Quantitation of human IgG subclass antibodies to Haemophilus influenzae type b capsular polysaccharide

Herrmann, Diane J.; Hamilton, Robert G.; Barington, Torben; Frasch, Carl E.; Arakere, Gayathri; Mãkelä, O.; Mitchell, Leslie A.; Nagel, J.; Rijkers, Ger T.; Zegers, B. J. M.; Danve, B.; Ward, Joel I.; Brown, Caroline

doi:10.1016/0022-1759(92)90163-n

Cited by 31 publications

(19 citation statements)

References 19 publications

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“…Hib-specific sequences included mainly IgA 2 and IgG 2 sequences (Fig. S4B), which is consistent with previous work demonstrating that IgG 2 anti-Hib antibodies are the predominant IgG subclass in adult sera (57). Sequences that were identified as TT-specific (i.e.…”

Section: Discussionsupporting

confidence: 92%

Identification of Antigen-Specific B Cell Receptor Sequences Using Public Repertoire Analysis

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Ramasamy

Galson

et al. 2015

The Journal of Immunology

114

105

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High-throughput sequencing allows detailed study of the B cell receptor (BCR) repertoire post-immunization but it remains unclear to what extent the de novo identification of antigen-specific sequences from the total BCR repertoire is possible. A Hib-MenC-TT conjugate vaccine containing H. influenzae type b (Hib) and group C meningococcal (MenC) polysaccharides as well as tetanus toxoid (TT) was used to investigate the BCR repertoire of adult humans following immunization and test the hypothesis that public or convergent repertoire analysis could identify antigen specific sequences. A number of antigen-specific BCR sequences have previously been reported for Hib and TT which made a vaccine containing these 2 antigens an ideal immunological stimulus. Analysis of identical complementarity determining region (CDR)3 amino acid (AA) sequences that were shared by individuals in the post-vaccine repertoire identified a number of known Hib-specific sequences but only one previously described TT sequence. The extension of this analysis to non-identical but highly similar CDR3 AA sequences revealed a number of other TT-related sequences. The anti-Hib avidity index post-vaccination was strongly correlated with the relative frequency of Hib-specific sequences, indicating that the post-vaccination public BCR repertoire may be related to more conventional measures of immunogenicity correlating with disease protection. Analysis of public BCR repertoire provided evidence of convergent BCR evolution in individuals exposed to the same antigens. If this finding is confirmed, the public repertoire could be used for rapid and direct identification of protective antigen-specific BCR sequences from peripheral blood.

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Section: Discussionsupporting

confidence: 92%

Identification of Antigen-Specific B Cell Receptor Sequences Using Public Repertoire Analysis

Trück

Ramasamy

Galson

et al. 2015

The Journal of Immunology

114

105

View full text Add to dashboard Cite

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“…Our data are similar to the response seen in adults to IgG2 1 IgG1 other polysaccharide antigens such as Hib [31,32], pneumococcus [17,33,34], and group A streptococcus [35] but differ from other studies in which the response to MAPS [27] and Hib [36] showed predominant IgG1 subclass antibodies in some individuals with IgG2 predominating in others.…”

Section: Discussionsupporting

confidence: 92%

“…Previous studies on the human response to bacterial polysaccharides show that, of all the class and subclass Ig chains, IgG2-specific antibodies correlate best with serum bactericidal activity and protection [31,34], whereas IgG1 antibodies function better as opsonins and may be superior to IgG2 in protection against infection [41]. IgM antibodies have been shown to be bactericidal but function poorly as opsonins [40].…”

Section: Discussionmentioning

confidence: 99%

Elderly Immune Response to a TI‐2 Antigen: Heavy and Light Chain Use and Bactericidal Activity toNeisseria meningitidisSerogroup C Polysaccharide

1999

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Previous studies of the elderly immune response to TI-2 antigens failed to correlate specific antibody levels with function and to compare responses with those of young adults. Neisseria meningitidis serogroup C capsular polysaccharide (MCPS) was used as a model TI-2 antigen. Anti-MCPS antibody levels were determined in elderly individuals and correlated with bactericidal activity. The anti-MCPS response in most persons was characterized by predominant IgG usage, with IgG2>IgG1. No light chain or IgA subclass predominated, but some responses showed a particular chain type. Bactericidal activity correlated best with IgG2 levels. Elderly subjects had lower anti-MCPS responses than the young adults did in all chain-specific anti-MCPS levels, and levels declined more rapidly. Bactericidal activity following immunization was significantly lower in the elderly persons. These results suggest the anti-MCPS antibody repertoire in the elderly is likely maintained, and the lower level of function is related to the lower antibody levels.

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“…Generally, children respond to bacterial polysaccharides with more IgG1 antibodies and adults with more IgG2 antibodies (18,19). In children 18 mo of age, there is a high proportion of nonresponders with IgG2 antibodies (20,21).…”

Section: Discussionmentioning

confidence: 99%

Birth weight predicts response to vaccination in adults born in an urban slum in Lahore, Pakistan

Moore

Jalil

Ashraf

et al. 2004

The American Journal of Clinical Nutrition

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Background: Substantial evidence exists linking small size at birth to later-life susceptibility to chronic disease. Evidence is also emerging that some components of immune function may be programmed in early life. However, this evidence is limited and requires confirmation. Objective: We investigated the association between size at birth and response to vaccination in a cohort of 257 adults (mean age: 29.4 y; 146 men) born in an urban slum in Lahore, Pakistan, during 1964Pakistan, during -1978. Design: A single dose of Vi polysaccharide vaccine for Salmonella typhi and 2 doses of rabies vaccine were given to each subject. Antibody titers were measured in prevaccination serum samples (Vi) and in postvaccination samples (Vi and rabies). Results:The mean birth weight of the subjects was 3.24 kg; 14% of the subjects had low birth weights (2.5 kg). Vaccine responses were not consistently associated with contemporary variables (month of study, sex, current age, or indicators of wealth). Response to typhoid vaccination was positively related to birth weight (anti-Vi immunoglobulin G: r ҃ 0.138, P ҃ 0.031; anti-Vi immunoglobulin M: r ҃ 0.197, P ҃ 0.034). Response to the rabies vaccine was not significantly associated with birth weight. Conclusions: These findings add to a growing body of evidence suggesting that components of the immune system may be permanently programmed by events in early life. The contrasting effects on typhoid and rabies responses suggest that antibody generation to polysaccharide antigens, which have greater B cell involvement, is compromised by fetal growth retardation.Am J Clin Nutr 2004; 80:453-9.

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Quantitation of human IgG subclass antibodies to Haemophilus influenzae type b capsular polysaccharide

Cited by 31 publications

References 19 publications

Identification of Antigen-Specific B Cell Receptor Sequences Using Public Repertoire Analysis

Identification of Antigen-Specific B Cell Receptor Sequences Using Public Repertoire Analysis

Elderly Immune Response to a TI‐2 Antigen: Heavy and Light Chain Use and Bactericidal Activity toNeisseria meningitidisSerogroup C Polysaccharide

Birth weight predicts response to vaccination in adults born in an urban slum in Lahore, Pakistan

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