Quality Parameters and Antioxidant Activity of Three Clover Species in Relation to the Livestock Diet

Petrović, Mirjana; Stanković, Milan; Anđelković, B.; Babić, Snežana; Zornić, Vladimir; Vasiljević, Sanja; Dajić-Stevanović, Zora

doi:10.15835/nbha44110144

Cited by 14 publications

(8 citation statements)

References 23 publications

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“…lignans (Dai and Mumper, 2010). Various epidemiological studies have confirmed the anti-inflammatory, estrogenic, antimicrobial, antiallergenic and antitumor properties of the phenolic compounds in the Trifolium genus (Khorasani Esmaeili et al, 2015;Petrović et al, 2016). Isoflavones, the major phenolics present in the red clover, are categorized as phytoestrogens.…”

Section: Introductionmentioning

confidence: 99%

Phenolic Content and Antioxidant Activity of the Croatian Red Clover Germplasm Collection

et al. 2020

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The leaf samples of two varieties, twenty breeding populations, and seven local populations of the Croatian red clover were collected in the full flowering stage, with an aim to evaluate their phenolic content and antioxidant activity by using the spectrophotometric methods. A significant variation among the varieties/populations in the content of total phenolics and flavonoids was determined. The results demonstrated that the red clover is a rich source of phenolics and flavonoids, ranging from 38.67 to 59.96 mg GAE/g of DM and 21.19 to 51.48 mg CE/g of DM, respectively. The high levels in both phenolics and flavonoids were found in breeding populations/variety CD-8, Rc-11/7, Rc-11/8, Rc-11/15 and OS Osiris. The leaf extracts manifested a strong antioxidant activity towards diphenyl-1-picrylhydrazyl (DPPH), with a variation from 31.50 to 63.14%. A significant correlation was found between the antioxidant activity of the extracts and their total phenolic and flavonoid content (r = 0.737 and 0.839, respectively). Considering the obtained results, the crude extracts of red clover manifested a significant antioxidant potential and can be used as a natural source of biologically active components in the human and animal nutrition.

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Section: Introductionmentioning

confidence: 99%

Phenolic Content and Antioxidant Activity of the Croatian Red Clover Germplasm Collection

et al. 2020

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“…Biochanin A also shows a plenitude of biological activities such as hypoglycemic, anti-microbial, anti-cancer, anti-oxidant, as well as osteogenic and anti-inflammatory properties [ 14 , 15 ]. Other polyphenolic compounds, such as flavonoids, polyphenolic amides and phenolic acids, which are present in red clover, can also contribute to its antioxidant potential [ 7 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Composition of Different Red Clover Genotypes Based on Plant Part and Genetic Traits

Mikulić,

Atanacković Krstonošić,

Kladar

et al. 2023

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Red clover (Trifolium pratense L.) is an important legume that is also known as a rich source of isoflavones, which are compounds with mild estrogenic activity. Therefore, this plant is often used as a raw material in the production of dietary supplements recommended in menopause. Many factors can influence isoflavone content, but those genetically related are considered to be the most important. Therefore, the aim of this study was to evaluate the phytochemical profile of different plant parts of 30 red clover genotypes grouped according to ploidy and country of seed origin by analyzing the content of dominant isoflavones, total phenolic content (TPC) and antioxidant activity. It was found that there are significant differences in the examined traits among plant parts. Red clover leaves had the highest total isoflavone content, with biochanin A as the dominant compound, while flower extracts had the highest TPC and antioxidant activity. Diploid and tetraploid genotypes were significantly different concerning the content of daidzein, genistein, formononetin and TPC with higher quantities in tetraploid samples. On the other hand, seed origin was not a useful separating factor for the analyzed samples. The results of this research indicate that ploidy, as a previously poorly studied factor, could influence isoflavone content in red clover.

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“…There are numerous articles confirming antioxidant activity of Trifolium pratense L. The results of research suggested the great value of flowers and leaves of Trifolium species for their use in phytotherapy, due to their content of polyphenols responsible for the antioxidant activities; phenols in plants can also act as deterrents against herbivores, as antiviral and antimicrobial factors and photoprotectants against UV irradiation [ 19 , 20 , 21 ]. Using the right extraction method more phenolic compounds can be extracted, therefore, the antioxidant activity can be increased.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Traditional and Cyclodextrin-Assisted Extraction Methods on Trifolium pratense L. (Red Clover) Extracts Antioxidant Potential

et al. 2022

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Red clover is the subject of numerous studies because of its antioxidant properties, the positive influence of isoflavones on the health, and its potential use in the prevention and treatment of chronic diseases. The right excipients, such as cyclodextrins, can increase the profile of valuable phenolic compounds in extraction media to obtain rich in antioxidants, extracts that can be used in nutraceuticals production. The aim of this study was to investigate and compare the total phenolic content, flavonoid content, and antioxidant activity of red clover aerial parts, aqueous and ethanolic extracts prepared using traditional and cyclodextrins-assisted methods. The antioxidant activity of the extracts was established using ABTS, DPPH, FRAP, and ABTS-post column methods. It was determined that cyclodextrins significantly increased total phenolic content (compared with control)—using β-cyclodextrin 20.29% (in aqueous samples); γ-cyclodextrin 22.26% (in ethanolic samples). All the samples prepared with excipients demonstrated a strong relationship between total phenolic content and DPPH assay. Study showed that for extraction with water, the highest amounts of phenolic compounds, flavonoids and antioxidant activity will be achieved with β-cyclodextrin, but extractions with ethanol will give the best results with γ-cyclodextrin. Therefore, cyclodextrins are a great and safe tool for obtaining rich, red clover flower extracts that are high in antioxidant activity, which can be used in the pharmaceutical industry for nutraceuticals production.

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Quality Parameters and Antioxidant Activity of Three Clover Species in Relation to the Livestock Diet

Cited by 14 publications

References 23 publications

Phenolic Content and Antioxidant Activity of the Croatian Red Clover Germplasm Collection

Phenolic Content and Antioxidant Activity of the Croatian Red Clover Germplasm Collection

Phytochemical Composition of Different Red Clover Genotypes Based on Plant Part and Genetic Traits

The Effect of Traditional and Cyclodextrin-Assisted Extraction Methods on Trifolium pratense L. (Red Clover) Extracts Antioxidant Potential

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