Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts

Boutaoui, Nassima; Zaiter, Lahcene; Benayache, Fadila; Carradori, Simone; Cesa, Stefania; Giusti, Anna Maria; Campestre, Cristina; Menghini, Luigi; Innosa, Denise; Locatelli, Marcello

doi:10.3390/molecules23020463

Cited by 32 publications

(54 citation statements)

References 27 publications

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“…Phenolic acids, mainly rosmarinic acid and its derivatives dominated T. algeriensis extract while the phenolic diterpene, carnosol, and the methylated flavonoid, salvigenin, was the major secondary metabolite in the T. fontanesii extract. [11], e [12].…”

Section: Resultsmentioning

confidence: 99%

Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison

et al. 2020

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This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae). Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract. Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway. In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts. Results revealed that both studied Thymus species exhibited antioxidant, anti-inflammatory, analgesic, and antipyretic effects. They showed to be a more potent antioxidant than ascorbic acid and more selective against cyclooxygenase (COX-2) than diclofenac and indomethacin. Relatively, the T. fontanesii extract was more potent as COX-2 inhibitor than T. algeriensis. In conclusion, Thymus algeriensis and Thymus fontanesii may be interesting candidates for the treatment of inflammation and oxidative stress-related disorders.

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Section: Resultsmentioning

confidence: 99%

Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison

et al. 2020

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“…The total carotenoids and chlorophylls a and b determination for the Atriplex sample was carried out according to Solovchenko and co-workers (2001) with slight modifications [ 18 , 19 ]. The sample was powdered (50 mesh) with mortar and pestle in 5 mL of chloroform-methanol (2:1, v / v ) mixture containing 0.01% BHT to limit peroxidation.…”

Section: Pigment Determinationmentioning

confidence: 99%

Atriplex mollis Desf. Aerial Parts: Extraction Procedures, Secondary Metabolites and Color Analysis

et al. 2018

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A method using high-performance liquid chromatography coupled with a photodiode array detector was proposed for the rapid characterization of different phenolic constituents from the extracts of Atriplex mollis aerial parts. Atriplex species are known for their multiple biological activities, but no information is available in the literature about A. mollis. With the aim to firstly characterize the main secondary metabolites of this plant, so as to orient better the biological evaluation, we applied three different extraction procedures and compared the chromatographic results. Microwave-assisted extraction gave the best yield and recovery of important compounds such as gallic acid, catechin, chlorogenic acid, p-OH benzoic acid, rutin, sinapinic acid, t-ferulic acid, naringenin and benzoic acid. These constituents belong to three important chemical classes: phenolic acids, flavonoids and monoterpenes. Color evaluation and analysis of chlorophylls (a and b) and carotenoids complete the preliminary profile of this plant. From these analyses, Atriplex mollis is a source of bioactive compounds (especially rutin, t-ferulic acid and gallic acid) and could be recommended as a plant of phyto-pharmaceutical relevance, opening new perspectives on this salt-tolerant plant.

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“…The essential oils of several species of thyme have already proved their antibacterial and antifungal properties [4][5][6][7]. In continuation of our studies on Thymus species [8,9], we report herein, our results concerning the chemical composition of the essential oil of the aerial parts of Thymus ciliatus (Desf.) Benth.…”

Section: Introductionmentioning

confidence: 79%

Chemical Composition of the Essential Oil of Aerial Parts of Thymus ciliatus (Desf.)

Achoub

Zaiter

Benayache

et al. 2019

Acta Scientifica Naturalis

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The essential oil of the aerial parts of Thymus ciliatus (Desf.) belonging to the Lamiaceae family, was obtained by steam distillation and analyzed by GC-FID and GC-MS. 75 components were identified corresponding to 95.57% of the total oil. The major constituents of the oil were: elemol (6.80%), carvacrol (5.86%), γ-muurolene (5.18%), β-sesquiphellandrene (5.09%), bicyclogermacrene (5.04%), β-pinene (4.49%) and curcumene (4.20%), together with other compounds at relatively low levels: 1,8-cineol (3.66%), β-eudesmol (2.92%), β-bisabolene (2.81%), β-silinene (2.75%), camphor (2.64%), germacrone (2.34%), α-zingiberene (2.12%), δ-cadinene (2.08%), caryophyllene oxide (1.90%), spathulenol (1.88%), □-caryophyllene (1.88%), ar-turmerone (1.79%), α-pinene (1.52%), limonene (1.52%), selina-4,11-diene (1.46%), curzerenone (1.41%), germacrone B (1.37%), bornyl acetate (1.31%), β-farnesene (1.28%), borneol (1.23%), myrtenal (1.16%), zingiberenol (1.15%) and sabinene (1.13%). These results differ from those of previous studies reported on this species collected from other regions of Algeria and Morocco.

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Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts

Cited by 32 publications

References 27 publications

Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison

Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison

Atriplex mollis Desf. Aerial Parts: Extraction Procedures, Secondary Metabolites and Color Analysis

Chemical Composition of the Essential Oil of Aerial Parts of Thymus ciliatus (Desf.)

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