Qualitative and Quantitative Analysis (GC-MS) of Methanol Extract of Crataeva nurvala stem bark

Aathira, KK; Bibu, John Kariyil; Dhanusha, G; Haima, JS; Sujith, S.; Shynu, M.; Nisha, A. R.

doi:10.51966/jvas.2021.52.2.135-141

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…All the data were obtained by collecting the full-scan mass spectra within the scan range 50-500 atomic mass units (amu). Compounds were identified using the National Institute of Standards and Technology MS Search 2.0 library (Aathira et al, 2021).…”

Section: Gas Chromatography-tandem Mass Spectrometry (Gc-ms/ms) Analysismentioning

confidence: 99%

Qualitative and quantitative phytochemical analysis of methanolic extract of Magnolia champaca leaves

Shankar¹,

John²,

Nisha³

et al. 2023

Journal of Veterinary and Animal Sciences

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Plants remain a vibrant source of diverse bioactive phytochemicals that are secondary metabolites protecting them from infections and predations. Magnolia champaca is reported to possess a multitude of phytochemicals. In the present study, the phytochemical constituents of the methanolic extract of Magnolia champaca leaves were analysed qualitatively and with gas chromatography-tandem mass spectrometry (GC-MS/MS). Fourier transform infrared (FTIR) spectroscopic analysis was performed to identify the chemical nature of the extract and to find structurally similar compounds. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, terpenes, glycosides, phenols, steroids, tannins and saponins. FTIR spectroscopic analysis revealed structurally related compounds. GC-MS/MS analysis revealed the presence of 99 diverse compounds with varied biological activities, among which 1,2,4-butanetriol, n-hexadecanoic acid, cis vaccenic acid, phytol, trans longipinocarveol and caryophyllene oxide were found predominantly. Thus, the identification and characterisation of the phytochemicals in the extract favour the development of novel therapeutic agents.

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Section: Gas Chromatography-tandem Mass Spectrometry (Gc-ms/ms) Analysismentioning

confidence: 99%

Qualitative and quantitative phytochemical analysis of methanolic extract of Magnolia champaca leaves

Shankar¹,

John²,

Nisha³

et al. 2023

Journal of Veterinary and Animal Sciences

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“…Subsequently, it was allowed for drying in the rotary evaporator. The yield of extract was calculated from the extract obtained (Aathira et al, 2021). Finally, the resultant product was stored in refrigerator for further study.…”

Section: Aqueous Extract From Seeds Of S Indicummentioning

confidence: 99%

Phytochemical analysis and biosynthesis of silver nanoparticles from aqueous extract of seeds of Sesamum indicum

Keerthika¹,

Nisha²,

Nair³

et al. 2022

Journal of Veterinary and Animal Sciences

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Plants have been in use in medicine from time immemorial. Sesamum indicum, an ancient seed crop, has been widely used mainly for its oil. It is regarded as highly beneficial for its nutritive value and therapeutic effect. It is known to have anti-allergic, anti-inflammatory, anticancer, analgesic and many other pharmacological activities. In this study, seeds of S. indicum were subjected to aqueous extraction followed by evaluation for the presence of phytochemical constituents. The aqueous extract of seeds of S. indicum after phytochemical testing, was found to contain alkaloids, phenols, steroids, flavonoids, diterpenes, glycosides and tannins. Following this, silver nanoparticles were biosynthesised from the extract, where the phytochemicals acted as reducing and capping agents for the nanoparticles formed. The synthesised nanoparticles were characterised by using UV-visible spectroscopy, energy dispersive X-ray spectroscopy, field emission scanning electron microscopy and X-ray diffraction. The synthesis of nanoparticles from plant extract is gaining importance now-a-days owing to the effectual activity elicited by them when compared to activity of plant compounds alone.

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“…Separation of the crude methanol extract was also achieved using gas chromatography and qualitative analyses of the constituents was determined via phytochemical screening, Fourier Transform Infrared spectroscopy (FT-IR) and mass spectrometry. The separation capabilities of gas chromatography were coupled with the capacity for qualitative and quantitative detection of mass spectrometry to provide accurate substance identification [18]. KBr pellet method was used as a carrier for the sample in the infrared spectrum because it is optically transparent with a transmittance of 100% in the range of IR measurement (4000-400 cm -1 ) so that no interference in the absorbance would occur [19].…”

Section: Introductionmentioning

confidence: 99%

GC-MS profile and antimicrobial activities of extracts from root of Senna occidentalis Linn.

Amako,

Mgbemena,

Odo

2023

Ovidius University Annals of Chemistry

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We considered the extraction of plant materials from Senna occidentalis root, and its fractionation monitored by bioassay towards isolating its bioactive principles. Pulverized root sample of Senna occidentalis (fam. leguminaceae) was extracted with methanol using a maceration method. The crude methanol extract (MSo) 6.06 g was partitioned into petroleum ether and ethyl acetate to yield their respective fractions viz: petroleum ether fraction (1.20 g), ethyl acetate fraction (1.86 g) and methanol fraction (2.92 g). The crude methanol extract was analyzed using phytochemical screening, infrared spectroscopy, and gas chromatography/mass spectrometry while the fractions were examined for antimicrobial properties. Phytochemical screening indicated the presence of tannins, phenolics, alkaloids, flavonoids, saponins, cardiac glycosides, phlobatannin, and absence of steroids. The infrared spectrum revealed a broad absorption band at 3437.26 cm-1 due to O-H symmetric stretch in polymeric alcohols, and absorption frequency of 1640.51cm-1 of moderate intensity due to C=O stretch. Enols are easily identified by the broad H-bonded O-H stretch absorption and low C=O stretch frequency as in β-keto enolic esters and phenol acetates, -CO-O-C=C-, (1690-1650 cm-1). Gas chromatography/mass spectrometry identified the presence of ten compounds including n-hexadecanoic acid (23.76%), linoleic acid (1.64%), E-9-tetradecenoic acid (4.88%), octadecanoic acid, 2-(2 hydroxyethoxy) ethyl ester (6.24%) and E-2-octadecadecen-1-ol (13.74%). Similar broad spectrum antimicrobial activities were manifested by methanol and ethyl acetate fractions. Escherichia coli and Bacillus cereus were the most susceptible with the highest zone of inhibition of 30 mm and 28 mm respectively at minimum inhibition concentration of 1.35 x103 μg/ml. The methanol fraction has the highest potency against the tested pathogens whereas the petroleum ether fraction exhibited activity only on gram negative pathogens. The antimicrobial activities observed in these fractions suggest the presence of active chemical components in the crude methanol root extract of Senna occidentalis thus provides a potential source of novel antimicrobial agents. Further work is however, required to isolate and characterize these bioactive principles.

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Qualitative and Quantitative Analysis (GC-MS) of Methanol Extract of Crataeva nurvala stem bark

Cited by 5 publications

References 12 publications

Qualitative and quantitative phytochemical analysis of methanolic extract of Magnolia champaca leaves

Qualitative and quantitative phytochemical analysis of methanolic extract of Magnolia champaca leaves

Phytochemical analysis and biosynthesis of silver nanoparticles from aqueous extract of seeds of Sesamum indicum

GC-MS profile and antimicrobial activities of extracts from root of Senna occidentalis Linn.

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