Quantification and visualization of cellular NAD(P)H in young and aged female facial skin with<i>in vivo</i>two-photon tomography

Miyamoto, Kukizo; Kudoh, Hitoshi

doi:10.1111/bjd.12370

Cited by 33 publications

(31 citation statements)

References 35 publications

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“…The certified clinical tomograph MPTflex as a mobile flexible nonlinear imaging system simplifies in vivo AF/SHG imaging of human skin [21][22][23][24] . An articulated optical mirror-arm with active beam position control for free-space-beam delivery between laser and scan head allows a high degree of freedom for positioning of the scan head which is inevitable for a variety of medical applications (Figure 1a).…”

Section: Methodsmentioning

confidence: 99%

A novel clinical multimodal multiphoton tomograph for AF, SHG, CARS imaging, and FLIM

2014

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We report on a flexible nonlinear medical tomograph with multiple miniaturized detectors for simultaneous acquisition of two-photon autofluorescence (AF), second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) images. The simultaneous visualization of the distribution of endogenous fluorophores NAD(P)H, melanin and elastin, SHG-active collagen and as well as non-fluorescent lipids within human skin in vivo is possible. Furthermore, fluorescence lifetime images (FLIM) can be generated using time-correlated single photon counting.

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Section: Methodsmentioning

confidence: 99%

A novel clinical multimodal multiphoton tomograph for AF, SHG, CARS imaging, and FLIM

2014

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“…Research models for melanization and dermal processes such as collagen renewal or organization (whitening, anti-ageing) [90][91][92] Can apply to all skin diseases. Real-time non-invasive 'biopsies', malignant processes [93][94][95] 38,39 QoL and skin diseases.…”

Section: Developments In the Evaluation Of Cosmetic Formulationsmentioning

confidence: 99%

The science of dermocosmetics and its role in dermatology

Dréno

Araviiskaia

Berardesca

et al. 2014

Acad Dermatol Venereol

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Our increased knowledge of normal skin physiology has ushered in a subtle revolution in cosmetic science. Originally designed as preparations to enhance personal appearance by direct application on to the skin, cosmetics have now taken on a new role in dermatology, through the support of the management of many skin disorders. This evolving role of cosmetics in skin care is primarily due to scientific and technological advancements that have changed our understanding of normal skin physiology and how cosmetics modify its appearance both physically and biologically. The vast array of techniques currently available to investigate skin responsivity to multiple stimuli has brought about a new era in cosmetic and dermocosmetic development based on a robust understanding of skin physiology and its varied responses to commonly encountered environmental insults. Most cosmetic research is undertaken on reconstructed skin models crucial in dermatological research, given the strict ban imposed by the European Union on animal testing. In addition, the design and conduct of trials evaluating cosmetics now follow rules comparable to those used in the development and evaluation of pharmaceutical products. Cosmetic research should now aim to ensure all trials adhere to strictly reproducible and scientifically sound methodologies. The objective of this review is to provide an overview of the multidisciplinary scientific approach used in formulating dermocosmetics, and to examine the major advances in dermocosmetic development and assessment, the safety and regulatory guidelines governing their production and the exciting future outlook for these dermocosmetic processes following good practice rules.

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“…During the last decade, "CE marked" AF/SHG based tomographs have been developed including systems with an additional CARS option. [10][11][12]20 The certified clinical AF/SHG-CARS tomograph MPTflex CARS (JenLab, Germany) as a mobile flexible nonlinear imaging system not only simplifies in vivo AF/SHG imaging of human skin 9,[24][25][26] but is also a multipurpose system which can be used for small animal research, in vivo stem cell tracking 27 and ex vivo biopsy imaging. Free-space-beam delivery inside an articulated optical mirror arm is provided by active beam position control.…”

Section: Introductionmentioning

confidence: 99%

Multipurpose nonlinear optical imaging system forin vivoandex vivomultimodal histology

et al. 2015

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Abstract. We report on a flexible multipurpose nonlinear microscopic imaging system based on a femtosecond excitation source and a photonic crystal fiber with multiple miniaturized time-correlated single-photon counting detectors. The system provides the simultaneous acquisition of e.g., two-photon autofluorescence, secondharmonic generation, and coherent anti-Stokes Raman scattering images. Its flexible scan head permits ex vivo biological imaging with subcellular resolution such as rapid biopsy examination during surgery as well as imaging on small as well as large animals. Above all, such an arrangement perfectly matches the needs for the clinical investigation of human skin in vivo where knowledge about the distribution of endogenous fluorophores, second-harmonic generation-active collagen as well as nonfluorescent lipids is of high interest.

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Quantification and visualization of cellular NAD(P)H in young and aged female facial skin within vivotwo-photon tomography

Cited by 33 publications

References 35 publications

A novel clinical multimodal multiphoton tomograph for AF, SHG, CARS imaging, and FLIM

A novel clinical multimodal multiphoton tomograph for AF, SHG, CARS imaging, and FLIM

The science of dermocosmetics and its role in dermatology

Multipurpose nonlinear optical imaging system forin vivoandex vivomultimodal histology

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