Quantification of hCG Hormone Using Tapered Optical Fiber Decorated with Gold Nanoparticles

Abstract

In this study, a novel technique for the quantification of the human chorionic gonadotropin (hCG) hormone using localized surface plasmons and a tapered optical fiber decorated with gold nanoparticles (Au-NPs) is reported. The tapered optical fiber fabrication process involves stretching a single-mode optical fiber using the flame-brushing system. The waist of the tapered optical fiber reaches a diameter of 3 μm. Decoration of the taper is achieved through the photodeposition of 100 nm Au-NPs using the drop-casting technique and a radiation source emitting at 1550 nm. The presence of the hCG hormone in the sample solutions is verified by Fourier-transform infrared spectroscopy (FTIR), revealing the presence of bands related to functional groups, such as C=O (1630 cm−1), which are associated with proteins and lipids, components of the hCG hormone. Quantification tests for hormone concentrations were carried out by measuring the optical power response of the tapered optical fiber with Au-NPs under the influence of hCG hormone concentrations, ranging from 1 mIU/mL to 100,000 mIU/mL. In the waist of the tapered optical fiber, the evanescent field is amplified because of localized surface plasmons generated by the nanoparticles and the laser radiation through the optical fiber. Experimental results demonstrated a proportional relationship between measured radiation power and hCG concentration, with the optical power response decreasing from 4.45 mW down to 2.5 mW, as the hCG hormone concentration increased from 1 mIU/mL up to 100,000 mIU/mL. Furthermore, the spectral analysis demonstrated a spectral shift of 14.2 nm with the increment of the hCG hormone concentration. The measurement system exhibits promising potential as a sensor for applications in the biomedical and industrial fields.

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“…Thanks to this technique, it is possible to detect the hormone at a very low level (1 mlU/ml), which will contribute to the possibility of detecting pregnancy at an early stage. [149] Another sensor described is a biosensor with field-effect transistors (FET) based on a single-layer MoS2 film and functionalized with Au NPs, which detects DNA fragments associated with Down syndrome. Escherichia coli JFS-Ag (Jack fruit seed extract) 4.75 μg/ml (MIC) 9.5 μg/ml (MBC)…”

“…The described optical fiber decorated with Au NPs is capable of detecting the hCG hormone; thanks to the NPs, the detection range and resolution have improved. Thanks to this technique, it is possible to detect the hormone at a very low level (1 mlU/ml), which will contribute to the possibility of detecting pregnancy at an early stage [149] . Another sensor described is a biosensor with field‐effect transistors (FET) based on a single‐layer MoS2 film and functionalized with Au NPs, which detects DNA fragments associated with Down syndrome.…”