Quantification of Tinto River Sediment Microbial Communities: Importance of Sulfate-Reducing Bacteria and Their Role in Attenuating Acid Mine Drainage

Sánchez‐Andrea, Irene; Knittel, Katrin; Amann, Rudolf; Amils, Ricardo; García, José Luis Sanz

doi:10.1128/aem.00848-12

Cited by 70 publications

(50 citation statements)

References 47 publications

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“…Syntrophobacter and Desulfosporosinus, two genera containing many species involved in sulfate reduction (58)(59)(60)(61), showed high relative abundances in libraries from Dapo Creek. Both Syntrophobacter and Desulfosporosinus have regularly been detected in AMD waters (62)(63)(64)(65). It is noteworthy that Desulfosporosinus species are able to reduce various metals, such as Fe(III), Mn(IV), and U(VI), in acidic solutions (pH 4.2), indicating a potential role of Desulfosporosinus species for biological treatment of AMD (66).…”

Section: Spatial Variations In Physiochemical Datamentioning

confidence: 99%

Diversity of the Sediment Microbial Community in the Aha Watershed (Southwest China) in Response to Acid Mine Drainage Pollution Gradients

Sun

Xiao

Sun

et al. 2015

Appl Environ Microbiol

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e Located in southwest China, the Aha watershed is continually contaminated by acid mine drainage (AMD) produced from upstream abandoned coal mines. The watershed is fed by creeks with elevated concentrations of aqueous Fe (total Fe > 1 g/liter) and SO 4 2؊ (>6 g/liter). AMD contamination gradually decreases throughout downstream rivers and reservoirs, creating an AMD pollution gradient which has led to a suite of biogeochemical processes along the watershed. In this study, sediment samples were collected along the AMD pollution sites for geochemical and microbial community analyses. High-throughput sequencing found various bacteria associated with microbial Fe and S cycling within the watershed and AMD-impacted creek. A large proportion of Fe-and S-metabolizing bacteria were detected in this watershed. The dominant Fe-and S-metabolizing bacteria were identified as microorganisms belonging to the genera Metallibacterium, Aciditerrimonas, Halomonas, Shewanella, Ferrovum, Alicyclobacillus, and Syntrophobacter. Among them, Halomonas, Aciditerrimonas, Metallibacterium, and Shewanella have previously only rarely been detected in AMD-contaminated environments. In addition, the microbial community structures changed along the watershed with different magnitudes of AMD pollution. Moreover, the canonical correspondence analysis suggested that temperature, pH, total Fe, sulfate, and redox potentials (E h ) were significant factors that structured the microbial community compositions along the Aha watershed.A cid mine drainage (AMD) is one of the most environmentally threatening by-products of the mining industry. The chemical and/or microbial weathering of metal sulfide-rich rocks such as pyrite (FeS 2 ), sphalerite (ZnS), and galena (PbS) produces sulfuric acid, which leads to the formation of AMD. AMD typically has low pH and elevated concentrations of sulfate and metals, which severely impair water and soil quality (1, 2).Despite the extreme toxicity and acidity, the AMD-associated environments harbor numerous acidophilic microorganisms (3, 4). Recently, molecular techniques have been extensively applied to study AMD microbiology, and a variety of acidophilic and metal-tolerant microorganisms have been identified (5). Prokaryotic microorganisms that are metabolically active in acidic niche boundaries (pH Ͻ 3) were found to be phylogenetically diversified, affiliated within the phyla Proteobacteria, Firmicutes, Acitinobacteria, Nitrospirae, and Acidobacteria (5). Many of these acidophiles are capable of Fe and S cycling (e.g., oxidation and reduction of these elements in appropriate geochemical and microbial environments) and thus play an important role both in the formation of acid mine waters and in natural attenuation of AMD. However, our understanding of the roles of geochemical factors in shaping microbial community structure and the potential of microorganisms in natural attenuation of AMD and AMD-impacted watersheds is still limited.The AMD of the Aha watershed in southwest China provides an ideal opportunity to ...

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Section: Spatial Variations In Physiochemical Datamentioning

confidence: 99%

Diversity of the Sediment Microbial Community in the Aha Watershed (Southwest China) in Response to Acid Mine Drainage Pollution Gradients

Sun

Xiao

Sun

et al. 2015

Appl Environ Microbiol

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“…The third sample was a mixture of acidic sediments that were collected from the Tinto River Basin (Huelva) in September 2011 at two sampling sites: JL (37.691207∞N, 6.560587∞W) and SN (37.72173∞N, 6.557465∞W) dams. Detailed information of the sampling sites was presented recently (Sánchez-Andrea et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH

Santos

Liebensteiner

Gelder

et al. 2018

J. Gen. Appl. Microbiol.

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“…They can utilize a wide spectrum of energy sources, ranging from aromatic compounds to short-chained fatty acids. A characteristic feature of many Desulfosporosinus species, distinguishing them from their closest sulfate-reducing relatives of the genus Desulfotomaculum, is their ability to grow chemolithoautotrophically on hydrogen (3,14,15,19,(21)(22)(23). Members of the genus Desulfosporosinus are found in low-sulfate freshwater and soil environments but also in sulfate-rich heavymetal-contaminated environments, such as acid mine/rock drainage sites.…”

