Quantifying the dissolution of nanomaterials at the nano-bio interface

Zhang, Junzhe; He, Xiao; Zhang, Peng; Ma, Yuhui; Ding, Yayun; Wang, Zhengyu; Zhang, Zhiyong

doi:10.1007/s11426-015-5401-2

Cited by 11 publications

(6 citation statements)

References 52 publications

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“…[21,80,92] Therefore, more studies are needed to understand the toxicologically relevant chemical/biological processes at the nano/bio interface. [93][94][95] More importantly, experimental and theoretical studies are both needed to fully understand the detailed catalytic mechanisms of nanoceria. Although there are plenty of studies domenstrating the protective effects of nanoceria, only a few of them could provide direct evidence for the proposed ROS-scavenging-related mechanism.…”

Section: Perspective: Next Generation Nanoceriabased Enzyme Mimeticsmentioning

confidence: 99%

Ceria Nanoparticles as Enzyme Mimetics

Wang

Zhang

et al. 2017

Chin. J. Chem.

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In the past decades, enzyme mimetics based on ceria nanoparticles (nanoceria) have been developed as potential substitutes for nature enzymes. The mixed valence states of cerium and the patterns of oxygen vacancies on crystal planes result in different enzyme mimetic activities. In this review we survey the bio-applications of nanoceria-based enzyme mimetics as well as the underlying mechanisms. Factors influencing the enzyme mimetic activities and future perspective of nanoceria-based enzyme mimetics are also addressed.

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Section: Perspective: Next Generation Nanoceriabased Enzyme Mimeticsmentioning

confidence: 99%

Ceria Nanoparticles as Enzyme Mimetics

Wang

Zhang

et al. 2017

Chin. J. Chem.

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“…Although a number of studies have discussed the transformation of nanoparticles during toxicity testing, the extent and nature of these transformations is relatively difficult to qualitatively assess let alone to quantify. In the case of metal oxides, one of the major transformations could be particle dissolution which has been demonstrated for Zn, Cu and Ag-containing nanoparticles (see reviews by Ivask et al (2012) and Zhang et al (2015)). One of the main methods that has been used to study the dissolution of nanoparticles during toxicological testing has been separation of nanoparticulates and dissolved ions by centrifugation or membrane filtration.…”

Section: Introductionmentioning

confidence: 99%

Complete transformation of ZnO and CuO nanoparticles in culture medium and lymphocyte cells during toxicity testing

et al. 2017

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Here, we present evidence on complete transformation of ZnO and CuO nanoparticles, which are among the most heavily studied metal oxide particles, during 24 h in vitro toxicological testing with human T-lymphocytes. Synchrotron radiation-based X-ray absorption near edge structure (XANES) spectroscopy results revealed that Zn speciation profiles of 30 nm and 80 nm ZnO nanoparticles, and ZnSO- exposed cells were almost identical with the prevailing species being Zn-cysteine. This suggests that ZnO nanoparticles are rapidly transformed during a standard in vitro toxicological assay, and are sequestered intracellularly, analogously to soluble Zn. Complete transformation of ZnO in the test conditions was further supported by almost identical Zn spectra in medium to which ZnO nanoparticles or ZnSO was added. Likewise, Cu XANES spectra for CuO and CuSO-exposed cells and cell culture media were similar. These results together with our observation on similar toxicological profiles of ZnO and soluble Zn, and CuO and soluble Cu, underline the importance of dissolution and subsequent transformation of ZnO and CuO nanoparticles during toxicological testing and provide evidence that the nano-specific effect of ZnO and CuO nanoparticles is negligible in this system. We strongly suggest to account for this aspect when interpreting the toxicological results of ZnO and CuO nanoparticles.

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“…Dissolution studies on particle systems are often carried out by inductively coupled plasma mass spectrometry (ICP-MS), inductively coupled plasma atomic emission spectroscopy (ICP-AES), and atomic absorption spectroscopy (AAS), which are a few of the standards for quantitative analysis. However, the quantification of nanoparticle (NP) dissolution presents difficulties including the physical separation of NPs from the dissolved ionic species, insoluble precipitant formation, and achieving an equilibrium phase along with time and cost factors. ,,, In this work, we attempt to overcome some of these challenges and present simple methods using UV/vis absorbance spectra and spectrofluorometric monitoring to measure real-time dissolution kinetics of nZnO supported by ICP-MS and microscopy techniques. By taking the dissolution results into consideration, studies on cell viability were performed to determine how common buffering systems affect dissolution kinetics that impacts the structural transformation and cytotoxicity of nZnO.…”

Section: Introductionmentioning

confidence: 99%

Rapid Dissolution of ZnO Nanoparticles Induced by Biological Buffers Significantly Impacts Cytotoxicity

Eixenberger

Anders

Hermann

et al. 2017

Chem. Res. Toxicol.

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Zinc oxide nanoparticles (nZnO) are one of the most highly produced nanomaterials and are used in numerous applications including cosmetics and sunscreens despite reports demonstrating their cytotoxicity. Dissolution is viewed as one of the main sources of nanoparticle (NP) toxicity, however dissolution studies can be time-intensive to perform and complicated by issues such as particle separation from solution. Our work attempts to overcome some of these challenges by utilizing new methods using UV/vis and fluorescence spectroscopy to quantitatively assess nZnO dissolution in various biologically relevant solutions. All biological buffers tested induce rapid dissolution of nZnO. These buffers, including HEPES, MOPS, and PIPES, are commonly used in cell culture media, cellular imaging solutions and to maintain physiological pH. Additional studies using X-ray diffraction, FT-IR, X-ray photoelectron spectroscopy, ICP-MS and TEM were performed to understand how the inclusion of these non-essential media components impacts the behavior of nZnO in RPMI media. From these assessments, we demonstrate that HEPES causes increased dissolution kinetics, boosts the conversion of nZnO into zinc phosphate/carbonate and, interestingly, alters the structural morphology of the complex precipitates formed with nZnO in cell culture conditions. Cell viability experiments demonstrated that the inclusion of these buffers significantly decreases the viability of Jurkat leukemic cells when challenged with nZnO. This work demonstrates that biologically relevant buffering systems dramatically impact the dynamics of nZnO including dissolution kinetics, morphology, complex precipitate formation, and toxicity profiles.

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Quantifying the dissolution of nanomaterials at the nano-bio interface

Cited by 11 publications

References 52 publications

Ceria Nanoparticles as Enzyme Mimetics

Ceria Nanoparticles as Enzyme Mimetics

Complete transformation of ZnO and CuO nanoparticles in culture medium and lymphocyte cells during toxicity testing

Rapid Dissolution of ZnO Nanoparticles Induced by Biological Buffers Significantly Impacts Cytotoxicity

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