Quantification and expression studies of IL-13 gene in response to experimental infection with Haemonchus contortus in Garole and Sahabadi sheep

Patra, Gautam; Borthakur, Sonjoy Kumar; Das, Samares Kumar; Lalliankimi, Hmar; Lalrinkima, H.

doi:10.1016/j.vprsr.2017.03.002

Cited by 2 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the differentially expressed genes, upregulation of the IL13 gene in H. contortus-susceptible Morada Nova is in accordance with a previous study using infected young sheep of a native breed [69]. However, IL13 was also detected as upregulated in adult resistant sheep [70]. In addition to animal age, the infection length should be considered, as genes related to the in ammatory response, T lymphocyte attraction, and leukocyte binding are upregulated in the abomasal lymph nodes of resistant animals at the beginning of T. circumcincta infection but downregulated at later stages, evidencing a delayed Th2 response in susceptible animals [71].…”

Section: Discussionsupporting

confidence: 89%

“…In addition to animal age, the infection length should be considered, as genes related to the in ammatory response, T lymphocyte attraction, and leukocyte binding are upregulated in the abomasal lymph nodes of resistant animals at the beginning of T. circumcincta infection but downregulated at later stages, evidencing a delayed Th2 response in susceptible animals [71]. The roles of IL13 in resistance to H. contortus are through smooth muscle hypercontraction and increased mucus production, resulting in detachment of parasites from the gut wall [70].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Multi-omics elucidated parasite-host-microbiota interactions and resistance to Haemonchus contortus in sheep

Niciura,

Cardoso,

Ibelli

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: The integration of molecular data from hosts, parasites, and microbiota can enhance our understanding of the complex biological interactions underlying parasite resistance. Haemonchus contortus, the predominant sheep parasite species in the tropics, results in significant production and economic losses, which are further compounded by the diminishing efficiency of chemical control measures due to anthelmintic resistance. Knowledge of how the host responds to infection and how the parasite, in combination with microbiota effects, modulates host immunity can guide selection decisions to breed more resistant animals. This understanding can also refine management practices and inform the development of new therapeutics for long-term helminth control. Results: Egg per gram (EPG) counts were obtained in Morada Nova sheep subjected to two artificial infections with H. contortus, and used as a proxy to select animals with high resistance or susceptibility. The GAST, GNLY, IL13, MGRN1, FGF14, and RORC genes and transcripts were differentially expressed between groups based on RNA-seq of the abomasum. From 50K SNP genotyping, EPG heritability estimate was 0.12, and a genome-wide association study (GWAS) identified regions on chromosomes 2 and 11 harboring candidate genes for resistance, immune response, body weight, and adaptation. Trans-eQTLs between significant variants and differentially expressed transcripts were found. Amplicon sequence variants (ASVs) from PCR amplification and sequencing of bacterial and archaeal 16S rRNA genes in sheep feces and rumen generated functional co-expression modules correlated with resistance to H. contortus, showing enrichment in pathways of response to bacterium, immune and inflammatory responses, and hub features of the Christensenellaceae, Bacteroides, and Methanobrevibacter genera, Prevotellaceae family, and Verrucomicrobiota. In RNA-seq of H. contortus, some mitochondrial, collagen- and cuticle-related genes were expressed only in parasites retrieved from susceptible sheep. Conclusions: This study identified chromosome regions, genes, transcripts, and pathways involved in the elaborate interactions between the sheep host, its gastrointestinal microbiota and the H. contortus parasite. These findings can assist with the development of animal selection strategies for parasite resistance and interdisciplinary approaches to control H. contortus in sheep.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Multi-omics elucidated parasite-host-microbiota interactions and resistance to Haemonchus contortus in sheep

Niciura,

Cardoso,

Ibelli

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Genetic resistance to gastrointestinal parasites in sheep

et al. 2023

View full text Add to dashboard Cite

Gastrointestinal nematode (GIN) infection is the most common disease affecting sheep production systems throughout the world, causing significant productive and economic losses. The control of these parasites was traditionally based on the use of anthelmintic drugs. However, continuous, extensive, and indiscriminate use of these drugs has led to the emergence and spread of strains of parasites resistant to the major chemical compounds used. This situation has led to considering alternative strategies for worm control. One of them is the selection of individuals for greater resistance to GINs. A number of phenotypic traits have been reported in the bibliography, including parasitological, biochemical, hematological, and immunological traits, but fecal egg count (FEC) is considered the primary and most practical measure of resistance. Genomic studies have reported polymorphisms associated with GIN resistance traits on almost all sheep chromosomes. Among other loci, FEC has been associated with the major histocompatibility complex (MHC) through quantitative trait loci (QTL) analysis and genome-wide association studies (GWAS). This review covers the principal aspects reported in the literature on several parameters considered to evaluate the resistance status of sheep to nematodes, mainly Haemonchus contortus , the correlation between resistance markers with economically important production traits, and the main genomic regions identified as relevant in determining the phenotype for resistance or susceptibility to GINs.

show abstract

Quantification and expression studies of IL-13 gene in response to experimental infection with Haemonchus contortus in Garole and Sahabadi sheep

Cited by 2 publications

References 22 publications

Multi-omics elucidated parasite-host-microbiota interactions and resistance to Haemonchus contortus in sheep

Multi-omics elucidated parasite-host-microbiota interactions and resistance to Haemonchus contortus in sheep

Genetic resistance to gastrointestinal parasites in sheep

Contact Info

Product

Resources

About