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Complete Genome Sequences of Desulfosporosinus orientis DSM765^T, Desulfosporosinus youngiae DSM17734^T, Desulfosporosinus meridiei DSM13257^T, and Desulfosporosinus acidiphilus DSM22704^T

et al. 2012

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g Desulfosporosinus species are sulfate-reducing bacteria belonging to the Firmicutes. Their genomes will give insights into the genetic repertoire and evolution of sulfate reducers typically thriving in terrestrial environments and able to degrade toluene (Desulfosporosinus youngiae), to reduce Fe(III) (Desulfosporosinus meridiei, Desulfosporosinus orientis), and to grow under acidic conditions (Desulfosporosinus acidiphilus).T he sequenced Desulfosporosinus type strains (2,3,14,20) represent four out of eight described species belonging to the genus Desulfosporosinus and cover its phylogenetic and physiological breadth. Besides their ability to reduce sulfate for energy conservation, some Desulfosporosinus species can also grow by using nitrate, Fe(III), or As(V) as terminal electron acceptors or by fermentative processes. They can utilize a wide spectrum of energy sources, ranging from aromatic compounds to short-chained fatty acids. A characteristic feature of many Desulfosporosinus species, distinguishing them from their closest sulfate-reducing relatives of the genus Desulfotomaculum, is their ability to grow chemolithoautotrophically on hydrogen (3,14,15,19,(21)(22)(23). Members of the genus Desulfosporosinus are found in low-sulfate freshwater and soil environments but also in sulfate-rich heavymetal-contaminated environments, such as acid mine/rock drainage sites. In addition, Desulfosporosinus species are often observed in low-pH habitats (1, 3, 5-7, 12, 13, 17, 18), with the sequenced Desulfosporosinus acidiphilus strain being the first validly described sulfate-reducing acidophile (3).Genomic DNA was isolated using the Jetflex genomic DNA purification kit (GENOMED, Löhne, Germany) and subjected to sequencing using a combination of 454 Titanium (16) and Illumina (4) technologies. Sequences were assembled with Newbler (version 2.3-PreRelease-6/30/2009) and Velvet (version 1.0.13) (24) for 454 and Illumina data, respectively. Consensus sequences were obtained using computationally shredded Illumina and 454 reads together with 454 paired-end data using parallel Phrap (version SPS-4.24; High Performance Software, LLC). Identification of sequencing errors and improvement of consensus quality were done with Polisher (A. Lapidus, unpublished data) using Illumina data. GapResolution (C. Han, unpublished data), Dupfinisher (11), or sequencing cloned bridging PCR fragments with subcloning were used to correct misassemblies. Gaps between contigs were closed by editing in Consed (8-10), by PCR, and by Bubble PCR (J.-F. Cheng, unpublished data) primer walks. Automated genome annotation was performed at the Oak Ridge National Laboratory and is available at http://genome.ornl.gov/.The circular chromosomes of Desulfosporosinus orientis, Desulfosporosinus youngiae, Desulfosporosinus meridiei, and D. acidiphilus have sizes of 5,863,081 bp, 5,660,978 bp, 4,873,567 bp, and 4,926,837 bp, respectively. The genome of D. acidiphilus additionally harbors two plasmids of 60,447 bp and 3,897 bp. The genomes, in the order listed...

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Quantification of Tinto River Sediment Microbial Communities: Importance of Sulfate-Reducing Bacteria and Their Role in Attenuating Acid Mine Drainage

Cited by 70 publications

References 47 publications

Diversity of the Sediment Microbial Community in the Aha Watershed (Southwest China) in Response to Acid Mine Drainage Pollution Gradients

Diversity of the Sediment Microbial Community in the Aha Watershed (Southwest China) in Response to Acid Mine Drainage Pollution Gradients

Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH

Complete Genome Sequences of Desulfosporosinus orientis DSM765^T, Desulfosporosinus youngiae DSM17734^T, Desulfosporosinus meridiei DSM13257^T, and Desulfosporosinus acidiphilus DSM22704^T

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Quantification of Tinto River Sediment Microbial Communities: Importance of Sulfate-Reducing Bacteria and Their Role in Attenuating Acid Mine Drainage

Cited by 70 publications

References 47 publications

Diversity of the Sediment Microbial Community in the Aha Watershed (Southwest China) in Response to Acid Mine Drainage Pollution Gradients

Diversity of the Sediment Microbial Community in the Aha Watershed (Southwest China) in Response to Acid Mine Drainage Pollution Gradients

Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH

Complete Genome Sequences of Desulfosporosinus orientis DSM765T, Desulfosporosinus youngiae DSM17734T, Desulfosporosinus meridiei DSM13257T, and Desulfosporosinus acidiphilus DSM22704T

Contact Info

Product

Resources

About

Complete Genome Sequences of Desulfosporosinus orientis DSM765^T, Desulfosporosinus youngiae DSM17734^T, Desulfosporosinus meridiei DSM13257^T, and Desulfosporosinus acidiphilus DSM22704^T