Quantifying the influence of polymer coatings on the serum albumin corona formation around silver and gold nanoparticles

Treuel, Lennart; Malissek, Marcelina; Graß, Stefan; Diendorf, Jörg; Mahl, Dirk; Meyer‐Zaika, Wolfgang; Epple, Matthias

doi:10.1007/s11051-012-1102-3

Cited by 51 publications

(62 citation statements)

References 97 publications

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“…Of note, layers of serum albumin and fibrinogen have been observed before on FePt NPs with ‐PMA and ‐PEG‐satPEG coating . Polymeric materials have been used previously to modulate the physico‐chemical characteristics of NPs and determine their effect on various parameters, including hydrophobicity, zeta potential, drug‐release properties, and biological behavior . This adsorption of large proteins to the NPs may confer conformational changes to the corona and affect subsequent protein adsorption to the particle surface.…”

Section: Resultsmentioning

confidence: 99%

Colloidal Stability and Surface Chemistry Are Key Factors for the Composition of the Protein Corona of Inorganic Gold Nanoparticles

Johnston

Kreyling

Pfeiffer

et al. 2017

Adv Funct Materials

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To study the influence of colloidal stability on protein corona formation, gold nanoparticles are synthesized with five distinct surface modifications: coating with citric acid, bis(p-sulfonatophenyl)phenylphosphine dihydrate dipotassium salt, thiol-terminated methoxy-polyethylene glycol, dodecylaminegrafted poly(isobutylene-alt-maleic anhydride), and dodecylamine-grafted poly(isobutylene-alt-maleic anhydride) conjugated with polyethylene glycol. The nanoparticles are incubated with serum or bronchoalveolar lavage fluid from C57BL/6 mice (15 min or 24 h) to assess the effect of differential nanoparticle surface presentation on protein corona formation in the air-blood barrier exposure pathway. Proteomic quantification and nanoparticle size measurements are used to assess protein corona formation. We show that surface modification has a clear effect on the size and the composition of the protein corona that is related to the colloidal stability of the studied nanoparticles. Additionally, differences in the composition and size of the protein corona are shown between biological media and duration of exposure, indicating evolution of the corona through this exposure pathway. Consequently, a major determinant of protein corona formation is the colloidal stability of nanoparticles in biological media and chemical or environmental modification of the nanoparticles alters the surface presentation of the functional epitope in vivo. Therefore, the colloidal stability of nanoparticles has a decisive influence on nano-bio interactions.

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Section: Resultsmentioning

confidence: 99%

Colloidal Stability and Surface Chemistry Are Key Factors for the Composition of the Protein Corona of Inorganic Gold Nanoparticles

Johnston

Kreyling

Pfeiffer

et al. 2017

Adv Funct Materials

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“…A layer of proteins forms on the NP surfaces, the so-called protein corona [10,[31][32][33][34][35][36][37][38][39][40][41][42][43]. Consequently, cell surface receptors, which activate the endocytosis machinery, actually encounter NPs enshrouded in biomolecules rather than bare particles.…”

Section: Introductionmentioning

confidence: 99%

New views on cellular uptake and trafficking of manufactured nanoparticles

Treuel

Jiang

Nienhaus

2013

J. R. Soc. Interface.

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315

224

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Nanoparticles (NPs) are of similar size to typical cellular components and proteins, and can efficiently intrude living cells. A detailed understanding of the involved processes at the molecular level is important for developing NPs designed for selective uptake by specific cells, for example, for targeted drug delivery. In addition, this knowledge can greatly assist in the engineering of NPs that should not penetrate cells so as to avoid adverse health effects. In recent years, a wide variety of experiments have been performed to elucidate the mechanisms underlying cellular NP uptake. Here, we review some select recent studies, which are often based on fluorescence microscopy and sophisticated strategies for specific labelling of key cellular components. We address the role of the protein corona forming around NPs in biological environments, and describe recent work revealing active endocytosis mechanisms and pathways involved in their cellular uptake. Passive uptake is also discussed. The current state of knowledge is summarized, and we point to issues that still need to be addressed to further advance our understanding of cellular NP uptake.

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“…It also indicates that the hydrodynamic diameter of AuNP-HSA complexes increases with the increase of protein molecules per gold nanoparticle. 21 The number of bound proteins per NP is clearly an average value, as it is safe to assume that in solution there will be a distribution in the number of bound HSA molecules to each nanoparticle for the different initial NP-protein ratios. Similar values (3.5 nm) have been found in the case of HSA forming a protein corona around the FePt nanoparticles as measured by fluorescence correlation spectroscopy.…”

mentioning

confidence: 99%

Determination of the structure and morphology of gold nanoparticle–HSA protein complexes

et al. 2015

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We propose a simple method to determine the structure and morphology of nanoparticle protein complexes. By combining a separation method with online size measurements, density measurements and circular dichroism, we could identify the number of proteins bound to each nanoparticle and their secondary structure changes in the complex. This method provides much-needed experimental information on the interaction of proteins with nanoparticles and on the behavior of nanoparticles in biological systems.

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Quantifying the influence of polymer coatings on the serum albumin corona formation around silver and gold nanoparticles

Cited by 51 publications

References 97 publications

Colloidal Stability and Surface Chemistry Are Key Factors for the Composition of the Protein Corona of Inorganic Gold Nanoparticles

Colloidal Stability and Surface Chemistry Are Key Factors for the Composition of the Protein Corona of Inorganic Gold Nanoparticles

New views on cellular uptake and trafficking of manufactured nanoparticles

Determination of the structure and morphology of gold nanoparticle–HSA protein complexes

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