Quantification of 37 glucocorticoids in chicken muscle by UHPLC-Q-Orbitrap-MS with parallel reaction monitoring

Pan, Xiaodong; Han, Jiaguang; Xu, Xiaoming

doi:10.1039/d3ay00830d

Cited by 5 publications

References 28 publications

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Untargeted Profiling of Shenfu Qiangxin Pills Based on High‐Resolution Mass Spectrometry and Absolute Quantitation of Multiple Components Using Parallel Reaction Monitoring

Gao,

Liang,

Zhang

et al. 2024

J of Separation Science

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Shenfu Qiangxin (SFQX) pills are proprietary traditional Chinese medicine used for the treatment of chronic heart failure with significant clinical effects. However, the systematic identification and quantification of complexed components in SFQX have not been reported yet. In this work, a reliable and comprehensive method for a rapid identification of chemical components was developed by data dependent acquisition using ultra performance liquid chromatography coupled with high‐resolution mass spectrometry (UHPLC‐Q Exactive‐Orbitrap‐MS). A total of 104 compounds mainly including flavonoids, saponins, alkaloids, anthraquinones, coumarins, and phenolic acids were identified through database searching. The identified compounds were further ascribed to herb species in SFQX pills, such as Ginseng Radis et Rhizoma, Aconiti Lateralis Radix Praeparata (processed), Mori Cortex, Lepidii Semen, Rhei Radix et Rhizoma, and Polyporus. Thirty‐two representative compounds were elaborated with cleavage pathways and characteristic fragments in MS/MS spectrum of commercially available standards. A quantification method based on parallel reaction monitoring technique was established, and absolute quantification of 28 components in SFQX pills was then carried out. This work constitutes a basis and methodological reference for the quality control, consistency evaluation, and standardization of similar proprietary Chinese medicines.

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Untargeted Profiling of Shenfu Qiangxin Pills Based on High‐Resolution Mass Spectrometry and Absolute Quantitation of Multiple Components Using Parallel Reaction Monitoring

Gao,

Liang,

Zhang

et al. 2024

J of Separation Science

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Antibiotic residues in commercial freshwater fish from southeast China: distribution and human health risk assessment

Shao,

Pan,

Han

2024

Environ Sci Pollut Res

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We investigated 14 antibiotic residues in 8 marketed freshwater sh species from southeast China and estimated the associated health risks to local consumers. The antibiotic residues were determined by UPLC-MS/MS. Our ndings revealed widespread distribution of quinolones (QNs), tetracyclines (TCs), and chloramphenicols (CAPs) in the freshwater sh. Notably, the average concentrations of enro oxacin and cipro oxacin reached levels as high as 62.5 µg/kg wet weight (ww) and 11.7 µg/kg ww, respectively, and detection frequencies were 68.7% for enro oxacin and 31.6% for cipro oxacin. Additionally, we detected chloramphenicol, a prohibited antibiotic, in samples with a detection frequency of 0.76%. Among the sh species, the mean concentration of total antibiotic residues was highest in bluntnose black bream (263.3 µg/kg), followed by English perch (52.4 µg/kg), crucian carp (46.3 µg/kg), black carp (28.6 µg/kg), yellowcheek carp (21.0 µg/kg), grass carp (15.3 µg/kg), bighead carp (3.78 µg/kg), and mandarin sh (3.69 µg/kg). We estimated the daily intake values of these antibiotic residues which were lower than the acceptable daily intake values and hazard indexes were much less than 1. It indicates there is very low direct health risk to consumers. Despite that, investigation on the chronic impact, such as antibiotic-resistant bacteria, gut microbiota disruption and allergic reactions is urgently needed.

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Occurrence and risk assessment of coccidiostat residues in chicken meat: A regional study in Southeast China

Shen,

Pan,

Han

2024

Food Bioscience

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Quantification of 37 glucocorticoids in chicken muscle by UHPLC-Q-Orbitrap-MS with parallel reaction monitoring

Abstract: The quantification capability of high-resolution mass spectrometry (HRMS) has received increasing concern among analysts. In this study, we present a method for analyzing 37 glucocorticoids in chicken muscle using UHPLC-Q-Orbitrap...

Cited by 5 publications

References 28 publications

Untargeted Profiling of Shenfu Qiangxin Pills Based on High‐Resolution Mass Spectrometry and Absolute Quantitation of Multiple Components Using Parallel Reaction Monitoring

Untargeted Profiling of Shenfu Qiangxin Pills Based on High‐Resolution Mass Spectrometry and Absolute Quantitation of Multiple Components Using Parallel Reaction Monitoring

Antibiotic residues in commercial freshwater fish from southeast China: distribution and human health risk assessment

Occurrence and risk assessment of coccidiostat residues in chicken meat: A regional study in Southeast China

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