Quantification of cellular adhesion molecules on malignant B cells from non-Hodgkin’s lymphoma

Jacob, Marie‐Christine; Agrawal, Samir; Chaperot, Laurence; Giroux, C; Gressin, Rémy; Marc’hadour, F. Le; Favre, Mireille; Sotto, Jean‐Jacques; Bensa, Jean‐Claude; Plumas, Joël

doi:10.1038/sj.leu.2401517

Cited by 19 publications

(14 citation statements)

References 24 publications

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“…Quantification using flow cytometry has rarely been reported in the literature but it has been recently proved to be effective in the differential diagnosis of non-Hodgkin's lymphomas. 16 In our experience, the results of MCL immunophenotype were not dependent upon sample origin, bone marrow, peripheral blood or lymph node. Therefore, the results obtained in all MCL samples were globally compared to those obtained in mixed/atypical CLL cells.…”

Section: Table 4bmentioning

confidence: 50%

“…17 Several adhesion molecules, like CD54, CD11a/CD18, CD29/CD49d have been implicated in adhesion of B lymphocytes to stromal Leukemia cells: fibroblasts 18 or follicular dendritic cells. [19][20][21] The results obtained with CD11a and CD54 by Jacob et al 16 suggest that in low grade NHL, malignant cells remain localised because they are able to adhere to neighbouring cells. In the present work, CD54 and CD11a expression was not different between lymph node and peripheral blood and bone marrow, suggesting that, at least in this small group, disseminating cells had the same immunophenotype as tumour cells.…”

Section: Table 4bmentioning

confidence: 98%

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Atypical lymphocytic leukemia and mantle cell lymphoma immunologically very close: flow cytometric distinction by the use of CD20 and CD54 expression

et al. 2001

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Integration of morphological and immunophenotypic data is critical in achieving diagnosis accuracy and minimising interobserver interpretative discrepancies. The aim of this work was to compare the immunophenotype and the morphology of chronic lymphocytic leukaemia and mantle cell lymphoma, to help in the differential diagnosis of CD5 positive monoclonal B cells. Frozen/thawed samples from 91 patients were analysed retrospectively. Fresh samples from 17 mixed/atypical CLL and 13 MCL were tested to corroborate the results. Markers were analysed as percentage (%) of positive B lymphocyte subpopulation, and in terms of median fluorescence intensity (MFI). Matutes's CLL score clearly allowed distinguishing between classical CLL on the one hand, and atypical CLL and MCL on the other hand. The percentage of CD54-positive cells and the median fluorescence intensity of CD20 and CD54 were the only parameters which were significantly higher in MCL than in atypical CLL (P Ͻ 0.05), allowing an immunological distinction between these two entities. Nevertheless, due to a quenching problem when using CD20 and CD54 together, and because CD18 showed a statistically different expression between classical and atypical CLL, the combination of CD18/CD54 has been preferred and showed a different pattern in the three entities. Immunophenotyping could be helpful in the differential diagnosis of CD5-positive B cell chronic lymphoproliferative disorders with atypical features that do not fit exactly into any of the morphologic proposed groups. Leukemia (2001) 15, 1458-1465.

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Section: Table 4bmentioning

confidence: 50%

Section: Table 4bmentioning

confidence: 98%

Atypical lymphocytic leukemia and mantle cell lymphoma immunologically very close: flow cytometric distinction by the use of CD20 and CD54 expression

et al. 2001

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“…However, none of our six patients diagnosed with typical MCL presented with signs to suspect such an event and, in addition, three of them showed lymphoid cells of typical MCL in the CSF. On the other hand, it is well known that cellular adhesion molecules (CAM) are involved in the pattern of growth and dissemination of lymphoproliferative disorders [26][27][28][29][30]. MCL is characterized by low levels or absence of L-selectin and CD11c, low expression of CD11a/CD18 (Leukocyte function antigen (LFA)-1), and high levels of CD44, CD54 (Intracellular adhesion molecule-1), and VLA-5 [31,32].…”

