Quantifying the vascular response to ischemia with speckle variance optical coherence tomography

Abstract

Abstract:Longitudinal monitoring techniques for preclinical models of vascular remodeling are critical to the development of new therapies for pathological conditions such as ischemia and cancer. In models of skeletal muscle ischemia in particular, there is a lack of quantitative, non-invasive and long term assessment of vessel morphology. Here, we have applied speckle variance optical coherence tomography (OCT) methods to quantitatively assess vascular remodeling and growth in a mouse model of peripheral arterial disease. This approach was validated on two different mouse strains known to have disparate rates and abilities of recovering following induction of hind limb ischemia. These results establish the potential for speckle variance OCT as a tool for quantitative, preclinical screening of pro-and anti-angiogenic therapies. ©2014 Optical Society of America

Visualization of conventional outflow tissue responses to netarsudil in living mouse eyes

“…Also, the outcome of Schlemm II containing all vessels is still available. Based on these segmented regions, speckle variance regions generated by blood cells or other reflectors in vessels (Hendargo et al, 2013; Huang et al, 2009; Li et al, 2014a; Mariampillai et al, 2008; Poole et al, 2014) were automatically segmented by the third version of the software, Schlemm III, that includes several statistical analysis tests to measure the shape, size, location and variation of speckling in different vessels as well as the SC.…”

ROS-responsive microspheres for on demand antioxidant therapy in a model of diabetic peripheral arterial disease

“…At day 7 post-surgery, images of the hind limb vasculature were collected through the skin non-invasively using a swept-source optical coherence tomography (OCT) system with a 1060 nm, 100 kHz source (Axsun Technologies, Inc.) [50, 55]. Speckle variance OCT volumes [56] were collected in a 4 mm × 4 mm area covering the gastrocnemius muscle region to monitor remodeling of vessels in response to hind limb ischemia [39].…”

“…Speckle variance OCT volumes [56] were collected in a 4 mm × 4 mm area covering the gastrocnemius muscle region to monitor remodeling of vessels in response to hind limb ischemia [39]. The speckle variance B-scans were processed as described previously [50, 55], and an average intensity projection over ~1.5 mm in depth was computed to visualize all vessels within the volume in a 2D image. The projection images of the vasculature were filtered to enhance contrast and connectivity [50, 55, 57], and the distribution of vessel diameters in each image was quantified.…”

Copolymer‐Mediated Cell Aggregation Promotes a Proangiogenic Stem Cell Phenotype In Vitro and In Vivo

“…Speckle variance optical coherence tomography (OCT) was used for in vivo imaging [17] . To visualize projection images of the vasculature within the implanted scaffolds, volume data sets were processed with a speckle variance technique that computes the variance in each pixel over seven repeated A-lines at each lateral position.…”