Quantification of isoniazid in tablets for tuberculosis treatment by thin layer chromatography with smartphone image capture and ImageJ analysis

Sowers, Mary Elizabeth; Bethea, Ed; Xiao, Winnie; Phase, Nidhi; Harmon, Christopher; Jenkins, David

doi:10.1016/j.microc.2024.110796

Microchemical Journal

2024

DOI: 10.1016/j.microc.2024.110796

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Quantification of isoniazid in tablets for tuberculosis treatment by thin layer chromatography with smartphone image capture and ImageJ analysis

Mary Elizabeth Sowers,

Ed Bethea,

Winnie Xiao

et al.

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2024

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Quantification of Metronidazole in Tablets: Combining Thin-Layer Chromatography in the GPHF-Minilab™ with Image Processing Using Open-Source ImageJ Software

Harmon,

Butts,

Sowers

et al. 2024

Analytica

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The GPHF-Minilab™ is a portable toolkit for performing qualitative methods such as thin-layer chromatography (TLC) on common pharmaceuticals. It is particularly useful in resource-limited locations where it is more challenging to monitor for substandard and falsified (SF) medicines. However, the GPHF-Minilab™ TLC methods are only semi-quantitative at best and thus have issues monitoring product quality effectively. We have improved on the GPHF-Minilab™ TLC method for metronidazole, a common antibiotic, by making it fully quantitative. Sample solutions were spotted on TLC plates alongside three metronidazole standards at different concentrations. After development, plates were imaged in a lightbox with two different smartphone cameras. Images were processed through the open-source program ImageJ and resulting pixel data from the standard spots were used to create a calibration curve, enabling quantitation of the sample. The USP Metronidazole Tablet high-performance liquid chromatography (HPLC) assay was used as the reference method. We validated this TLC method using 250 and 500 mg metronidazole tablets from different manufacturers and assessed linearity, range, accuracy, precision, intermediate precision, specificity, and robustness. These improvements should enhance the GPHF-Minilab™ TLC methods for metronidazole product screening. Additionally, the procedure is extensible to other analytes, although further validation would be required for each Minilab method.

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Quantification of Metronidazole in Tablets: Combining Thin-Layer Chromatography in the GPHF-Minilab™ with Image Processing Using Open-Source ImageJ Software

Harmon,

Butts,

Sowers

et al. 2024

Analytica

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Quantification of isoniazid in tablets for tuberculosis treatment by thin layer chromatography with smartphone image capture and ImageJ analysis

Cited by 1 publication

References 21 publications

Quantification of Metronidazole in Tablets: Combining Thin-Layer Chromatography in the GPHF-Minilab™ with Image Processing Using Open-Source ImageJ Software

Quantification of Metronidazole in Tablets: Combining Thin-Layer Chromatography in the GPHF-Minilab™ with Image Processing Using Open-Source ImageJ Software

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