Quality Related Safety Issue-Evidence-Based Validation of Herbal Medicine Farm to Pharma

Mukherjee, Pulok K.; Bahadur, Shiv; Chaudhary, Sushil Kumar; Kar, Amit; Mukherjee, Kakali

doi:10.1016/b978-0-12-800874-4.00001-5

Cited by 41 publications

(34 citation statements)

References 83 publications

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“…However, the lack of chemical markers remains a major problem in this context. 11 In 2012, Gantait et al 12 reported a validated HPTLC method for quantification of capsaicin in different varieties of capsicum found in India. 12 HPLC is one of the convenient and comprehensive technique for separating the individual components in plant extracts which has great importance in relation to their authentication, fingerprinting, quantification, quality control in herbal industry, 13 so we aimed to perform a comparative estimation of capsaicin through High Performance Liquid Chromatography (HPLC) in seven varieties of capsicum collected from different region of India.…”

Section: Introductionmentioning

confidence: 99%

Validation of Capsaicin in Indian Capsicum Species Through RP-HPLC

Nag¹,

Chanda²,

Biswas³

et al. 2017

IJPER

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Capsaicin is a pungent capsacinoid which differs in capsicum fruit within the species and the cultivars due to its topographical diversity. Here, a comparative estimation of capsaicin in seven varieties of capsicum has been performed by RP-HPLC method. The method was carried out in reverse phase C 18 column using acetonitrile and water (1% acetic acid) as mobile phase (65:35 v/v), at the flow rate of 1ml/min. The λmax was detected at 230 nm. The calibration curves were linear in the concentration range of 1-80 µg/ml. The comparative study revealed that the capsaicin content was highest (3.12% w/w) in Capsicum annuum Cayenne whereas Capsicum cardenasii contains the lowest (0.85% w/w). The % RSD of precision and recovery was found to be < 2%, which confirms high repeatability of the method. This method can be commercialized at industrial level for ensuring the highest quality of capsicum used as raw material.

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Section: Introductionmentioning

confidence: 99%

Validation of Capsaicin in Indian Capsicum Species Through RP-HPLC

Nag¹,

Chanda²,

Biswas³

et al. 2017

IJPER

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“…Furthermore, the quality control needs to measure the phytochemical compounds in medicinal plants for ensuring the quality reliability of natural products obtained from plant sources. [ 20 ] Thus, TLC fingerprint demonstrated the pattern of phytochemical characteristic constituents [ Figure 3 ].…”

Section: Resultsmentioning

confidence: 99%

Quality evaluation of Kaempferia parviflora rhizome with reference to 5,7-dimethoxyflavone

Pitakpawasutthi

Palanuvej

Ruangrungsi

2018

J Adv Pharm Technol Res

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Kaempferia parviflora Wall. ex Baker is a medicinal plant found in the upper Northeastern regions of Thailand, which belongs to Zingiberaceae family. The present study aims to investigate the standardization parameters, to analyze chemical constituents of volatile oil by gas chromatography-mass spectrometry, and to determine the content of 5,7-dimethoxyflavone in K. parviflora rhizomes by thin-layer chromatography (TLC)-densitometry compared to TLC image analysis. K. parviflora rhizomes from 15 different sources throughout Thailand were investigated for morphological and pharmacognostic parameters. 5,7-Dimethoxyflavone contents were determined by TLC-densitometry with winCATS software and TLC image analysis with ImageJ software. The mobile phase for TLC development consisted of toluene: chloroform: Acetone: formic acid (5: 4: 1: 0.2). For the Results, the pharmacognostic parameters of K. parviflora rhizome were demonstrated. The loss on drying, total ash, acid-insoluble ash, water content, volatile oil content, ethanol, and water-soluble extractive values were found to be 8.979 ± 0.041, 5.127 ± 0.060, 2.174 ± 0.092, 9.291 ± 0.458, 0.028 ± 0.003, 5.138 ± 0.092, and 8.254 ± 0.191 g/100 g of dry weight, respectively. K. parviflora volatile oil showed the major components of α-copaene, dauca-5, 8-diene, camphene, β-pinene, borneol, and linalool. The 5,7-dimethoxyflavone content of K. parviflora rhizomes determined by TLC-densitometry and TLC image analysis were found to be 2.15 ± 0.64 and 1.96 ± 0.51 g/100 g of dry rhizomes, respectively. The 5,7-dimethoxyflavone contents of both methods were not significantly different (P > 0.05) using paired t-test.

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“…The limited evidence of chemical marker remains a major problem for the quality control of herbal medicines. Keeping view, our present study dealt with the RP-HPLC method validation of aerial part of V. articulatum plant to ensure the content of active phytomolecule as well as reproducibility of the developed method [23] . The developed method was validated to quantify the amount of oleanolic acid in the aerial part of V. articulatum .…”

Section: Discussionmentioning

confidence: 99%

RP-HPLC analysis of methanol extract of Viscum articulatum

Nag

Kar

Chanda

et al. 2020

Journal of Ayurveda and Integrative Medicine

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Background Viscum articulatum Burm. (Family: Loranthaceae) is commonly known as mistletoe. In ayurveda, the plant parts are used in “Kapha”, “Vata”, diseases of the blood, ulcer, and epilepsy. The plant parts are also used in urinary tract infection and wound infection. The plant contains five triterpenoids such as α-amyrin, lupeol, betulin, betulinic acid and oleanolic acid, exhibiting several pharmacological activities including antimicrobial, anti-HIV, antitumor, antiviral activity. Objective To ensure the content of uniformity of oleanolic acid, a RP-HPLC method has been developed for estimation of oleanolic acid in V. articulatum aerial part. Material and methods The RP-HPLC method was carried out in reverse phase C 18 column, using methanol and water as mobile phase in the ratio of 95:5 (v/v), at the flow rate of 1 mL/min. The pH of aqueous phase was adjusted 3.2 with 1% (v/v) glacial acetic acid. The λ max was set at 210 nm. Results The retention time of oleanolic acid was found at 21.5 ± 0.05 min. The linearity of the response was found to be 10–800 μg/mL. The coefficient of determinants of oleanolic acid was found to be (r 2 ) 0.995 and equation Y = 19462X + 16,172. The LOD and LOQ were found to be for oleanolic acid (1.96% w/w) 0.197 ± 0.63 and 0.623 ± 0.87 μg/mL, respectively. The developed method was accurate, specific, precise and reproducible. Conclusion This RP-HPLC may be useful for quantitative estimation of the chemical constituents present in the plant extract as well as the quality assessment of the herbal product.

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Quality Related Safety Issue-Evidence-Based Validation of Herbal Medicine Farm to Pharma

Cited by 41 publications

References 83 publications

Validation of Capsaicin in Indian Capsicum Species Through RP-HPLC

Validation of Capsaicin in Indian Capsicum Species Through RP-HPLC

Quality evaluation of Kaempferia parviflora rhizome with reference to 5,7-dimethoxyflavone

RP-HPLC analysis of methanol extract of Viscum articulatum

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