Quantised transistor response to ion channels revealed by nonstationary noise analysis

Becker-Freyseng, Christoph; Fromherz, Peter

doi:10.1209/0295-5075/96/38005

Cited by 5 publications

(9 citation statements)

References 27 publications

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“…Because the HEK-293 cell were genetically modified so that the membrane contains only K + channels, [213] the opening and closing of these ion channels could be unambiguously identified by monitoring the current across the membrane with the patch electrodes. [68] Simultaneously, signals from the GFET could be attributed to these K + channels and very good SNR (up to fifty) could be achieved by repeating the same process multiple times.…”

Section: Gfet Biological Cellular Sensorsmentioning

confidence: 99%

Sensing at the Surface of Graphene Field‐Effect Transistors

et al. 2016

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Abstract. 10Recent research trends now offer new opportunities for developing the next generations of label-free biochemical sensors using graphene and other two-dimensional materials. While the physics of graphene transistors operated in electrolyte is well grounded, important chemical challenges still remain to be addressed, namely the impact of the chemical functionalizations of graphene on the key electrical parameters and the sensing performances. In fact, graphene -at least ideal graphene -is highly chemically inert. The 15 functionalizations and chemical alterations of the graphene surface -both covalently and non-covalently -are crucial steps that define the sensitivity of graphene. The presence, reactivity, adsorption of gas and ions, proteins, DNA, cells and tissues on graphene have been successfully monitored with graphene. This review aims to unify most of the work done so far on biochemical sensing at the surface of a (chemically functionalized) graphene field-effect transistor and the challenges that lie ahead. We are convinced that graphene biochemical sensors 20 hold great promises to meet the ever-increasing demand for sensitivity, especially looking at the recent progresses suggesting that the obstacle of Debye screening can be overcome.2

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Section: Gfet Biological Cellular Sensorsmentioning

confidence: 99%

Sensing at the Surface of Graphene Field‐Effect Transistors

et al. 2016

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“…[57] The cells used in our experiments are genetically modified in such a way, that their membrane contains only one type of ion channels, in our case potassium channels. [58] Therefore, all observed currents and transistor signals can be attributed to these channels which simplifies the analysis significantly. Figure 14 shows the results of such a patch-clamp experiment.…”

Section: Human Kidney Cells (Hek-293)mentioning

confidence: 99%

Graphene Transistors for Bioelectronics

2013

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“…By transfection with Shaker B Delta6–46 T449V, voltage‐gated potassium channels were introduced into the cell line. The culture was done in Dulbecco's modified Eagle's medium (DMEM, high glucose, Sigma) with 2 m m L‐glutamine, hygromycin B (200 μg/ml) and 10% heat‐inactivated fetal bovine serum (FBS, Invitrogen) as described previously …”

Section: Methodsmentioning

confidence: 99%

“…The pipette and transistor currents were fed to a data acquisition system (NI USB‐6259), which is connected to a computer. A more detailed description of the recording system has been reported elsewhere …”

Section: Methodsmentioning

confidence: 99%

“…The culture was done in Dulbecco's modifi ed Eagle's medium (DMEM, high glucose, Sigma) with 2 m M L-glutamine, hygromycin B (200 µg/ml) and 10% heat-inactivated fetal bovine serum (FBS, Invitrogen) as described previously. [ 26 ] For the transistor recordings, the chips were sterilized by immersion in 70% ethanol for 20 minutes. After rinsing with phosphate buffered saline (PBS), fi bronectin was adsorbed (12 µg ml −1 ) on the chips and incubated (2 h).…”

Section: Methodsmentioning

confidence: 99%

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Electrical Coupling Between Cells and Graphene Transistors

Hess

Becker-Freyseng

Wismer

et al. 2014

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In this work, both experimental data and a model are presented on the coupling between living cells and graphene solution-gated field-effect transistors. Modified HEK 293 cells are successfully cultured on graphene transistor arrays and electrically accessed by the patch clamp method. Transistor recordings are presented, showing the opening and closing of voltage-gated potassium ion channels in the cell membrane. The experimental data is compared with the broadly used standard point-contact model. The ion dynamics in the cell-transistor cleft are analyzed to account for the differences between the model and the experimental data revealing a significant increase in the total ionic strength in the cleft. In order to describe the influence of the ion concentration resulting from the cell activity, the ion-sensitivity of graphene solution-gated field-effect transistors is investigated experimentally and modelled by considering the screening effect of the ions on the surface potential at the graphene/electrolyte interface. Finally, the model of the cell-transistor coupling is extended to include the effect of ion accumulation and ion sensitivity. The experimental data shows a very good agreement with this extended model, emphasizing the importance of considering the ion concentration in the cleft to properly understand the cell-transistor coupling.

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Quantised transistor response to ion channels revealed by nonstationary noise analysis

Cited by 5 publications

References 27 publications

Sensing at the Surface of Graphene Field‐Effect Transistors

Sensing at the Surface of Graphene Field‐Effect Transistors

Graphene Transistors for Bioelectronics

Electrical Coupling Between Cells and Graphene Transistors

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