Quantification of three antineoplastic agents in urine using the UniSpray ionisation source

Verscheure, Eline; Creta, Matteo; Vanneste, Dorian; Vanoirbeek, Jeroen; Meziane, Zakia; Taleb, A.; Lebegge, Robin; Poels, Katrien; Ghosh, Manosij; Duca, Radu Corneliu; Godderis, Lode

doi:10.1016/j.jchromb.2022.123331

Cited by 3 publications

(6 citation statements)

References 35 publications

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“…In the specific case of CP, Maekawa and co-workers presented some putative structures for the species formed under collision-induced dissociation (CID) from protonated CP based on the isotopic patterns of the chloride atoms and isotopically marked CP. High-resolution MS (HRMS) results from Verscheure and co-workers that also evaluated cyclophosphamide-d4 have confirmed these results …”

Section: Introductionsupporting

confidence: 81%

“…Korany and co-workers, in 2022, investigated the protonated CP fragmentation by ESI–MS and quantum mechanics . In this work, they reported fragments observed earlier in the literature and presented theoretical calculations to reason the structures proposed, confirming previous propositions. − , …”

Section: Introductionsupporting

confidence: 81%

“…49,50 The fragmentation to m/z 140 is the main transition used to qualitative and quantitative analyses of CP, and the signal intensity of this ion was comparable to the intensity of the ion with m/z 233. 25,27 To further evaluate the nature of the fragments, the ion with m/z 233, produced by loss of 28 Da (C 2 H 4 ), was evaluated by IRMPD spectroscopy, as shown in Figure 3a.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…High-resolution MS (HRMS) results from Verscheure and co-workers that also evaluated cyclophosphamide-d4 have confirmed these results. 25 Korany and co-workers, in 2022, investigated the protonated CP fragmentation by ESI−MS and quantum mechanics. 26 In this work, they reported fragments observed earlier in the literature and presented theoretical calculations to reason the structures proposed, confirming previous propositions.…”

Section: ■ Introductionmentioning

confidence: 99%

“…26 In this work, they reported fragments observed earlier in the literature and presented theoretical calculations to reason the structures proposed, confirming previous propositions. [23][24][25]27 To the best of the authors' knowledge, there have been no comprehensive experimental investigations conducted to verify the nature of the fragments' structures, notwithstanding these prior studies on the protonated and sodiated CP fragmentation pattern.…”

Section: ■ Introductionmentioning

confidence: 99%

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Protonated and Sodiated Cyclophosphamide Fragmentation Pathways Evaluation by Infrared Multiple Photon Dissociation Spectroscopy

Fernandes,

Obeid,

Laureno

et al. 2023

J. Phys. Chem. A

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Cyclophosphamide (CP or CTX) is a widely used antineoplastic agent, and the evaluation of its efficacy and its impacts on the environment are dependent on tandem mass spectrometry (MS n ) techniques. Because there is no dedicated experimental study to characterize the actual molecular nature of the CP fragments upon collision-induced dissociation, this work evaluated the chemical structure of the fragments of protonated and sodiated CP and CP protonation sites by infrared multiple photon dissociation spectroscopy supported by density functional theory calculations. This study allowed us to propose a new fragment structure and confirm the nature of multiple fragments, including those relevant for transitions used for CP quantitative and qualitative analyses. Our results also show that there is no spectroscopic evidence that can rule out the existence of aziridinium fragments, making it clear that further studies on the nature of iminium/aziridinium fragments in the gas phase are necessary.

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Section: Introductionsupporting

confidence: 81%

Section: Introductionsupporting

confidence: 81%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Protonated and Sodiated Cyclophosphamide Fragmentation Pathways Evaluation by Infrared Multiple Photon Dissociation Spectroscopy

Fernandes,

Obeid,

Laureno

et al. 2023

J. Phys. Chem. A

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Recent advancements in the chemical sensing of anticancer alkylating agents

Shanaah,

Allangawi,

ALZaimoor

et al. 2024

Chem. Pap.

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An Assessment of Surface Contamination and Dermal Exposure to 5-Fluorouracil in Healthcare Settings by UPLC-MS/MS Using a New Atmospheric Pressure Ionization Source

Creta,

Verscheure,

Tans

et al. 2024

Toxics

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5-Fluorouracil (5-FU) is a well-known cytostatic drug, which is often used in cancer treatments. Yet, it is also a very dangerous compound for people who are occupationally exposed to it for a long time, such as pharmacy employees, nurses and cleaning staff. We aimed to improve and implement a LC-MS/MS method for 5-FU quantification on surface contamination samples collected with swabs in a pharmacy department and outpatient nursing station of a university hospital. To improve the existing methods to quantify 5-FU, we compared a LC-MS/MS method using the frequently applied electrospray ionization source (ESI) with a UniSpray ionization source (USI). To determine the contamination of 5-FU in a pharmacy department preparing 5-FU infusion bags, which are then given to patients in the outpatient nursing stations, we collected multiple surface swabs of the laminar flow cabinets and frequently touched objects, before the preparation and administration of 5-FU and afterwards. Furthermore, we sampled the protective gloves and the bare hands of employees of the pharmacy department, involved in the preparation of the infusion bags. Using the USI source, we were able to reach the lowest limit of quantification (LOQ). With this technique, we were able to detect 5-FU contamination on the laminar flow cabinets and frequently used objects in the pharmacy department and the outpatient nursing station in the very low ng/cm2 range. This contamination was mostly higher after preparation or administration than before. While we also found 5-FU on the protective gloves, we almost found no 5-FU on the skin of the pharmacy technicians preparing the 5-FU infusion bags. In conclusion, our method was able to detect very low concentrations of 5-FU contamination, but the contamination we found is very unlikely to result in any issues for the personnel working in these areas.

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Quantification of three antineoplastic agents in urine using the UniSpray ionisation source

Cited by 3 publications

References 35 publications

Protonated and Sodiated Cyclophosphamide Fragmentation Pathways Evaluation by Infrared Multiple Photon Dissociation Spectroscopy

Protonated and Sodiated Cyclophosphamide Fragmentation Pathways Evaluation by Infrared Multiple Photon Dissociation Spectroscopy

Recent advancements in the chemical sensing of anticancer alkylating agents

An Assessment of Surface Contamination and Dermal Exposure to 5-Fluorouracil in Healthcare Settings by UPLC-MS/MS Using a New Atmospheric Pressure Ionization Source

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