Quantitative Analysis of Cell Division and Cell Death in Seminal Root of Rye under Salt Stress

Comparison of effect of salt stress on the cell death in seminal root and lateral root of rye seedlings by the modified TUNEL method

“…In the classical method, sections were used to detect and estimate the degree of the cell death in root tip (Katsuhara, 1997;Katsuhara and Kawasaki, 1996;Ogawa et al, 2006). In the present study, we estimated the cell death in the root tip by the modified TUNEL method, exhaustively.…”

“…In the present study, we estimated the cell death in the root tip by the modified TUNEL method, exhaustively. In our previous study (Ogawa et al, 2006), the cell death in seminal root tip of rye seedlings under some degree of salt stress conditions were also investigated using the classical TUNEL method. The frequency of cell death in the 0 mM, 10 mM NaCl treatments detected by the classical TUNEL method were similar to those detected by the modified TUNEL method in the present study.…”

“…The detection and the observation of cell death by the TUNEL labeling and the quantitation of the cell death frequency were followed by the methods as described previously (Ogawa et al, 2006).…”

“…Katsuhara (1997) reported that DNA fragmentation in the seminal root of barley occurred after one hour of 500 mM NaCl treatment, and the TUNEL-labeled cells were increased with duration of the treatment. Ogawa et al (2006) also investigated the cell death in seminal root tip of rye seedlings under some degree of salt stress conditions by the TUNEL method, quantitatively. Cell death was hardly observed from 0 mM (control) to 100 mM of NaCl treatments for one day.…”

“…However, in previous studies, the change of cell death was investigated only at the seminal root tip, and not at the lateral root. In most of the previous studies, longitudinal resin sections were made and they were stained by TUNEL method to detect the cell death in root tip (Katsuhara, 1997;Katsuhara and Kawasaki, 1996;Ogawa et al, 2006). The advantage of this method is to enable us to observe the cell death using an organ part or tissue at a cellular level on the section.…”