Quantifying Guest Exchange in Supramolecular Systems

Biomolecules undergo motions on the micro‐to‐millisecond timescale to adopt low‐populated transient states that play important roles in folding, recognition, and catalysis. NMR techniques, such as Carr–Purcell–Meiboom–Gill (CPMG), chemical exchange saturation transfer (CEST), and R1ρ are the most commonly used methods for characterizing such transitions at atomic resolution under solution conditions. CPMG and CEST are most effective at characterizing motions on the millisecond timescale. While some implementations of the R1ρ experiment are more broadly sensitive to motions on the micro‐to‐millisecond timescale, they entail the use of selective irradiation schemes and inefficient 1D data acquisition methods. Herein, we show that high‐power radio‐frequency fields can be used in CEST experiments to extend the sensitivity to faster motions on the micro‐to‐millisecond timescale. Given the ease of implementing high‐power fields in CEST, this should make it easier to characterize micro‐to‐millisecond dynamics in biomolecules.

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Extending the Sensitivity of CEST NMR Spectroscopy to Micro‐to‐Millisecond Dynamics in Nucleic Acids Using High‐Power Radio‐Frequency Fields

Rangadurai

Shi

Al‐Hashimi

2020

Angewandte Chemie

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Ein Methodenrepertoire für die paramagnetische NMR‐Spektroskopie zur Charakterisierung von paramagnetischen/Spin‐Crossover‐ Komplexen und Metall‐organischen Käfigverbindungen

et al. 2020

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Hintergrundinformationen und die Identifikationsnummer (ORCID) eines Autors sind unter https://doi.org/10.1002/ange.202008439 zu finden. 2020 Die Autoren. Verçffentlicht von Wiley-VCH GmbH. Dieser Open Access Beitrag steht unter den Bedingungend er Creative Commons AttributionL icense, die jede Nutzung des Beitragesi n allen Medien gestattet, sofern der ursprüngliche Beitrag ordnungsgemäßzitiert wird.

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Single Fluorinated Agent for Multiplexed ¹⁹F‐MRI with Micromolar Detectability Based on Dynamic Exchange

et al. 2021

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The weak thermal polarization of nuclear spins limits the sensitivity of MRI, even for MR-sensitive nuclei as fluorine-19. Therefore,d espite being the source of inspiration for the development of background-free MRI for various applications,including for multiplexed imaging,the inability to map very low concentrations of targets using 19 F-MRI raises the need to further enhance this platformsc apabilities.H ere, we employt he principles of CEST-MRI in 19 F-MRI to obtain a900-fold signal amplification of abiocompatible fluorinated agent, which can be presented in a" multicolor" fashion. Capitalizing on the dynamic interactions in host-guest supramolecular assemblies in an approach termed GEST,w e demonstrate that an inhalable fluorinated anesthetic can be used as as ingle 19 F-probe for the concurrent detection of micromolar levels of two targets,w ith potential in vivo translatability.F urther extending GEST with new designs could expand the applicability of 19 F-MRI to the mapping of targets that have so-far remained non-detectable.

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Quantifying Guest Exchange in Supramolecular Systems

Abstract: Scheme 1. a) Chemical structures of the hosts and fluorinated guests used. b) Dynamic exchange process studied.

Cited by 8 publications

References 54 publications

Extending the Sensitivity of CEST NMR Spectroscopy to Micro‐to‐Millisecond Dynamics in Nucleic Acids Using High‐Power Radio‐Frequency Fields

Extending the Sensitivity of CEST NMR Spectroscopy to Micro‐to‐Millisecond Dynamics in Nucleic Acids Using High‐Power Radio‐Frequency Fields

Ein Methodenrepertoire für die paramagnetische NMR‐Spektroskopie zur Charakterisierung von paramagnetischen/Spin‐Crossover‐ Komplexen und Metall‐organischen Käfigverbindungen

Single Fluorinated Agent for Multiplexed ¹⁹F‐MRI with Micromolar Detectability Based on Dynamic Exchange

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Quantifying Guest Exchange in Supramolecular Systems

Abstract: Scheme 1. a) Chemical structures of the hosts and fluorinated guests used. b) Dynamic exchange process studied.

Cited by 8 publications

References 54 publications

Extending the Sensitivity of CEST NMR Spectroscopy to Micro‐to‐Millisecond Dynamics in Nucleic Acids Using High‐Power Radio‐Frequency Fields

Extending the Sensitivity of CEST NMR Spectroscopy to Micro‐to‐Millisecond Dynamics in Nucleic Acids Using High‐Power Radio‐Frequency Fields

Ein Methodenrepertoire für die paramagnetische NMR‐Spektroskopie zur Charakterisierung von paramagnetischen/Spin‐Crossover‐ Komplexen und Metall‐organischen Käfigverbindungen

Single Fluorinated Agent for Multiplexed 19F‐MRI with Micromolar Detectability Based on Dynamic Exchange

Contact Info

Product

Resources

About

Single Fluorinated Agent for Multiplexed ¹⁹F‐MRI with Micromolar Detectability Based on Dynamic Exchange