Quantification of melamine in human urine using cation-exchange based high performance liquid chromatography tandem mass spectrometry

Panuwet, Parinya; Nguyen, Johnny V.; Wade, Erin L; DʼSouza, Priya; Ryan, P. Barry; Barr, Dana Boyd

doi:10.1016/j.jchromb.2012.01.007

Cited by 61 publications

(38 citation statements)

References 20 publications

(9 reference statements)

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“…For the nonexposed workers, their urinary melamine concentrations, mostly ,2 mg/mmol, were similar to the general Caucasian and Asian populations. 7,8 However, urinary melamine concentrations in melamine workers in administrative areas were slightly higher than those in the general population, probably due to the adjacency of the workplace of the manufacturing process and consequent melamine particular "drift. "…”

Section: Discussionmentioning

confidence: 96%

“…7,8 One of the main sources is from the universal use of melamine tableware and kitchenware, 9,10 which are produced by melamine workers. Although the melamine workers in this study did not have abnormal clinical data of renal function, including serum BUN, creatinine, uric acid, eGFR and estimated creatinine clearance rate, and prominent abnormal renal morphology or increased renal stones by renal echo, preclinical renal injury markers have changed, as has been demonstrated in this study.…”

Section: Discussionmentioning

confidence: 99%

“…6 In 2012, Panuwet et al reported that 76% of 492 urine samples from the general United States population were detectable for melamine (.0.66 ng/ml of method of detection limit [MDL]). 7 Our recent data of 87 urine samples from 22 study children and 70 urine samples from their parents in one Taiwanese community revealed that in only two urine samples could melamine not be detected at a MDL of 0.8 ng/mL. 8 The aforementioned results indicated that both of these different ethnic populations are continuously exposed to melamine from the general environment.…”

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confidence: 96%

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Ambient Melamine Exposure and Urinary Biomarkers of Early Renal Injury

Peng

Liu

et al. 2015

Journal of the American Society of Nephrology

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Information about environmental exposure to melamine and renal injury in adults is lacking. We investigated this relationship in 44 workers at two melamine tableware manufacturing factories in Taiwan (16 manufacturers, eight grinders, ten packers, and ten administrators) and 105 nonexposed workers (controls) at one shipbuilding company who were enrolled in August-December of 2012. For melamine workers, personal and area air samples were obtained at the worksite over 1 workweek (Monday-Friday). In the same week, pre-and post-shift one-spot urine samples were collected each workday and one first-spot urine sample was collected on each weekend morning and the following Monday morning. For each control, a one-spot urine sample was collected on Friday morning. A blood sample was also obtained from each participant at this time. Melamine levels were measured in air, urine, and serum, and early renal injury biomarkers were measured in urine. Urinary melamine concentrations in manufacturers increased sharply between pre-and post-shift measurements on Monday, remained significantly elevated throughout the workweek, and decreased over the weekend; changes in urinary melamine concentrations were substantially lower for other melamine workers. Manufacturers were exposed to the highest concentrations of ambient melamine and had significantly higher urinary and serum melamine concentrations than did the controls (P,0.001). Urinary melamine levels were positively associated with urinary N-acetyl b-D-glucosaminidase (NAG) levels but not microalbumin levels, and the detectable b2-microglobulin rate increased in the manufacturers group. In conclusion, ambient melamine exposure may increase the levels of urinary biomarkers of renal tubular injury in this occupational setting.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 96%

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Ambient Melamine Exposure and Urinary Biomarkers of Early Renal Injury

Peng

Liu

et al. 2015

Journal of the American Society of Nephrology

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“…3 Excess MEL intake has led to serious health problems. [4][5][6][7][8][9] It has been reported that poisonous pet food resulting from deliberate MEL contamination severely impacted the health of thousands of dogs and cats between 2004 and 2007 in the USA. 10 Poisonous milk powder released in 2008 affected over 294,000 children and infants, of whom approximately 54,000 were hospitalized and six died in the People's Republic of China.…”

Section: Introductionmentioning

confidence: 99%

Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed

Wang¹,

Zhi²,

Zhao³

et al. 2014

IJN

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Although high melamine (MEL) intake has been proven to cause serious health problems, MEL is sometimes illegally added to milk products and animal feed, arousing serious food safety concerns. A satisfactory method of detecting MEL in onsite or in-home testing is in urgent need of development. This work aimed to explore a rapid, convenient, and cost-effective method of identifying MEL in milk products or other food by colloidal selenium-based lateral flow immunoassay. Colloidal selenium was synthesized by L-ascorbic acid to reduce seleninic acid at room temperature. After conjugation with a monoclonal antibody anti-MEL, a test strip was successfully prepared. The detection limit of the test strip reached 150 μg/kg, 1,000 μg/kg, and 800 μg/kg in liquid milk, milk powder, and animal feed, respectively. No cross-reactions with homologues cyanuric acid, cyanurodiamide, or ammelide were found. Moreover, the MEL test strip can remain stable after storage for 1 year at room temperature. Our results demonstrate that the colloidal selenium MEL test strip can detect MEL in adulterated milk products or animal feed conveniently, rapidly, and sensitively. In contrast with a colloidal gold MEL test strip, the colloidal selenium MEL test strip was easy to prepare and more cost-efficient.

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“…Sabe-se ainda da literatura, que algumas resinas de melamina são extratoras de metais através da interação iônica entre esta e o cloro-complexos desses íons [156][157][158] Melamina pode ser detectada na urina como um marcador de intoxicação [159,160], porém devido aos constantes relatos de adulteração alimentar, mais especificamente em leite, aplicou-se para a análise deste composto nessa matriz, porém, avaliou-se antes a possibilidade de interferência de algumas substancias presentes na matriz do leite. A Tabela 6 ilustra os resultados obtidos.…”

Section: Resultsunclassified

Desenvolvimento de sensores eletroquímicos e colorimétricos para aplicações em amostras de interesse forense

Araújo¹

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Quantification of melamine in human urine using cation-exchange based high performance liquid chromatography tandem mass spectrometry

Cited by 61 publications

References 20 publications

Ambient Melamine Exposure and Urinary Biomarkers of Early Renal Injury

Ambient Melamine Exposure and Urinary Biomarkers of Early Renal Injury

Lateral flow test strip based on colloidal selenium immunoassay for rapid detection of melamine in milk, milk powder, and animal feed

Desenvolvimento de sensores eletroquímicos e colorimétricos para aplicações em amostras de interesse forense

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