Quantitative analysis of cefazolin sodium in lyophilized powder by infrared spectrophotometry: Green, low cost, fast and effective

Rechelo, Bárbara Saú; Kogawa, Ana Carolina; Salgado, Hérida Regina Nunes

doi:10.1016/j.saa.2018.09.058

Cited by 25 publications

(8 citation statements)

References 20 publications

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“… 18 The FT-IR spectrum of Cef ( Figure 2h ) displayed sharp bands at 3415, 3284, 3143, 3059, 2960, 2862, 1761, 1674, 1600, 1550, 1489, 1282, 1240,1184, 1101, and 1064 cm -1 . 26 The sharp peaks at 3415, 3284, and 1600 cm -1 were related to the vibration of N-H stretching and the bending vibration of Cef.…”

Section: Resultsmentioning

confidence: 98%

Cefazolin-Loaded Double-Shelled Hollow Mesoporous Silica Nanoparticles/Polycaprolactone Nanofiber Composites: A Delivery Vehicle for Regenerative Purposes

et al. 2022

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As important challenges in burn injuries, infections often lead to delayed and incomplete healing. Wound infections with antimicrobial-resistant bacteria are other challenges in the management of wounds. Hence, it can be critical to synthesize scaffolds that are highly potential for loading and delivering antibiotics over long periods. Methods: Double-shelled hollow mesoporous silica nanoparticles (DSH-MSNs) were synthesized and loaded with cefazolin. Cefazolin-loaded DSH-MSNs (Cef*DSH-MSNs) were incorporated into polycaprolactone (PCL) to prepare a nanofiber-mediated drug release system. Their biological properties were assessed through antibacterial activity, cell viability, and qRT-PCR. The morphology and physicochemical properties of the nanoparticles and nanofibers were also characterized. Results: The double-shelled hollow structure of DSH-MSNs demonstrated a high loading capacity of cefazolin (51%). According to in vitro findings, the Cef*DSH-MSNs embedded in polycaprolactone nanofibers (Cef*DSH-MSNs/PCL) provided a slow release for cefazolin. The release of cefazolin from Cef*DSH-MSNs/PCL nanofibers inhibited the growth of Staphylococcus aureus. The high viability rate of human adipose-derived stem cells (hADSCs) in contact with PCL and DSH-MSNs/PCL was indicative of the biocompatibility of nanofibers. Moreover, gene expression results confirmed changes in keratinocyte-related differentiation genes in hADSCs cultured on the DSH-MSNs/PCL nanofibers with the up-regulation of involucrin. Conclusion: The high drug-loading capacity of DSH-MSNs presents these nanoparticles as suitable vehicles for drug delivery. In addition, the use of Cef*DSH-MSNs/PCL can be an effective strategy for regenerative purposes.

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Section: Resultsmentioning

confidence: 98%

Cefazolin-Loaded Double-Shelled Hollow Mesoporous Silica Nanoparticles/Polycaprolactone Nanofiber Composites: A Delivery Vehicle for Regenerative Purposes

et al. 2022

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“…A significantly lower amount of solvents was used compared to previously reported methods for the determination of flavonoids of A. satureioides extracts 6,8 . Most analytical methods are costly and complex, requiring a long period of analysis and excessive amounts of organic solvents 19 . The method proposed, besides reducing the analysis time (8 min), requires the use of low amounts of solvent (around 1.15 mL of methanol/run), generating less toxic waste and alleviating environmental impact.…”

Section: Resultsmentioning

confidence: 99%

A stability‐indicating ultra‐fast liquid chromatography method for the assay of the main flavonoids of Achyrocline satureioides (Marcela) in porcine skin layers and nanoemulsions

