Quantification of a glucocorticoid receptor in sea bass (Dicentrarchus labrax, L.) reared at high stocking density

Terova, Genciana; Gornati, Rosalba; Rimoldi, Simona; Bernardini, Giovanni; Saroglia, Marco

doi:10.1016/j.gene.2005.06.016

Cited by 106 publications

(66 citation statements)

References 37 publications

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“…GR gene duplicate copies (DlGR1 and DlGR2) that have been found in sea bass (Vizzini et al, 2007;Terova et al, 2005) are in accordance to the GR gene duplication within the teleost lineage. We compared the deduced amino acid sequences of D. labrax (Perciformes, Moronidae) DlGR1 (Vizzini et al, 2007) and DlGR2 (indicated as GR by Terova et al, 2005), and both were compared to HbGR1, HbGR2a and HbGR2b isoforms of H. burtoni (Perciformes, Cichlidae) (Greenwood et al, 2003).…”

Section: Discussionsupporting

confidence: 61%

“…In sea bass (Dicentrarchus labrax), two distinct GRs have been sequenced: the DlGR1 from the peritoneal cavity cells (Vizzini et al, 2007) and a GR (here named DlGR2) from the liver (Terova et al, 2005). DlGR1 cDNA disclosed four functional domains, for transcriptional activation, DNA-binding, nuclear localization, and hormonebinding; it is expressed in macrophages and neutrophils from the peritoneal cavity (Vizzini et al, 2007), in the head kidney, spleen, gill, intestine, heart and liver tissues (Vazzana et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…DlGR1 cDNA disclosed four functional domains, for transcriptional activation, DNA-binding, nuclear localization, and hormonebinding; it is expressed in macrophages and neutrophils from the peritoneal cavity (Vizzini et al, 2007), in the head kidney, spleen, gill, intestine, heart and liver tissues (Vazzana et al, 2008). Although stress-associated increased plasma cortisol can modify the GR gene expression, contrasting results on gene regulation have been reported (Terova et al, 2005;Vijayan et al, 2003;Sathiyaa and Vijayan, 2003;Takahashi et al, 2006). In the present paper, DlGR1, DlGR2 and HbGR sequences were compared and homology levels disclosed.…”

Section: Introductionmentioning

confidence: 99%

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Differential expression of two glucocorticoid receptors in seabass (teleost fish) head kidney after exogeneous cortisol inoculation

Vazzana

Vizzini

Sanfratello

et al. 2010

Comparative Biochemistry and Physiology Part A: Molecular &

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Stressful conditions include a prompt release of corticosteroid hormones which can mediate gene expression through glucocorticoid receptors (GR). Since two seabass (Dicentrarchus labrax) GRs have been cloned and sequenced from peritoneal cavity cells (DlGR1) and liver (DlGR2), a comparative amino acid sequence analysis that included Haplochromis burtoni HbGRs, was carried out and homologies disclosed. The DlGR1 and DlGR2 deduced aminoacid sequences showed 61% identity (I) and 70% similarity (S). Moreover, DlGR2 was similar to HbGR2b (69% I, 73% S), and the DlGR1 to HbGR1 (72% I, 78% S). In addition, we examined the expression of the DlGRs after exogeneous cortisol inoculation into the peritoneal cavity, mimicking stress effects. At various times after the administration (3 h, 24 h, 1 week), gene expressions was evaluated in head kidney by real-time PCR. In addition, immunoblotting and densitometry analyses were performed with antiDlGR1 antibodies. Although sea bass head kidney expressed both DlGR1 and DlGR2 they were differentially modulated by intraperitoneal implant of exogeneous cortisol.

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Section: Discussionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differential expression of two glucocorticoid receptors in seabass (teleost fish) head kidney after exogeneous cortisol inoculation

Vazzana

Vizzini

Sanfratello

et al. 2010

Comparative Biochemistry and Physiology Part A: Molecular &

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“…Such scientific indicators vary from the measurement of cortisol excreted into water (reviewed in Ellis 2007 andScott et al 2008) into more complex approaches such as the upand down-regulation of targeted genes (e.g. Terova et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Behavioural indicators of welfare in farmed fish

