Quantitative analysis of carbohydrates using gas-liquid chromatography

Richey, Jane M.; Richey, Herman G.; Schraer, Rosemary

doi:10.1016/0003-2697(64)90185-x

Cited by 61 publications

(14 citation statements)

References 6 publications

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“…Nor is there a preferred order among the sugars. Rickey et al (4) used 50 ILl of HMDS and 30 ILl of TMS for solutions containing up to 10 mg of sugars in 0.1 ml of pyridine. Brobst and Lott (I) used 0.9 ml of HMDS and 0.1 ml TFA per 70 mg of sugar and got resolution of the sugars found in corn syrup.…”

Section: Resultsmentioning

confidence: 99%

“…Sweeley et al (6) also found excellent separation with configurational pairs as well as anomeric pairs within given classes. Rickey et al (4) described the quantitativ(: analysis of various monosaccharides, using the trinethylsilyl derivatives and GLC with a constant column temperature and an argon detector. They reported accuracy within 10% on sugar mixtures by using standardization graphs.…”

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confidence: 99%

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Adaptation of Gas‐Liquid Chromatography to Analysis of Certain Sugars of Cotton Leaves¹

Sabbe¹,

Cathey²

1970

Agronomy Journal

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A procedure involving gas‐liquid chromatography (GLC) of certain sugars in cotton leaves was studied. The use of hexamethyl‐disilazane (HMDS) and trimethyl‐chlorosilane (TMS) for silylation of fructose, glucose, and sucrose gave recovery percentages ranging from 71 to 105%. The substitution of trifluoracetic acid (TFA) for TMS gave recovery percentages of 91 to 109%. The use of TFA also improves the ease of sample preparation since absolute exclusion of water was not necessary. Details of sample preparation and operating parameters of GLC are given.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Adaptation of Gas‐Liquid Chromatography to Analysis of Certain Sugars of Cotton Leaves¹

Sabbe¹,

Cathey²

1970

Agronomy Journal

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“…Total hexoses were determined by the method of Walborg and Christensson (1965) and individual sugars were detected by gas-liquid chromatography of their trimethylsilyl derivatives on 3% OV-17 (Applied Science Laboratories, Inc ., State College, Pa .) (Richey et al ., 1964) . Amino sugars were analyzed by the Elson-Morgan reaction (Blix, 1948) .…”

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confidence: 99%

PLASMA AND PHAGOSOME MEMBRANES OF ACANTHAMOEBA CASTELLANII

Ulsamer

Wright

Wetzel

et al. 1971

The Journal of Cell Biology

116

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Plasma membranes were isolated from the ameba Acanthamoeba castellanii by low-speed velocity centrifugation followed by equilibrium centrifugation in a sucrose gradient . The isolated membranes had a high ratio of sterol to phospholipid (0 .98 moles/mole) and of phospholipid to protein (0.43 mg/mg) . The plasma membranes had very low concentrations of DNA, RNA, lipid inositol, and glycerides . Glycolipids and glycoproteins were enriched in the plasma membranes relative to their concentrations in the whole cell . The plasma membranes were also judged to be of high purity by the absence, or very low level, of enzymatic activities considered to be indicative of other cell membranes, and by electron microscope examination . Alkaline phosphatase and 5'-nucleotidase activities were enriched in the plasma membranes 13-fold relative to the whole homogenate and had higher specific activities in the plasma membranes than in any other cell fractions . A Mg++ adenosine triphosphatase (ATPase) was enriched sixfold in the plasma membranes relative to the whole homogenate . The phospholipids of the plasma membranes contained more phosphatidylethanolamine and phosphatidylserine and less phosphatidylcholine than did the phospholipids of the whole cells . There were differences in the fatty acid compositions of corresponding phospholipids in the plasma membranes and whole cells but no difference in the ratios of total saturated to unsaturated fatty acids . The membranes of phagosomes isolated from amebae that had ingested polystyrene latex had essentially the same phospholipid, sterol, and enzymatic composition as plasma membranes.

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“…The triniethylsilyl derivatives of sugars was also subject to investigation (36,404,476), as were also the same derivat,ives of vit,amins D2 and DB (355) and myoinositol (411).…”

Section: Gas Chromatographymentioning

confidence: 99%

Biochemical Analysis

Hamilton¹

1966

Anal. Chem.

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review covers the two year period commencing January 1, 1964 through December 31,1965, following directly on the previous review of the same title (201) in Analytical Chemistry.Those papers which describe advances and improvements in methods and instrumentation as applied to determinations in the field of biochemistry are listed.Routine applications of standard methods are not reported. For these reasons, references to many excellent papers were omitted. Even with such an arbitrary selection, many papers of importance and interest had to be noted and as a consequence critical appraisal had to be virtually eliminated.COMMUNICATION AIDS, NEW JOURNALS, AND NEW BOOKS

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Quantitative analysis of carbohydrates using gas-liquid chromatography

Cited by 61 publications

References 6 publications

Adaptation of Gas‐Liquid Chromatography to Analysis of Certain Sugars of Cotton Leaves¹

Adaptation of Gas‐Liquid Chromatography to Analysis of Certain Sugars of Cotton Leaves¹

PLASMA AND PHAGOSOME MEMBRANES OF ACANTHAMOEBA CASTELLANII

Biochemical Analysis

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Quantitative analysis of carbohydrates using gas-liquid chromatography

Cited by 61 publications

References 6 publications

Adaptation of Gas‐Liquid Chromatography to Analysis of Certain Sugars of Cotton Leaves1

Adaptation of Gas‐Liquid Chromatography to Analysis of Certain Sugars of Cotton Leaves1

PLASMA AND PHAGOSOME MEMBRANES OF ACANTHAMOEBA CASTELLANII

Biochemical Analysis

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Adaptation of Gas‐Liquid Chromatography to Analysis of Certain Sugars of Cotton Leaves¹

Adaptation of Gas‐Liquid Chromatography to Analysis of Certain Sugars of Cotton Leaves¹