Quantifiler ® Trio Kit and forensic samples management: A matter of degradation

Vernarecci, Stefano; Ottaviani, Enrica; Agostino, Alessandro; Mei, Elisabetta; Calandro, Lisa M.; Montagna, Paola

doi:10.1016/j.fsigen.2014.12.005

Cited by 79 publications

(41 citation statements)

References 15 publications

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“…Similar observations have been reported in the literature [7,8,21]. Our quantification method (63bp target) likely overestimated the amount of amplifiable DNA in these degraded samples.…”

Section: Tissuesupporting

confidence: 90%

“…Our quantification method (63bp target) likely overestimated the amount of amplifiable DNA in these degraded samples. We would expect that using a system such as Quantifiler ® Trio (ThermoFisher Scientific) or PowerQuant ® (Promega) with longer targets (>200 bases) would better correlate the amount of DNA detected with downstream STR results [7,20].…”

Section: Tissuementioning

confidence: 99%

“…Oxidative and hydrolytic damage may cause base modifications, strand breaks, crosslinks, and mismatches [4,5]. Prolonged exposure to heat, humidity, ultraviolet (UV) radiation, and microorganisms further increases the rate of DNA damage and degradation resulting in fragmentation of the DNA molecule into smaller lengths [4,6,7]. Although DNA typing using short tandem repeats (STRs) is currently the gold standard for human identification purposes [3], highly degraded samples often result in partial STR profiles because the larger loci (>250bp) commonly fail to amplify due to fragmentation of the DNA structure [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Preservation and rapid purification of DNA from decomposing human tissue samples

Sorensen

Rahman

Canela

et al. 2016

Forensic Science International: Genetics

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“…Similar observations have been reported in the literature [7,8,21]. Our quantification method (63bp target) likely overestimated the amount of amplifiable DNA in these degraded samples.…”

Section: Tissuesupporting

confidence: 90%

Section: Tissuementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preservation and rapid purification of DNA from decomposing human tissue samples

Sorensen

Rahman

Canela

et al. 2016

Forensic Science International: Genetics

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“…The degree of DNA degradation or fragmentation can be displayed as a degradation index (DI), where DNA quantity of the short target (80 bp) is divided by DNA quantity of the long target (207 or 214 bp) . Although DI can be accurately determined using a commercial DNA quantitation kit, the ratio of the mean peak height of the short amplicons to the mean peak height of the long amplicons can be utilized to roughly determine DI .…”

Section: Resultsmentioning

confidence: 99%

Differential pre‐amplification of STR loci for fragmented forensic DNA profiling

et al. 2016

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DNA profiling of short tandem repeats (STR) has been successfully used for the identification of individuals in forensic samples, accidents and natural disasters. However, STR profiling of DNA isolated from old crime scenes and damaged biological samples is difficult due to DNA degradation and fragmentation. Here, we show that pre-amplification of STR loci using biotinylated primers for the STR loci is an efficient strategy to obtain STR profiling results from fragmented forensic samples. Analysis of STR loci with longer amplicon sizes is generally hampered, since these relatively long loci are vulnerable to DNA fragmentation. This problem was overcome by using reduced or increased primer concentrations for loci with shorter or longer amplicon sizes, respectively, in our pre-amplification strategy. In addition, pre-amplification of STR loci into two groups of short or long amplicon size increases the efficiency of STR profiling from highly fragmented forensic DNA samples. Therefore, differential pre-amplification of STR loci is an effective way to obtain DNA profiling results from fragmented forensic samples.

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“…The workflow combining the DNA quantification with Quantifiler® Trio DNA quantification kit and STR amplification has the potencial to improve forensic laboratories efficiency [5].…”

Section: Resultsmentioning

confidence: 99%

Quantifiler ® Trio DNA validation and usefulness in casework samples

Vieira-Silva

Costa

Ribeiro

et al. 2015

Forensic Science International: Genetics Supplement Series

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DNA profiling with sets of highly polymorphic STR markers is applied in various aspects of human identification. During the past few years significant progress has been made in solving technical challenges associated with STR profiling, including the ability to analyze degraded DNA, low amounts of DNA and/or the presence of PCR inhibitors. Quantifiler® Trio DNA method enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human and human male DNA. Moreover, this kit has a set of primers to amplify and detected two autosomal multiple-copy target loci, known as the Small Autosomal (SA) and Large Autosomal (LA), which provide a quality index (DI) to detect the presence of degraded DNA along with PCR inhibitors. The experiments focus on kit performance parameters relevant to the intended use of the kits as DNA quantification assays and also application in casework studies. The workflow combining the DNA quantification with Quantifiler® Trio DNA quantification kit and STR amplification has the potencial to improve forensic laboratories efficiency.

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Quantifiler ® Trio Kit and forensic samples management: A matter of degradation

Cited by 79 publications

References 15 publications

Preservation and rapid purification of DNA from decomposing human tissue samples

Preservation and rapid purification of DNA from decomposing human tissue samples

Differential pre‐amplification of STR loci for fragmented forensic DNA profiling

Quantifiler ® Trio DNA validation and usefulness in casework samples

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