Qualitative tests for preliminary phytochemical screening: An overview

Shaikh, Junaid R; Patil, MK

doi:10.22271/chemi.2020.v8.i2i.8834

Cited by 389 publications

(305 citation statements)

References 17 publications

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“…Afterward, 1 ml of 95% ethanol and few Potassium hydroxide (KOH) pellets were added. The presence of a pink color formation confirms the existence of amino acids [12].…”

Section: Biuret Testmentioning

confidence: 55%

Comparative Studies of Phytochemical and Antioxidant Activity of in Vivo Plant and in Vitro Callus Extract of Cardiospermum Halicacabum L.

Thaniarasu

Logeshwari

2021

Asian J Pharm Clin Res

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Objective: The present investigation focuses on the use of Cardiospermum halicacabum L. in their phytochemical and biological activities. Methods: In this study, in vivo stem and in vitro callus ethanolic extracts of C. halicacabum were tested for their phytochemical attributes by qualitative method, Fourier transform infrared (FTIR), antioxidant, antibacterial, and bioactive compound properties. The bactericidal activity of the in vivo stem and in vitro callus extract has been evaluated in both Gram+ve and Gram-ve microorganisms using the disk diffusion method. Results: The highest frequency (78%) of well developed, dark green organogenic callus was induced from stem explant on Murashige and Skoog (MS) medium supplemented with 0.7 mg/l 2,4-Dichlorophenoxyacetic acid (2, 4-D) and 0.5 mg/l benzyl adenine (BA). The results of FTIR spectra confirmed the presence of functional groups in wild stem and in vitro callus extract of C. halicacabum with various peaks. The total phenolic content in ethanolic extract of in vivo plant and in vitro callus was 80.46 mg gallic acid equivalent (GAE)/g dry weight and 76.4 mg GAE/g dry weight, respectively. The highest percentage of tannins was measured at 78.03 in wild stem ethanol extracts followed by 75.22 in callus extract. The antioxidant activity of 2,2-diphenyl-2- picrylhydrazyl (DPPH) ethanol extract was found to be 206.54 μg/ml. IC50 values of the stem extracts of C. halicacabum are 306 μg/ml and 286 μg/ml in callus extract, respectively. Antibacterial activity of the ethanol extract was higher for Staphylococcus aureus (S. aureus) with a 17 mm zone of inhibition. Conclusion: The present investigation recommended that the callus ethanolic extract function as a good source of biologically active compounds and natural antioxidants.

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“…Afterward, 1 ml of 95% ethanol and few Potassium hydroxide (KOH) pellets were added. The presence of a pink color formation confirms the existence of amino acids [12].…”

Section: Biuret Testmentioning

confidence: 55%

Comparative Studies of Phytochemical and Antioxidant Activity of in Vivo Plant and in Vitro Callus Extract of Cardiospermum Halicacabum L.

Thaniarasu

Logeshwari

2021

Asian J Pharm Clin Res

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“…[5] Hager's Test Few drops of Hager's reagent were added to the sample solution and observed for yellowish precipitate at the bottom of the test tube. [6]…”

Section: Mayer's Testmentioning

confidence: 99%

“…Wagner's Test Few drops of Wagner's reagent were added to the sample solution and observed for reddish brown precipitate at the bottom of the test tube [5]. Hager's TestFew drops of Hager's reagent were added to the sample solution and observed for yellowish precipitate at the bottom of the test tube [6].…”

mentioning

confidence: 99%

Basic Organoleptic and Phytochemical Study on Essential Ingredients of Mahadalu Anupana (A Sri Lankan Traditional Recipe)

Weerasekera

2021

Int J Ayu Pharm Res

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Mahadalu anupana is one of the most common Dalu anupana (fluid vehicles for medicine which is prepared by tender leaves and some other additives) of the traditional medical system in Sri Lanka and this Anupana is one of Anupana mentioned under Chandra kalka. Mahadalu anupana is used in many ailments with or without Kalka in traditional medical system. This research work is mainly based on the main twelve ingredients of the formulation of Mahadalu anupana without the additives (sugar, ghee, orange juice, breast milk, sandalwood and Strychnos potatarum) which has mentioned in the authentic text of Vatika prakaranaya/ Deshiya beheth guli kalka potha in Sri Lankan traditional medicine. In this study, organoleptic properties and selected phytochemical studies were performed for future standardization of drug. Phytochemicals are bioactive compounds obtained from the plants and has therapeutic potential. The organoleptic properties indicated of this Anupana as follow; colour: brownish green, odour: strong aromatic, taste: astringent and the texture were liquid. The phytochemical analysis study was done for the water extract of Mahadalu anupana. This study has revealed that the presence of alkaloids, tannins, flavonoids, saponins, terpenoids, glycosides and carbohydrates qualitatively in main ingredients of Mahadalu aupana but steroids and proteins were not reported. It was concluded that the Mahadalu anupana (without additives) were rich in phytochemicals with medicinal significant and this study results can be utilized in future consistency studies of Mahadalu anupana.

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“…These phytoconstituents work with nutrients and fibers to form an essential part of the protection mechanism against specific diseases and stress conditions. Qualitative analysis as well as quantitative phytochemical analysis are the important application of biomedicine in pharmaceutical industries [41,42]. The phytochemical analysis was very useful in identifying chemical compounds in plant material which led to their quantitative estimate and the position of the pharmacy.…”

Section: Phytochemical Evaluationmentioning

confidence: 99%

Boswellia Serrata Roxb. – A Bioactive Herbs With Various Pharmacological Activities

Mishra

Bishnoi

Maurya

et al. 2020

Asian J Pharm Clin Res

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Herbal medicine has become a medicinal as well as the economic aspect of global significance. While the use of these herbal medicines has increased, there are several questions about their consistency, protection, and effectiveness in different countries. Boswellic acid (BA) is one of the active constituents obtained from plant Boswellia serrata (BS) family Burseraceae. The oleoresin gum of the plant is also known as Salai guggul, Indian olibanum, or Indian frankincense. Boswellia species comprises a variety of phytochemical components, essential oil, BA such as keto-BA, beta-BA, or acetyl keto-BA. This variety of constituents isolated from the plant using various extraction processes such as hydrodistillation, percolation, and ultraviolet-assisted extraction or solvent extraction. The active constituent has different biological activities such as antidiuretic, anticancer, anti-inflammatory, or antitumor activity. This review seeks to update information on plant BS with its medicinal uses, isolation process in the traditional or Indian system of medicine, and justify its use on modern scientific parameters.

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Qualitative tests for preliminary phytochemical screening: An overview

Cited by 389 publications

References 17 publications

Comparative Studies of Phytochemical and Antioxidant Activity of in Vivo Plant and in Vitro Callus Extract of Cardiospermum Halicacabum L.

Comparative Studies of Phytochemical and Antioxidant Activity of in Vivo Plant and in Vitro Callus Extract of Cardiospermum Halicacabum L.

Basic Organoleptic and Phytochemical Study on Essential Ingredients of Mahadalu Anupana (A Sri Lankan Traditional Recipe)

Boswellia Serrata Roxb. – A Bioactive Herbs With Various Pharmacological Activities

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