Martins¹,

Galhardo²,

Noble³

et al. 2011

Current Views on Fish Welfare

120

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Behaviour represents a reaction to the environment as fish perceive it and is therefore a key element of fish welfare. This review summarises the main findings on how behavioural changes have been used to assess welfare in farmed fish, using both functional and feeling-based approaches. Changes in foraging behaviour, ventilatory activity, aggression, individual and group swimming behaviour, stereotypic and abnormal behaviour have been linked with acute and chronic stressors in aquaculture and can therefore be regarded as likely indicators of poor welfare. On the contrary, measurements of exploratory behaviour, feed anticipatory activity and rewardrelated operant behaviour are beginning to be considered as indicators of positive emotions and welfare in

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“…No significant differences were found in the haematocrit values, or in hepatosomatic indices in the groups of fish held at any of the stocking densities. Terova et al (2005) demonstrated that a high rearing density (80 and 100 kg m -3 ) of sea bass affects the glucocorticoid receptor mRNA, the abundance of which in the liver decreased inversely with blood cortisol levels. Montero et al (1999) found that a high stocking density (40 kg m -3 ) produces crowding stress that alters some physiological and biochemical parameters in sea bream juveniles.…”

Section: Discussionmentioning

confidence: 99%

The effect of organic and conventional production methods on sea bream growth, health and body composition: a field experiment

Mente¹,

Stratakos²,

Boziaris³

et al. 2012

Sci. Mar.

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SUMMARY: This study aimed to develop a better understanding of organic sea bream aquaculture production in Greece, in particular its consequences for fish growth, health and body composition, and to propose and update standards for sustainable organic sea bream farming. Gilthead sea bream were kept in sea cages at densities of 4 kg m -3 (organic) and 15 kg m -3 (conventional), and were fed organically produced feed (45% crude protein, 14% fat) or conventional feed (46% crude protein, 17% fat). The amino acid profile of the conventional diet, particularly the lysine content, which is one of most important dietary amino acids for sea bream, appeared to be unsatisfactory. "Organic" sea bream stored less fat content in their white muscle than the conventional sea bream. The liver lipid content was lower and the hepatosomatic index was higher for the organic sea bream. The microbiological analysis showed that both Enterobacteriaceae and Escherichia coli on the skin were below the enumeration detection limit in both the organic and conventional sea bream. Total viable counts on the skin and muscle of both the organically and conventionally cultured sea bream were approximately 3 log cfu g -1 , which is well below the acceptable limit (7 log cfu g -1 ) for marine species. The results showed that the combination of a low stocking density and feed with a different ingredient composition but similar nutritional value resulted in similar growth rates and nutrient profiles of the final product. Further research on nutrition is required to provide information on setting the appropriate standards for organic sea bream aquaculture to ensure that the final product is in line with the consumers' preferences.Keywords: sustainable, organic, sea bream aquaculture. RESUMEN: EfEcto dE los métodos dE producción orgánica y convEncional En El crEcimiEnto, salud y composición corporal dEl pargo: un ExpErimEnto dE campo. -Este estudio tiene por objeto desarrollar una mejor comprensión de la producción acuícola orgánica del pargo en Grecia, en particular sus consecuencias en relación al crecimiento del pez, su salud y su composición corporal, y proponer y actualizar los estándares para la cría sostenible y orgánica. Los pargos dorados fueron criados en contenedores con densidades de 4 kg m -3 (orgánico) y de 15 kg m -3 (convencional) y les fue ofrecido alimento producido orgánicamente (45% de proteina cruda, 14% de grasa) y convencionalmente (46% de proteina cruda, 17% de grasa). El perfil de los aminoácidos de la dieta convencional, en especial en relación al contenido en lisina, uno de los aminoácidos alimentarios más importantes para pargos, pareció ser insatisfactorio. Los pargos "orgánicos" acumularon una menor cantidad de grasa en sus músculos blancos en compración con los pargos convencionales; el contenido en lípidos del hígado es menor y el índice hepatosomático es más alto en los pargos orgánicos. Los análisis microbiológicos muestran que las Enterobacteriaceae y las Escherichia coli en la piel estaban por debajo del límite de de...

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Quantification of a glucocorticoid receptor in sea bass (Dicentrarchus labrax, L.) reared at high stocking density

Cited by 106 publications

References 37 publications

Differential expression of two glucocorticoid receptors in seabass (teleost fish) head kidney after exogeneous cortisol inoculation

Differential expression of two glucocorticoid receptors in seabass (teleost fish) head kidney after exogeneous cortisol inoculation

Behavioural indicators of welfare in farmed fish

The effect of organic and conventional production methods on sea bream growth, health and body composition: a field experiment

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