Section: Discussionmentioning

confidence: 99%

Central nervous system involvement in mantle cell lymphoma

Ferrer¹,

Bosch²,

Villamor³

et al. 2008

Annals of Oncology

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Background: Extranodal involvement, including central nervous system (CNS), is a frequent event in patients with mantle cell lymphoma (MCL). However, the incidence, risk factors, and impact on outcome remain controversial. Patients and methods:Main clinical, biological, and evolutive features of 82 patients (60 males/22 females; median age: 61 years) diagnosed with MCL (blastoid, 26%) in a single institution were analyzed for risk of CNS involvement and prognosis. Results: Most patients had advanced stage and intermediate or high-risk International Prognostic Index (IPI). Elevenpatients eventually developed CNS involvement with an actuarial 5-year risk of 26% (95% confidence interval 10% to 42%). In one asymptomatic patient, cerebrospinal fluid infiltration was detected at staging maneuvers (1/62; 1.6%). The remaining 10 patients developed neurological symptoms during the course of the disease (median time from diagnosis, 25 months). Initial variables predicting CNS involvement were blastoid histology, high proliferative index measured by Ki-67 staining, high lactate dehydrogenase (LDH) and intermediate-or high-risk IPI. Histological subtype and serum LDH maintained significance in multivariate analysis. Treatment of CNS infiltration consisted of intrathecal chemotherapy (two cases), and intrathecal chemotherapy plus systemic treatment (seven cases). Median survival after CNS involvement was 4.8 months, patients with this complication having shorter survival than those with no CNS disease.Conclusion: This study confirms the high incidence of CNS involvement in MCL patients. Treatments aimed at preventing this complication are warranted.

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“…CD58 is expressed on all cell types 13 and at high levels on aggressive diffuse large B-cell lymphoma, 14 whereas it is also frequently over expressed in B-cell acute lymphoblastic leukaemia. 15 Together these observations suggest that CAR T cells targeting these malignancies may benefit from CD2-based signalling and enhanced-IL-2 production.…”

Section: Resultsmentioning

confidence: 99%

Ligation of the CD2 co-stimulatory receptor enhances IL-2 production from first-generation chimeric antigen receptor T cells

et al. 2011

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T cells bearing chimeric antigen receptors (CARs) are broadly categorised into first-and second-generation receptors. Second-generation CARs contain a co-stimulatory signalling molecule and have been shown to secrete IL-2, undergo greater proliferation and to have enhanced persistence in vivo. However, we have shown that T cells bearing a first-generation CAR containing a CD19-targeting scFv (single-chain variable fragment) and the CD3z-signalling domain are able to produce IL-2 upon co-culture with CD19 þ B-cell lymphomas independent of CD28 activity. Here, we report that signalling through endogenous CD2 following ligation with its ligands, CD48 in mouse and CD58 in humans, drives IL-2 production by firstgeneration CD19-specific CAR. Moreover, the high levels of IL-2 produced by human T cells engrafted with a second-generation CD28-containing CAR during target-cell recognition are dependent to a degree upon CD2 receptor activity. These observations highlight the fact that the functional activity induced by T-cell-expressed CARs is dependent upon endogenous 'natural' receptor interactions. A deeper understanding of the role of these activities will serve to further refine the design of future CARs to either exploit or avoid these interactions.

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Quantification of cellular adhesion molecules on malignant B cells from non-Hodgkin’s lymphoma

Cited by 19 publications

References 24 publications

Atypical lymphocytic leukemia and mantle cell lymphoma immunologically very close: flow cytometric distinction by the use of CD20 and CD54 expression

Atypical lymphocytic leukemia and mantle cell lymphoma immunologically very close: flow cytometric distinction by the use of CD20 and CD54 expression

Central nervous system involvement in mantle cell lymphoma

Ligation of the CD2 co-stimulatory receptor enhances IL-2 production from first-generation chimeric antigen receptor T cells

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