Quantification of Some Phenolic Compounds in Consolida thirkeana (Boiss.) Bornm. by HPLC and Validation of Method

Abstract

Consolida species have traditional uses in the treatment of various diseases, especially skin diseases. There is also traditional use of some Consolida species in Turkey. Phenolic compounds have significant pharmacological effects and therefore it is important to know their amount in plants. Consolida thirkeana is endemic to Turkey and known as “boz mahmuz” and no study was found in terms of phenolic compounds. Therefore, some phenolic amount, which has been done for the first time for C. thikeana, was analysed. In this study, C. thirkeana was analysed quantitatively for caffeic acid, chlorogenic acid, hyperosit, and rutin by using HPLC and the method was validated (linearity, precision, accuracy, recovery, limits of detection (LOD), and limits of quantification (LOQ)). While chlorogenic acid (0.098%), caffeic acid (0.107%), rutin (0.078%), and hyperoside (0.134%) were detected in the aerial part, only rutin (0.007%) was detected in the root. As a result of this study, this endemic species was evaluated in terms of some phenolic compounds. It is thought that phenolic compounds can be determined on other Consolida species with this method.