Quantitative analysis of differentially expressed milk fat globule membrane proteins between donkey and bovine colostrum based on high-performance liquid chromatography with tandem mass spectrometry proteomics

Liang, Xiaona; Zheng, Kexin; Song, Wanying; Yu, Haikun; Zhang, Xiumin; Yue, Xiqing; Li, Qilong

doi:10.3168/jds.2021-20471

Cited by 16 publications

(13 citation statements)

References 53 publications

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“…Furthermore, differences in the proteomes of milk fat globules in donkey and cow milk have been studied. 39 79 MFGM proteins and 195 MFGM proteins were identified in donkey and bovine colostrum, respectively. Among these, 71 proteins were common to both, 108 were specific to donkey colostrum, and 124 were specific to bovine colostrum.…”

Section: Advances In Donkey Milkmentioning

confidence: 99%

Proteomics and Peptidomics As a Tool to Compare the Proteins and Endogenous Peptides in Human, Cow, and Donkey Milk

Ning,

Yang,

Liu

et al. 2023

J. Agric. Food Chem.

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Cow’s milk is the most widely used ingredient in infant formulas. However, its specific protein composition can cause allergic reactions. Finding alternatives to replace cow’s milk and fill the nutritional gap with human milk is essential for the health of infants. Proteomic and peptidomic techniques have supported the elucidation of milk’s nutritional ingredients. Recently, omics approaches have attracted increasing interest in the investigation of milk because of their high throughput, precision, sensitivity, and reproducibility. This review offers a significant overview of recent developments in proteomics and peptidomics used to study the differences in human, cow, and donkey milk. All three types of milks were identified to have critical biological functions in human health, particularly in infants. Donkey milk proteins were closer in composition to human milk, were less likely to cause allergic reactions, and may be developed as novel raw materials for formula milk powders.

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Section: Advances In Donkey Milkmentioning

confidence: 99%

Proteomics and Peptidomics As a Tool to Compare the Proteins and Endogenous Peptides in Human, Cow, and Donkey Milk

Ning,

Yang,

Liu

et al. 2023

J. Agric. Food Chem.

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“…The identification of proteins, metabolites, and free fatty acids in donkey colostrum has been carried out. [21][22][23][24][25][26] Guan et al conducted a comparison of glycoproteins, glycopeptides, and glycosylation sites in whey proteins in donkey colostrum and mature milk. During lactation, a total of 164 whey proteins from donkey milk were analysed, revealing the presence of 946 N-glycopeptides.…”

Section: Introductionmentioning

confidence: 99%

Characterization and biological function analysis of endogenous peptides derived from donkey colostrum proteins

Ning,

Li,

Chen

et al. 2023

Food Funct.

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“…However, understanding of their potential medical values is still limited. In addition, milk is different from other body fluids (such as plasma), as well as its composition is complex, and contains a mass of small molecules of milk proteins and milk fat ( 21 , 22 ). Some of the small molecules are similar in size to exosomes, which may lead to low concentration and purity of milk-derived exosomes after isolation ( 22 , 23 ).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, milk is different from other body fluids (such as plasma), as well as its composition is complex, and contains a mass of small molecules of milk proteins and milk fat ( 21 , 22 ). Some of the small molecules are similar in size to exosomes, which may lead to low concentration and purity of milk-derived exosomes after isolation ( 22 , 23 ). To date, there is no recognized separation method that can obtain high purity, concentration, and bioactivity of milk-derived exosomes ( 24 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of plasma-derived exosomes from the normal and thin Bactrian camels on hepatocellular carcinoma and their differences at transcriptome and proteomics levels

Yao

Yu²,

Luo³

et al. 2023

Front. Oncol.

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BackgroundHepatocellular carcinoma (HCC) is a common malignant primary tumor. Bactrian camels have high economic and social values, but their potential medical value has not been studied. This study aimed to investigate the effects of Bactrian camel plasma-derived exosomes on HCC.MethodsPlasma was obtained from thin and normal Bactrian camels, and used to isolate exosomes by ultracentrifugation. The exosomes were then characterized by transmission electron microscopy and Nano particle tracking analyzer. In vivo imaging of nude mice and hematoxylin eosin (HE) staining of liver tissues were used to explore the effects of the exosomes on tumor growth. Finally, the differences of the two exosomes were further analyzed using small RNA sequencing and proteomics.ResultsIn vivo imaging and HE staining showed that no significant differences were found in fluorescence value and liver tissue morphology between the control mice and the mice treated with the exosomes from thin Bactrian camels; while the fluorescence value and the live histology changes were alleviated in the mice with the exosomes from normal Bactrian camels. After sequencing and proteomic analysis, 40 differentially expressed miRNAs (DE-miRNAs, 15 down-regulated and 25 up-regulated) and 172 differentially expressed proteins (DEPs, 77 up-regulated and 95 down-regulated) were identified in the plasma-derived exosomes from normal Bactrian camels. These identified DE-miRNAs and DEPs were significantly enriched in many signaling pathways.ConclusionsNormal Bactrian camel plasma-derived exosomes may inhibit the growth of HCC cells through regulating pathways of Ras, Ras-Association Proximate 1 (Rap1), phosphoinositide 3-kinase-protein kinase B (PI3K-Akt), mitogen-activated protein kinase (MAPK), adenosine monophosphate-activated protein kinase (AMPK), and canonical Wnt signaling pathways.

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Quantitative analysis of differentially expressed milk fat globule membrane proteins between donkey and bovine colostrum based on high-performance liquid chromatography with tandem mass spectrometry proteomics

Cited by 16 publications

References 53 publications

Proteomics and Peptidomics As a Tool to Compare the Proteins and Endogenous Peptides in Human, Cow, and Donkey Milk

Proteomics and Peptidomics As a Tool to Compare the Proteins and Endogenous Peptides in Human, Cow, and Donkey Milk

Characterization and biological function analysis of endogenous peptides derived from donkey colostrum proteins

Effects of plasma-derived exosomes from the normal and thin Bactrian camels on hepatocellular carcinoma and their differences at transcriptome and proteomics levels

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