Qualitative and quantitative analysis of peanut oil triacylglycerolsby reversed-phase liquid chromatography

Semporé, G.; Bézard, J.

doi:10.1016/s0021-9673(01)93474-0

Cited by 42 publications

(10 citation statements)

References 19 publications

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“…In principle, mobile phases are composed of a "weak" organic solvent, mostly acetonitrile and a "strong" organic modifier, mostly acetone or alternatively chlorinated solvents (dichloromethane, chloroform, 1,2-dichloroethane), benzene, or tetrahydrofurane, which is added to improve the solubility of TGs in the mobile phase. The most widely employed solvent system is a mixture of acetonitrile with acetone [93,112,117,126,136,140]. Acetone improves the separation of critical pairs of TGs and acetonitrile is able to interact with the p electrons of double bonds, thereby effecting separation of unsaturated species.…”

Section: Podlaha and Töregårdmentioning

confidence: 99%

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Triacylglycerol profiling by using chromatographic techniques

Buchgraber

Ulberth

Emons

et al. 2004

Euro J Lipid Sci & Tech

189

129

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Triacylglycerol profiling by using chromatographic techniquesTriacylglycerols (TGs) make up the major part of naturally occurring fats and oils. The composition and fine structure of TGs determine to a large extent the functionality of fats and oils as food ingredients and the physiological effects of fats and oils as component of the human diet. Analysis of intact TGs is usually performed by chromatographic methods. In this article the application of gas-liquid chromatography, highperformance chromatography in normal and reversed phase mode, thin-layer chromatography and supercritical fluid chromatography for the qualitative and quantitative determination of TGs is reviewed. Emphasis is put on those factors that are decisive for obtaining reliable quantitative data. Furthermore, techniques for the stereospecific analysis of the fatty acid distribution along the glycerol backbone of TGs are presented briefly.

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Section: Podlaha and Töregårdmentioning

confidence: 99%

“…Many published methods employ RI detectors and isocratic solvent systems [88,90,94,97,111,117,126,129,132,135,140]. RP-HPLC using acetone/acetonitrile as mobile phase and RI detection was found to be an effec-…”

Section: Samplementioning

confidence: 99%

Triacylglycerol profiling by using chromatographic techniques

Buchgraber

Ulberth

Emons

et al. 2004

Euro J Lipid Sci & Tech

189

129

View full text Add to dashboard Cite

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“…For a given chromatographic system, according to the principle of additivity, the retention (relative to trioleate retention, α, or selectivity) of TG composed by acids A, B and C may be described by Equation [15]: logα(ABC) = logα(A) + logα(B) + logα(C)…”

Section: Some Remarks About Increment Approachmentioning

confidence: 99%

“…However, there exist strict regularities in TGs retention. Equivalent carbon numbers (ECN) connect solute retentions with that of synthetic TGs composed by saturated fatty acids substituents [14,15]. ECN depend upon structure of all moieties, are additive and so their retention is predetermined.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Separation of Seed Oil Triglycerides Containing Isomeric Conjugated Octadecatrienoic Acid Moieties by Reversed-Phase HPLC

et al. 2017

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Abstract:Relative retention analysis and increment approach were applied for the comparison of triglycerides (TGs) retention of a broad set of plant seed oils with isomeric conjugated octadecatrienoic acids (CLnA) by reversed-phase HPLC for "propanol-2-acetonitrile" mobile phases and Kromasil 100-5C18 stationary phase with diode array detection (DAD) and mass spectrometric (MS) detection. The subjects of investigation were TGs of seed oils: Calendula officinalis, Catalpa ovata, Jacaranda mimosifolia, Centranthus ruber, Momordica charantia, Trichosanthes anguina, Punica granatum, Thladiantha dubia, Valeriana officinalis, and Vernicia montana. It was found that a sequence of elution of TGs of the same types is the same without any inversions for full range of mobile phase compositions: punicic (C18:3 9Z11E13Z ) < jacaric (C18:3 8Z10E12Z ) < catalpic (C18:3 9E11E13Z ) < α-eleostearic (C18:3 9Z11E13E ) < calendic (C18:3 8E10E12Z ) < β-eleostearic (C18:3 9E11E13E ) < all-E calendic (C18:3 8E10E12E ) acids. TGs and fatty acid compositions were calculated for all oil samples. Regularities of solute retentions as a function of isomeric conjugated octadecatrienoic acid moiety structure are discussed. Thus, it was proven that it is possible to differentiate TGs of complex composition with moieties of all natural CLnA by retention control accomplished by electronic spectra comparison, even though there are only three types of electronic-vibration spectra for seven isomeric CLnA.

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“…Peanut seed may range in oil content from 44% to 56% with an average of 50% [8]. The oil contains 44.5% oleic acid, 32.3% linoleic acid, and 13.9% palmitic acid [9] and hence it has an excellent keeping quality. Therefore, it is expected that ACC and LOs would play important roles in determining the quantity and quality of fat storage reserves in the crop.…”

Section: Introductionmentioning

confidence: 99%

Nucleotide-Dependent Reprogramming of mRNAs Encoding Acetyl Coenzyme A Carboxylase and Lipoxygenase in Relation to the Fat Contents of Peanut

2009

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The nucleotide-dependent reprogramming of the mRNAs encoding acetyl coenzyme A carboxylase (ACC, EC 6.4.1.2) and lipoxygenase (LO, EC 1.13.11.12) was studied by Northern analysis. Equal concentrations of total RNA from the peanut (Arachis hypogaea L. cv. "Valencia") seedlings were probed with those glutamate dehydrogenase (GDH)-synthesized RNAs that were homologous to the mRNAs encoding ACC and LO. Treatment (4NTPs solution) of the peanuts increased the abundance of the mRNAs encoding the LO and ACC about 5 and 10 folds respectively but decreased the fat accumulation by 37% compared with the control peanut. Treatments (pyrimidine NTPs)of the peanut induced up to 60% more fat accumulation than the control. The high fat (40.0-44.0%) contents of the UTP-, and 3NTP-treated peanuts were characterized by very high abundance of the mRNA for ACC, but low abundance of the mRNA for LO. The inverse relationship between the abundance of the mRNAs encoding ACC and LO compared with the seedlings fat contents was due to the plus/minus strands sequence homologies between the Northern probes for ACC and LO. Therefore, LO, ACC, and the GDH-synthesized RNAs participated in the regulation of the fat contents. These results could be useful in the environmental manipulation of the quantity and quality of peanut oil.

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Qualitative and quantitative analysis of peanut oil triacylglycerolsby reversed-phase liquid chromatography

Cited by 42 publications

References 19 publications

Triacylglycerol profiling by using chromatographic techniques

Triacylglycerol profiling by using chromatographic techniques

Comparison of Separation of Seed Oil Triglycerides Containing Isomeric Conjugated Octadecatrienoic Acid Moieties by Reversed-Phase HPLC

Nucleotide-Dependent Reprogramming of mRNAs Encoding Acetyl Coenzyme A Carboxylase and Lipoxygenase in Relation to the Fat Contents of Peanut

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