Quantification of Oligonucleotides Using Tandem Mass Spectrometry with Isobaric Internal Standards
Christopher Gawlig,
Güngör Hanci,
Michael Rühl
Abstract:In recent years, oligonucleotides have become more important in research, drug approvals and medical therapies. Due to this growing interest in pharmaceutical applications, it is essential to develop reliable analytical methods for this substance class. In this work, we present a quantification method using liquid chromatography coupled with tandem mass spectrometry by applying an isobaric oligonucleotide standard. In addition to a proof of principle, we perform a method qualification to assess its readiness f… Show more
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