Balestrin

Fachel

Koester

et al. 2020

Phytochemical Analysis

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Introduction: Achyrocline satureioides (marcela or macela) is a plant widely used in folk medicine in South America. Recently, there has been increasing interest for the development of skin care products containing A. satureoides extracts, due to its welldocumented antioxidant, antiherpetic, and wound healing properties. Objectives: The present study aimed to develop and validate a yet unexplored stability-indicating and robust ultra-fast liquid chromatography (UFLC) method for the simultaneous quantification of the main flavonoids of A. satureioides in extracts, nanoemulsions, and porcine skin layers. Material and methods: The chromatographic separation of flavonoids quercetin, luteolin, and 3-O-methylquercetin was performed on a Luna C18 analytical column (100 mm × 2.0 mm i.d.; particle size 2.5 μm) using isocratic elution with methanol/phosphoric acid 1% (48:52 v/v) with a flow rate of 0.3 mL/min at 40 C. Results: The method was found to be specific, linear (R > 0.998), precise, accurate, and robust for all flavonoids assayed in A. satureioides extract, nanoemulsions, and porcine ear skin. A low matrix effect was noted for all complex matrices. The stability-indicating UFLC method was evaluated by submitting isolated flavonoids, a mixture of standards, and A. satureioides extract to acidic, alkaline, oxidative, UV-A/UV-C light, and thermal stress conditions. No peaks were found co-eluting with the flavonoids of interest in all matrices. The robustness of the method was confirmed using Plackett-Burman experimental design. Conclusion: The short run time (8 min) and reliability of the method could be useful for the determination of A. satureioides flavonoids in topical product development since extracts of this medicinal plant have been used to treat various skin disorders.

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“…Among the examples, it is possible to mention the quantitative analysis to verify the content of darunavir (13), paracetamol (16), rifaximin (24), secnidazole (25) and enrofloxacin (27) in tablets; content of cefadroxil monohydrate (18) in capsules; content of ceftazidime (10), sodium ampicillin (11), sodium cefuroxime (12), sodium cefotaxime (20), daptomycin (21), sodium ceftriaxone (22), sodium ertapenem (26), sodium cephalothin (28), sodium cefazolin (29), cefepime hydrochloride (30) and vancomycin (31) in lyophilized powder; content of doxycycline (17) and norfloxacin (19) in raw material. The technique by spectrophotometry in the infrared region presents different analysis methods.…”

Section: Practical Reviewmentioning

confidence: 99%

Quantitative analysis of pharmaceutical products by spectrophotometry in the infrared region: a practical review

Kogawa¹,

Salgado²

2023

Drug Anal. Res.

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Quality control in the chemical-pharmaceutical industry to identify and quantify the active ingredient has fundamental importance to guarantee the quality of the final product. Its lack can generate irreparable consequences, and therefore its existence is extremely important. However, currently, in this process there is an ecologically correct need beyond the choice of the ideal method and the ideal conditions. This mini-review contemplates the current technological vision of pharmaceutical analysis through Green Analytical Chemistry (GAC). Green and sustainable methods have a main focus providing economic, environmental and social benefits. An example is spectrophotometry in the infrared region for quantitative purposes. The purpose of this mini-review is to show a practical guide for the quantitative analysis of raw materials and pharmaceutical products by spectrophotometry in the infrared region in order to contribute to a sustainability cycle, where the guarantee of product quality and the analytical awareness about health, time, waste generation, environment and cost coexist. A practical review for quantitative analysis by spectrophotometry in the infrared region was shown. It is useful for routine analysis of pharmaceutical products in general, and it can be used by chemical-pharmaceutical laboratories around the world.

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Quantitative analysis of cefazolin sodium in lyophilized powder by infrared spectrophotometry: Green, low cost, fast and effective

Cited by 25 publications

References 20 publications

Cefazolin-Loaded Double-Shelled Hollow Mesoporous Silica Nanoparticles/Polycaprolactone Nanofiber Composites: A Delivery Vehicle for Regenerative Purposes

Cefazolin-Loaded Double-Shelled Hollow Mesoporous Silica Nanoparticles/Polycaprolactone Nanofiber Composites: A Delivery Vehicle for Regenerative Purposes

A stability‐indicating ultra‐fast liquid chromatography method for the assay of the main flavonoids of Achyrocline satureioides (Marcela) in porcine skin layers and nanoemulsions

Quantitative analysis of pharmaceutical products by spectrophotometry in the infrared region: a practical review

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