Quality evaluation of meshed split-thickness skin grafts stored at 4°C in isotonic solutions and nutrient media by cell cultures

Li, Zhe; Overend, Christine; Maitz, Peter

doi:10.1016/j.burns.2012.02.002

Cited by 13 publications

(12 citation statements)

References 26 publications

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“…Over a longer period, close to 620 days, there would be no viability (0%) of cryopreserved epithelium. The present data confirm previous studies which have shown that the storage of the tissue 4ºC 31,35 and -70ºC 30 result in a reduction of viability after a few weeks. In the clinical situation of need storage for longer periods, the best indication would be the use of liquid nitrogen in order to maintain proper temperature and thus increase tissue viability.…”

Section: Discussionsupporting

confidence: 92%

An outcome analysis and long-term viability of cryopreserved cultured epidermal allografts: assessment of the conservation of transplantable human skin allografts

et al. 2013

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PURPOSE:To assess the viability of cultured epithelium and preserved by freezing for periods varying from one month to one year. METHODS:Samples of cultured epithelium were incubated in cryoprotectant medium (Group A), packed in aluminum envelopes and packed in polystyrene boxes. The boxes were subjected to a temperature of-70 o C. After freezing for a period of time ranging from one to 12 months, cultured epithelial samples were assessed for their viability by vital staining (Trypan blue) and metabolic analysis based on glucose consumption and lactate production. Samples of not frozen cultured epithelium (Group B) were also tested for viability and the results obtained were used as comparison parameter for the variation of viability. RESULTS:Statistical analysis between the group A and B indicate that the mean age of the donors (p=0.51) and the culture time (p=1.18) showed no statistical difference. In 30 days we obtained 37% of the original viability of cultured epithelium, 25% at six months and one year, less than 15%. This trend was confirmed statistically with a reduction of approximately 1.8% of the original viability epithelium cultured every 30 days of storage. In the analysis by lactate production, similar results were observed. In the analysis by the glucose consumption results were not significant. The viability indices show statistically significant difference between the group A and B (p<0.0001). CONCLUSIONS:Although cryopreserved cultured epithelium showed significant reduction of viability, all samples remained viable. It was also found that the viability of cryopreserved cultured epithelial decreased as a function of storage time.

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Section: Discussionsupporting

confidence: 92%

An outcome analysis and long-term viability of cryopreserved cultured epidermal allografts: assessment of the conservation of transplantable human skin allografts

et al. 2013

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“…Indeed, storage of tissues at +4°C had shown to reduce viability in a few weeks. 15,18,47,49 Similar results were observed with storage at −70°C. 46,50 Most studies have investigated the viability of skin allografts cryopreserved for 2 and 4 weeks: very few have focused on long-term storage (≥2 months).…”

Section: Storage Temperature and Allograft Viabilitysupporting

confidence: 79%

“…[43][44][45][46][47][48][49] In general, the decay of cell viability is inversely correlated with storage time and depends on storage temperature. Most authors agree that acceptable viability is obtained with a storage temperature of −80°C or below (−130°C).…”

Section: Storage Temperature and Allograft Viabilitymentioning

confidence: 99%

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Current insights into skin banking: storage, preservation and clinical importance of skin allografts

Tognetti¹,

Pianigiani²,

Ierardi³

et al. 2017

BSAM

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Donor skin and dermal grafts are used in several types of loss of substance for different clinical purposes. As biological physiological medication, donor skin grafts can promote re-epithelization, shorten healing time, alleviate pain and protect dermal and subcutaneous structures such as cartilage, tendons, bones and nerves. Though a great variety of dermal matrices and skin equivalents, both synthetic and semisynthetic, are now available for wound treatment, viable human skin allografts remain an important therapeutic choice for extensive deep burns and hard-to-heal wounds. In such cases, viable skin allografts have significantly better clinical outcomes than unviable human-derived allografts or synthetic medications. The demand for human-derived skin bioproducts continues to be a reason for the existence of skin banks. Skin bank organization is complex and requires continuous updating. Careful donor selection, thorough microbiological and serological donor screening for transmissible diseases and rigorous quality control during tissue preparation are necessary to minimize the risk of transmission of pathogenic agents. Skin banks must also observe standardized reproducible procedures to ensure tissue traceability and biological safety in all phases of processing and to avoid new biological contamination. Constant training and periodic checks are needed to keep skin bank operators attentive and responsible. Finally, skin banks should guarantee collection and storage of highly viable skin. Here, we discuss available tissue storage methods and the different types of skin bioproducts.

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“…cluded that the cells may have adapted protective mechanisms under low-temperature conditions, allowing them to maintain function and morphology. They stated that the stored meshed split-thickness skin grafts should be used within 7 days as the viable number of cells, cell growth efficiency, and keratinocyte colony forming efficiency decreased rapidly after this period(Li, Overend, Maitz, & Kennedy, 2012). They stated that cell viabil-ity in grafts decreased by 50% from Day 3 to Day 14.…”

mentioning

confidence: 99%

Skin graft storage in platelet rich plasma (PRP)

Gökkaya

Görğü

Astarci

et al. 2019

Dermatologic Therapy

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Storage of skin grafts for later use is one of the standard applications in surgery. It is the most preferred method to maintain at +4 C in refrigeration after wrapping the surplus grafts into sterile gauze pad moistened with saline. Although there are many studies on the storage of skin grafts, less is known about storing skin grafts with PRP.Twenty-five pieces of 1 × 1 cm 2 partial thickness skin graft were harvested from 12 patients during the reduction mammoplasty operation. Twenty-four grafts were divided into 4 groups, and each group consisted of 6 grafts, 1 graft was analyzed as Day 0. Grafts in Group 1, 2, and 3 were wrapped by sterile gauze pad moistened by either saline (Group 1) or Hartman (Group 2) or PRP (Group 3). Grafts were analyzed macroscopically and microscopically. There were no significant differences between media for the first 10 days. Decrease in viability was less in saline and PRP wrapped grafts at 20 day, viability decreased significantly in all environments after 20 days.Although there was no significant difference in saline or PRP storage, it was observed macroscopically that the grafts stored in the PRP appeared better. K E Y W O R D Sgraft preservation, platelet rich plasma, skin graft storage

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Quality evaluation of meshed split-thickness skin grafts stored at 4°C in isotonic solutions and nutrient media by cell cultures

Cited by 13 publications

References 26 publications

An outcome analysis and long-term viability of cryopreserved cultured epidermal allografts: assessment of the conservation of transplantable human skin allografts

An outcome analysis and long-term viability of cryopreserved cultured epidermal allografts: assessment of the conservation of transplantable human skin allografts

Current insights into skin banking: storage, preservation and clinical importance of skin allografts

Skin graft storage in platelet rich plasma (PRP)

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