Quantification of polyphenols with potential antioxidant properties in wines using reverse phase HPLC

Abstract

A RP-HPLC method with photodiode array detection (DAD) was developed to separate, identify and quantify simultaneously the most representative phenolic compounds present in Madeira and Canary Islands wines. The optimized chromatographic method was carefully validated in terms of linearity, precision, accuracy and sensitivity. A high repeatability and a good stability of phenolics retention times (a 3%) were obtained, as well as relative peak area. Also high recoveries were achieved, over 80.3%. Polyphenols calibration curves showed a good linearity (r 2 A0.994) within test ranges. Detection limits ranged between 0.03 and 11.5 lg/mL for the different polyphenols. A good repeatability was obtained, with intra-day variations less than 7.9%. The described method was successfully applied to quantify several polyphenols in 26 samples of different kinds of wine (red, rosØ and white wines) from Madeira and Canary Islands. Gallic acid was by far the most predominant acid. It represents more than 65% of all phenolics, followed by p-coumaric and caffeic acids. The major flavonoid found in Madeira wines was trans-resveratrol. In some wines, ( -)-epicatechin was also found in highest amount. Canary wines were shown to be rich in gallic, caffeic and p-coumaric acids and quercetin.

New method for determination of (E)‐resveratrol in wine based on microextraction using packed sorbent and ultra‐performance liquid chromatography

“…As it can be seen in Table 1, the MEPS C8 /ULPC-PDA methodology gave very low LODs (0.21 mg/mL) and LOQs (0.68 mg/mL). These limits are comparable with those obtained by other authors [44][45][46]48]. Table 1) and (B) influence of MEPS sorbents on (E)-resveratrol extraction efficiency (error bars represent standard error of the mean (n 5 3) for each data point).…”

“…Quantitative analysis of (E)-resveratrol is commonly performed by means of high-performance liquid chromatography (HPLC) connected to a diode array detector or a triple quadrupole mass spectrometer [26,27,[41][42][43][44][45][46][47][48]. In order to increase the selectivity and sensibility of the analytical method the analysis of these kinds of compounds should be performed by using a fluorescence detector.…”

A fast method using a new hydrophilic–lipophilic balanced sorbent in combination with ultra-high performance liquid chromatography for quantification of significant bioactive metabolites in wines

“…Curiously it was identified in one of the white wines tested (VGB wine from Canary Island). Our data about trans-resveratrol are in agreement with the results from Baptista et al [26,35] that found amounts of this compound in the range of 0.63-5.21 mg L −1 in other Portuguese red wines, including one from Azores Island.…”

“…These two polyphenols have being extensively shown to have several health benefits, namely in the cardiovascular and neurodegenerative protection [32,33]. Gallic acid, also often reported in wine composition [34,35], was identified in all wine samples, while syringic and p-coumaric acid seems to be more specific to white wines. In the same way, m-coumaric acid was only identified in white wines, while rutin was only detected in red wines.…”

Potent Antioxidative Activity of Vineatrol®30 Grapevine-shoot Extract

“…If one takes into account that the concentration of Resv in many red wines ranges between 0.5 and 10 mg/ml [18][19][20][21] and that the bioavailability of free Resv in humans is extremely low (1-2% at the most), 22,23) the amount of Resv used in the different assays of the present study was within the concentration range of Resv taken up when drinking one glass of red wine (125 ml) per day. In the case of "-viniferin, such a calculation cannot be made, since data on its bioavailability in humans are not available.…”