques [3][4][5][6].…”

“…Consequently, swine have been utilized in prior fat transfer studies [3][4][5][6]. A close review of this literature, however, demonstrates a significant variability in the models, outcomes, and techniques [3][4][5][6]. There is a wide range of pig breeds available with significant anatomic, physiologic, and histologic variabilities between them.…”

“…Furthermore, the geographic location plays a role, with some breeds available only in certain regions or in a very limited supply. From a technical perspective, we identified variabilities in the surgical approach, harvest, processing, and recipient sites [3][4][5][6], and although the extant literature does explore the anatomic and tissue qualities of porcine fat, it is often with a focus on the agricultural productivity and nutritional science rather than from a translational perspective [3][4][5][6].…”