Quantitative analysis of cellular senescence phenotypes using an imaging cytometer

Udono, Miyako; Kadooka, Keishi; Yamashita, Shuntaro; Katakura, Yoshinori

doi:10.1016/j.ymeth.2012.02.012

Cited by 20 publications

(21 citation statements)

References 14 publications

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“…IL-6 plus soluble IL-6Ra treatment of young TIG3 cells (passage #33) resulted in growth arrest and senescence-associatedb-galactosidase activity, mimicking a p53/ROS driven DNA damage response. STAT3 was similarly described to be essential for both the early ROS increase, as well as the later senescence-associated-b-galactosidase activity, through STAT3-regulated soluble factor insulinlike growth factor binding protein 5 in both human fibroblasts (Udono et al, 2012) and vascular endothelial cells (Kim et al, 2007).…”

Section: Cell Senescencementioning

confidence: 99%

Targeting Janus Kinases and Signal Transducer and Activator of Transcription 3 to Treat Inflammation, Fibrosis, and Cancer: Rationale, Progress, and Caution

et al. 2020

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Section: Cell Senescencementioning

confidence: 99%

Targeting Janus Kinases and Signal Transducer and Activator of Transcription 3 to Treat Inflammation, Fibrosis, and Cancer: Rationale, Progress, and Caution

et al. 2020

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“…In that case IL-6/STAT3 may trigger induction or reinforce the senescence process of HUVECs by activating the IGFBP5 gene. Recently, another human fibroblast, TIG1, derived from human female fetal lung, were shown to be induced to senescence by IL-6 64 . It is possible that the senescence-inducing circuit involving IL-6-STAT3-IGFBP5 may not be limited to TIG3 cells.…”

Section: Il-6/stat3 Regulates Multiple Processes Ranging From Prematumentioning

confidence: 99%

IL-6-STAT3 signaling and premature senescence

et al. 2013

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Cytokines play several roles in developing and/or reinforcing premature cellular senescence of young cells. One such cytokine, interleukin-6 (IL-6), regulates senescence in some systems in addition to its known functions of immune regulation and promotion of tumorigenesis. In this review, we describe recent advances in studies on the roles of IL-6 and its downstream signal transducer and activator of transcription 3 (STAT3) in regulating premature cellular senescence. IL-6/sIL-6Rα stimulation forms a senescence-inducing circuit involving the STAT3-insulin-like growth factor-binding protein 5 (IGFBP5) as a key axis triggering and reinforcing component in human fibroblasts. We describe how cytokines regulate the process of senescence by activating STAT3 in one system and anti-senescence or tumorigenesis in other systems. The roles of other STAT members in premature senescence also will be discussed to show the multiple mechanisms leading to cytokine-induced senescence.

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“…SH-SY5Y cells were fixed with 4% paraformaldehyde for 30 min and then blocked with a blocking buffer (1 × PBS, 1% bovine serum albumin, 5% FBS, 0.2% Triron X-100) for 1 h. Next, cells were stained with a Milli-Mark FluoroPan Neuronal Marker (Merk Millipore, Billerica, MA, USA) at room temperature for 2 h. Neurite growth was quantitatively determined using a Neurite Outgrowth Assay kit (Merk Millipore), according to the manufacturer's instructions [16].…”

Section: Quantitative Evaluation Of Neurite Growthmentioning

confidence: 99%

Mechanisms of carnosine-induced activation of neuronal cells

Yamashita

Sato

Mutoh

et al. 2018

Bioscience, Biotechnology, and Biochemistry

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Carnosine (β-Ala-l-His), an imidazole dipeptide, is known to have many functions. Recently, we demonstrated in a double-blind randomized controlled trial that carnosine is capable of preserving cognitive function in elderly people. In the current study, we assessed the ability of carnosine to activate the brain, and we tried to clarify the molecular mechanisms behind this activation. Our results demonstrate that carnosine permeates the blood brain barrier and activates glial cells within the brain, causing them to secrete neurotrophins, including BDNF and NGF. These results point to a novel mechanism of carnosine-induced neuronal activation. Our results suggest that carnosine should be recognized as a functional food factor that helps achieve anti-brain aging.

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Quantitative analysis of cellular senescence phenotypes using an imaging cytometer

Cited by 20 publications

References 14 publications

Targeting Janus Kinases and Signal Transducer and Activator of Transcription 3 to Treat Inflammation, Fibrosis, and Cancer: Rationale, Progress, and Caution

Targeting Janus Kinases and Signal Transducer and Activator of Transcription 3 to Treat Inflammation, Fibrosis, and Cancer: Rationale, Progress, and Caution

IL-6-STAT3 signaling and premature senescence

Mechanisms of carnosine-induced activation of neuronal cells

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