2023
DOI: 10.1021/acs.jpcb.3c04802
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Quantifying Bacteriorhodopsin Activity as a Function of its Local Environment with a Raman-Based Assay

Ryan E. Leighton,
Renee R. Frontiera

Abstract: Bacteriorhodopsin (bR) is a transmembrane protein that functions as a light-driven proton pump in halophilic archaea. The bR photocycle has been well-characterized; however, these measurements almost exclusively measured purified bR, outside of its native membrane. To investigate what effect the cellular environment has on the bR photocycle, we have developed a Raman-based assay that can monitor the activity of the bR in a variety of conditions, including in its native membrane. The assay uses two continuous-w… Show more

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