Quantification of anammox activity in a denitrification reactor for a recirculating aquaculture system

The diversity and distribution of anammox bacteria in the marine aquaculture zones

“…The differences of anammox bacteria community structures of the present study and the previous ones are possibly due to the difference in aquaculture systems, where previous reports were related to the recirculating aquaculture of freshwater and marine systems (Tal et al 2006, van Kessel et al 2010, van Kessel et al 2011, Castine et al 2012). However, some results of the current ones were quite similar to those of previous studies (Egli et al 2001, Tal et al 2006, Lahav et al 2009, van Kessel et al 2010, like the novel Planctomycete-like bacterial 16S rRNA gene sequences that were detected in two marine aquaculture zones with relatively high diversity, indicating that these new Planctomycete-like bacteria are very common in aquaculture systems. These unknown bacterial sequences share 83-86 % sequence identity to known anammox members, but their activity and ecophysiology still need to be confirmed in future studies.…”

“…Since their discovery in 1990s, the anammox bacteria, affiliated to the Planctomycetes (Strous et al 1999), have been detected in wastewater treatment plants and in various natural ecosystems, including marine (Thamdrup and Dalsgaard 2002, Dalsgaard et al 2003, Kuypers et al 2003, Kuypers et al 2005a, Kuypers et al 2005b, Hamersley et al 2007, Lam et al 2009, Ward et al 2009, Li and Gu 2013, Castro-Gonzalez et al 2014, freshwater (Penton et al 2006, Schubert et al 2006, Zhang et al 2007, Lee et al 2014, Smith et al 2015, terrestrial (Humbert et al 2010, Wang and, extreme (Byrne et al 2009, Li et al 2010a, Zhu et al 2015, intestinal tracts of fish (Chan et al 2016), and polychaetes (Li and Gu 2016). Several studies have also reported the activity and biodiversity of anammox bacteria in aquaculture systems, such as the biofilters in freshwater recirculating aquaculture systems (Tal et al 2006, Lahav et al 2009, van Kessel et al 2010, van Kessel et al 2011, Castine et al 2012), a denitrification reactor for a recirculating aquaculture system (Lahav et al 2009), and sediments of the shrimp ponds (Amano et al 2011) and marine aquaculture zones (Li et al 2010b, Li et al 2011b. However, researches about anammox bacteria diversity and distribution in marine aquaculture systems are still very limited, and more efforts should be taken in order to comprehensively describe this important N removal process in aquaculture systems.…”

End-of-pipe denitrification using RAS effluent waste streams: Effect of C/N-ratio and hydraulic retention time

“…However, since the MTF sludge storage basin was not operated as a completely mixed system, it had been continuously in use (> 2 years), and it was the source feeding organic matter and biomass into the denitrifying reactors, it seems reasonable to assume that such bacteria existed in the environment. Anammox bacteria have been identified in (marine) seabream RAS environment (solid waste, faeces, and fish intestines) (Lahav et al, 2009), and the contribution of the anammox process in denitrifying filters has previously been quantified to 15% of the total removal .…”

Denitrification and Dissimilatory Nitrate Reduction to Ammonium (DNRA) Activities in Freshwater Sludge and Biofloc from Nile Tilapia Aquaculture Systems

“…These POMs are removed in a separate compartment and degraded through anaerobic decomposition. Denitrifying reactors supplied with digested sludge have been found to operate successfully without the need for external carbon addition (Aboutboul et al, 1995;Klas et al, 2006;Tal et al, 2009). Hence, this is the method of choice when it comes to nitrate removal in aquaculture systems.…”

“…Favorable conditions for these pathways are hypothesized to depend mainly on the concentration and type of electron donor, the amount of inorganic nitrogen compounds present, along with the redox potential found in those systems (van Rijn et al, 2006;Kraft et al, 2011). To date, there had been several reports on the presence of anammox bacteria in aquaculture treatment systems that use degraded sludge as the substrates for denitrification (Tal et al, 2006;Lahav et al, 2009). However, the involvement of the DNRA process in aquaculture systems is still uncertain.…”