Quantifying Skeletal Muscle Mitochondrial Function<i>In Vivo</i>by<sup>31</sup>P Magnetic Resonance Spectroscopy

Kemp, Graham J.

doi:10.1002/9781119329725.ch29

Cited by 2 publications

(3 citation statements)

References 87 publications

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“…The study of skeletal muscle metabolism is powerful because ATP synthesis is driven by ATP demand and metabolic demand is difficult to quantify in other tissues in vivo. MOP capacity is routinely assayed by near-infrared spectroscopy (NIRS) by measuring hemoglobin and myoglobin desaturation [55,56,57,58] and by phosphorus magnetic resonance spectroscopy ( 31 PMRS) measuring of phosphate metabolites [1,29,59,60,61,62,63]. In the present review, 31 PMRS is the primary method discussed because of its quantitative advantage measuring metabolites inherent to mitochondrial respiration while NIRS quantification of oxygen dynamics is an indirect measure of mitochondrial function.…”

Section: Measurement Of Mitochondrial Functionmentioning

confidence: 99%

Quantification of Mitochondrial Oxidative Phosphorylation in Metabolic Disease: Application to Type 2 Diabetes

Lewis

Kasper

Bazil

et al. 2019

IJMS

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Type 2 diabetes (T2D) is a growing health concern with nearly 400 million affected worldwide as of 2014. T2D presents with hyperglycemia and insulin resistance resulting in increased risk for blindness, renal failure, nerve damage, and premature death. Skeletal muscle is a major site for insulin resistance and is responsible for up to 80% of glucose uptake during euglycemic hyperglycemic clamps. Glucose uptake in skeletal muscle is driven by mitochondrial oxidative phosphorylation and for this reason mitochondrial dysfunction has been implicated in T2D. In this review we integrate mitochondrial function with physiologic function to present a broader understanding of mitochondrial functional status in T2D utilizing studies from both human and rodent models. Quantification of mitochondrial function is explained both in vitro and in vivo highlighting the use of proper controls and the complications imposed by obesity and sedentary lifestyle. This review suggests that skeletal muscle mitochondria are not necessarily dysfunctional but limited oxygen supply to working muscle creates this misperception. Finally, we propose changes in experimental design to address this question unequivocally. If mitochondrial function is not impaired it suggests that therapeutic interventions and drug development must move away from the organelle and toward the cardiovascular system.

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Section: Measurement Of Mitochondrial Functionmentioning

confidence: 99%

Quantification of Mitochondrial Oxidative Phosphorylation in Metabolic Disease: Application to Type 2 Diabetes

Lewis

Kasper

Bazil

et al. 2019

IJMS

View full text Add to dashboard Cite

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“…Selecting the most appropriate diagnostic technique requires a good understanding of ACS’s pathophysiology [ 17 ]. For the early diagnosis and treatment of ACS, it is necessary to evaluate muscle bioenergetics and metabolism non-invasively [ 7 , 16 , 18 , 19 , 20 , 21 , 22 ]. Magnetic resonance spectroscopy (MRS) is a unique technique for assessing tissue metabolic properties through the quantification of essential metabolites such as inorganic phosphate (Pi), phosphocreatine (PCr), and adenosine triphosphate (ATP) [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…For the early diagnosis and treatment of ACS, it is necessary to evaluate muscle bioenergetics and metabolism non-invasively [ 7 , 16 , 18 , 19 , 20 , 21 , 22 ]. Magnetic resonance spectroscopy (MRS) is a unique technique for assessing tissue metabolic properties through the quantification of essential metabolites such as inorganic phosphate (Pi), phosphocreatine (PCr), and adenosine triphosphate (ATP) [ 22 , 23 ]. Phosphorus magnetic resonance spectroscopy ( 31 P-MRS) has been used in clinical practice to evaluate high-energy phosphate metabolism changes of muscular and peripheral arterial diseases in humans [ 21 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Utilizing Dynamic Phosphorous-31 Magnetic Resonance Spectroscopy for the Early Detection of Acute Compartment Syndrome: A Pilot Study on Rats

Ohta

Hata

et al. 2021

Diagnostics

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Introduction: Disasters, including terrorism and earthquakes, are significant threats to people and may lead to many people requiring rescue. The longer the rescue takes, the higher the chances of an individual contracting acute compartment syndrome (ACS). ACS is fatal if diagnosed too late, and early diagnosis and treatment are essential. Objective: To assess the ability of dynamic phosphorus magnetic resonance spectroscopy (31P-MRS) in the early detection of muscular damage in ACS. Materials and Methods: Six ACS model rats were used for serial 31P-MRS scanning (9.4 Tesla). Skeletal muscle metabolism, represented by the levels of phosphocreatine (PCr), inorganic phosphate (Pi), and adenosine triphosphate (ATP), was assessed. The PCr/(Pi + PCr) ratio, which decreases with ischemia, was compared with simultaneously sampled plasma creatine phosphokinase (CPK), a muscle damage marker. Results: The PCr/(Pi + PCr) ratio significantly decreased after inducing ischemia (from 0.86 ± 0.10 to 0.18 ± 0.06; p < 0.05), while CPK did not change significantly (from 89 ± 29.46 to 241.50 ± 113.28; p > 0.05). The intracellular and arterial pH index decreased over time, revealing significant differences at 120 min post-ischemia (from 7.09 ± 0.01 to 6.43 ± 0.13, and from 7.47 ± 0.03 to 7.39 ± 0.04, respectively). In the reperfusion state, the spectra and pH did not return to the original values. Conclusions: The dynamic 31P-MRS technique can rapidly detect changes in muscle bioenergetics. This technique is a promising non-invasive method for determining early muscular damage in ACS.

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Quantifying Skeletal Muscle Mitochondrial FunctionIn Vivoby³¹P Magnetic Resonance Spectroscopy

Cited by 2 publications

References 87 publications

Quantification of Mitochondrial Oxidative Phosphorylation in Metabolic Disease: Application to Type 2 Diabetes

Quantification of Mitochondrial Oxidative Phosphorylation in Metabolic Disease: Application to Type 2 Diabetes

Utilizing Dynamic Phosphorous-31 Magnetic Resonance Spectroscopy for the Early Detection of Acute Compartment Syndrome: A Pilot Study on Rats

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Quantifying Skeletal Muscle Mitochondrial FunctionIn Vivoby31P Magnetic Resonance Spectroscopy

Cited by 2 publications

References 87 publications

Quantification of Mitochondrial Oxidative Phosphorylation in Metabolic Disease: Application to Type 2 Diabetes

Quantification of Mitochondrial Oxidative Phosphorylation in Metabolic Disease: Application to Type 2 Diabetes

Utilizing Dynamic Phosphorous-31 Magnetic Resonance Spectroscopy for the Early Detection of Acute Compartment Syndrome: A Pilot Study on Rats

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Product

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Quantifying Skeletal Muscle Mitochondrial FunctionIn Vivoby³¹P Magnetic Resonance Spectroscopy