Quantifying Emission of Nir-I and Nir-Ii Dyes via Fluorescence Quantum Yield

Abstract

Fluorescence Quantum Yield allows scientists to both quantify spectroscopic properties of dyes and compare to literature references. With the growing interest in NIR-emissive dyes for biological imaging, it is of great importance to reliably measure the fluorescent quantum yield of these novel dyes. Using a broadband excitation source and liquid nitrogen cooled InGaAs detector, steady state emission of four novel pentamethine indolizine cyanine dyes synthesized with N,N-dimethylaniline-based substituents on the indolizine periphery at varied substitutions sites is recorded.