Quantification of product-specific gene expression in biopellets of Aspergillus niger with real-time PCR

Jungebloud, Anke; Bohle, Kathrin; Göcke, Yvonne; Cordes, Christiana; Horn, Harald; Hempel, D. C.

doi:10.1016/j.enzmictec.2006.05.022

Cited by 7 publications

(2 citation statements)

References 22 publications

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“…Reaction efficiencies 4 100% may indicate pipetting error or coamplification of nonspecific products, such as primer dimers (Dorak, 2006; BioRad bulletin #5306). However, as the development of qPCR is ongoing, efficiencies 4 105% are still reported (Atoui et al, 2007;Goebes et al, 2007;Jungebloud et al, 2007;Selma et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Specific detection ofAspergillus carbonariusby SYBR^Â®Green and TaqMan^Â®quantitative PCR assays based on the multicopy ITS2 region of the rRNA gene

González-Salgado

Patiño²,

Gil-Serna

et al. 2009

FEMS Microbiology Letters

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Agroproducts contaminated by ochratoxin A (OTA) represent a risk for human and animal health and, therefore, maximum limits have been established by Food Safety Authorities. Reduction of OTA contamination may be accomplished by early detection of OTA-producing fungal species using rapid, specific and sensitive detection and quantification by PCR-based methods. Aspergillus carbonarius is one of the most important OTA-producing species, in particular in grapes and derivatives from Mediterranean regions. In this work, highly efficient quantitative PCR assays using SYBR Green I and TaqMan methods were developed for specific detection of A. carbonarius to be used in grapes. The primers and the TaqMan probe were based on the internal transcribed region 2 multicopy region (internal 2 sequence of the rRNA gene). The specificity and sensitivity of both assays were tested on genomic DNA mixtures of several A. carbonarius strains and other fungal species frequently present in grapes. Both methods were also compared using grapes inoculated with different spore concentrations of A. carbonarius, detecting up to 0.4 pg DNA g(-1) grape berries. The efficiency and sensitivity of both methods were comparable and only the lower cost of SYBR Green might favour its use in routine screenings.

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Section: Discussionmentioning

confidence: 99%

Specific detection ofAspergillus carbonariusby SYBR^Â®Green and TaqMan^Â®quantitative PCR assays based on the multicopy ITS2 region of the rRNA gene

González-Salgado

Patiño²,

Gil-Serna

et al. 2009

FEMS Microbiology Letters

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“…Although a large number of microorganisms are capable of degrading cellulose, only few of them produce significant quantities of cell free cellulase capable of completely hydrolyzing crystalline cellulose in vitro (Jungebloud et al, 2007). Species of Aspergillus are well known and efficient producers of plant cell wall degrading enzyme system (Oksanen et al, 2000;Coral et al, 2002;Onsori et al, 2005).…”

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confidence: 99%

Cellulases Production by Free and Immobilized Aspergillus flavus

2014

Egypt. J. Bot.

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HE PRODUCTION of cellulase from some local fungal isolates ……..was studied. The results showed that Aspergillus flavus produced the highest cellulase activity (3.017 U/ml) under static condition. It also showed that the best carbon source for cellulase production was filter papers (7.0932 U/ml), and onion scales (6.396 U/ml) at a concentration 30 g/l medium. The Plackett-Burman design was studied for cellulase production from A. flavus showed that the optimizing medium achieved by the statistical design increased cellulase activity (11.699 U/ml) which approximately 2.3 times than that obtained from the basal medium. In addition, A. flavus biomass was entrapped in different gel materials, and the best one for producing cellulase was agar (8.22 U/ml), however, this activity was lower than that in free cultures. Moreover, A. flavus biomass was adsorbed on different porous materials, and the cultures adsorbed on luffa pulp exerted the highest cellulase activity (17.78 U/ml) which was higher than that produced by free cultures. By reusing the adsorbed A. flavus biomass on luffa pulp in seven successive times, the 4 th cycle gave the highest cellulase activity (34.17 U/ml). It was concluded that A. flavus is a feasible candidate for the production of cellulase activity which can be applied in many industrial fields. The results also indicate the possibility of using different agro-wastes like onion scales as a sole carbon source for production of cellulase by A. flavus.

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Evaluation of reference genes for quantitative real-time PCR normalization in the Neopyropia (Pyropia) oomycete pathogen Pythium porphyrae

et al. 2022

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Quantification of product-specific gene expression in biopellets of Aspergillus niger with real-time PCR

Cited by 7 publications

References 22 publications

Specific detection ofAspergillus carbonariusby SYBR^Â®Green and TaqMan^Â®quantitative PCR assays based on the multicopy ITS2 region of the rRNA gene

Specific detection ofAspergillus carbonariusby SYBR^Â®Green and TaqMan^Â®quantitative PCR assays based on the multicopy ITS2 region of the rRNA gene

Cellulases Production by Free and Immobilized Aspergillus flavus

Evaluation of reference genes for quantitative real-time PCR normalization in the Neopyropia (Pyropia) oomycete pathogen Pythium porphyrae

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Quantification of product-specific gene expression in biopellets of Aspergillus niger with real-time PCR

Cited by 7 publications

References 22 publications

Specific detection ofAspergillus carbonariusby SYBRÂ®Green and TaqManÂ®quantitative PCR assays based on the multicopy ITS2 region of the rRNA gene

Specific detection ofAspergillus carbonariusby SYBRÂ®Green and TaqManÂ®quantitative PCR assays based on the multicopy ITS2 region of the rRNA gene

Cellulases Production by Free and Immobilized Aspergillus flavus

Evaluation of reference genes for quantitative real-time PCR normalization in the Neopyropia (Pyropia) oomycete pathogen Pythium porphyrae

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Specific detection ofAspergillus carbonariusby SYBR^Â®Green and TaqMan^Â®quantitative PCR assays based on the multicopy ITS2 region of the rRNA gene

Specific detection ofAspergillus carbonariusby SYBR^Â®Green and TaqMan^Â®quantitative PCR assays based on the multicopy ITS2 region of the rRNA gene