Quantitative analysis of fibrosis formation on the microcapsule surface with the use of picro‐sirius red staining, polarized light microscopy, and digital image analysis

Zhang, Hua'an; Sun, Lin; Wang, Wei; Ma, Xiaojun

doi:10.1002/jbm.a.30491

Cited by 42 publications

(39 citation statements)

References 26 publications

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“…Under polarised light, yellow and red are thought to signify collagen type I-like fibres, while green suggests collagen type III-like fibres [16][17][18] However, it is not uncommon for foreign body giant cells to form around porous inert materials who have an irregular morphology with a pore size less than 20µm 4,6,36 .…”

Section: Discussionmentioning

confidence: 99%

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Structural variants of biodegradable polyesterurethane in vivo evoke a cellular and angiogenic response that is dictated by architecture

et al. 2009

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The aim of this study was to investigate an in-vivo tissue response to a biodegradable polyesterurethane, specifically the cellular and angiogenic response evoked by varying implant architectures in a subcutaneous rabbit implant model. A synthetic biodegradable polyesterurethane was synthesised and processed into three different configurations; a non-porous film, a porous mesh and a porous membrane. Glutaraldehyde cross-linked bovine pericardium was used as a control. Sterile polyesterurethane and control samples were implanted subcutaneously in six rabbits, (n=12). The rabbits were sacrificed at 21 and 63 days and the implant sites were sectioned and histologically stained using haemotoxylin and eosin (H&E), Masson's trichrome, picosirius red and immunostain CD31. The tissue-implant interface thickness was measured from the H&E slides.Stereological techniques were used to quantify the tissue reaction at each time point that included volume fraction of inflammatory cells, fibroblasts, fibrocytes, collagen and the degree of vascularisation. Stereological analysis inferred that porous scaffolds with regular topography are better tolerated in-vivo compared to non-porous scaffolds, while increasing scaffold porosity promotes angiogenesis and cellular infiltration. The results suggest that this biodegradable polyesterurethane is better tolerated in-vivo than the control and that structural variants of biodegradable polyesterurethane in-vivo evoke a cellular and angiogenic response that is dictated by architecture.

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Section: Discussionmentioning

confidence: 99%

“…The formalin fixed tissues were dehydrated and permeated with paraffin in automatic tissue processor (Leica ASP300), blocked (LeicaEG1150H and EG1150C) in paraffin and were denoted collagen type I-like whilst green was denoted collagen type III-like [16][17][18] .…”

Section: Histopathology and Stainingmentioning

confidence: 99%

Structural variants of biodegradable polyesterurethane in vivo evoke a cellular and angiogenic response that is dictated by architecture

et al. 2009

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“…The histological cuts colored using Pricosirius and observed by polarized light are an adequate method to identify mainly types I and III from the collagen types. It was used a imagecapturing process via video and quantification via coloring, by means of a computer software as shown in other studies (20,28) . The analysis carried out by WOLWACZ et al (27) showed an average percent collagen area in the patients, 33% smaller than in the controls.…”

Section: Discussionmentioning

confidence: 99%

Quantitative and qualitative analysis of collagen types in the fascia transversalis of inguinal hernia patients

Meyer¹,

Berger²,

Monteiro³

et al. 2007

Arq. Gastroenterol.

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-Background -Inguinal hernia is the second most common surgical case in our field. The anatomical factors alone are not enough to explain the inguinal hernia. Studies show changes in the proportion and quantity of collagen fibers in the developing of inguinal hernia. The greater production of collagen type III compared to the type I could justify the thinning of the fascia transversalis and its weakness. Aim -To determine the quantitative and qualitative changes of collagen in the fascia transversalis in inguinal hernia patients and compare them to findings from corpses without inguinal hernia. Method -Prospective case-control study based on the biopsy of fascia transversalis of 27 patients and 24 corpses. The technique used was hematoxylin-eosin and picrosirius colorimetry. Results -The medium percent area of collagen (types I + III) and collagen type I, in both groups, show no statistic difference. The quantity of collagen type III was greater in the patients. Patients classified with Nyhus IIIa presented greater quantity of collagen type III. Conclusion -There is no significant difference in the quantity of collagen in the fascia transversalis of patients compared to the controls. An increase in the quantity of collagen type III was found in patients with inguinal hernia and a greater quantity in those patients classified with Nyhus IIIa. HEADINGS -Hernia, inguinal. Fascia. Collagen.

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“…According to a previously described method (Zhang et al, 2006), lung-tissue paraffin sections were conventionally dewaxed and replaced with water. A saturated Sirius red-picric acid solution (Sigma-Aldrich) was used for the staining.…”

Section: Picro-sirius Red Stainingmentioning

confidence: 99%

Effects of angiotensin II intervention on MMP-2, MMP-9, TIMP-1, and collagen expression in rats with pulmonary hypertension

Wang¹,

Shi²,

Li³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. This study investigated the effects of angiotensin II (AngII) intervention, using captopril and losartan, on the expression of matrix metalloproteinase-2 (MMP-2), MMP-9, tissue inhibitor of metalloproteinase-1 (TIMP-1), and collagen in rats with pulmonary hypertension, in an effort to understand mechanisms underlying pulmonary vascular remodeling. A total of 40 male Sprague-Dawley rats were randomly divided into normal group, model group, captopril group, and losartan group. After 5 weeks, the mean pulmonary arterial pressure (mPAP), right ventricular index, and neointima formation in each group were determined. Immunohistochemical analysis was performed to determine the degree of pulmonary arterial muscularization as well as MMP-2, MMP-9, and TIMP-1 protein expression in lung tissue. Real-time fluorescent quantitative PCR was 1707-1717 (2015) used to detect MMP2, MMP9, TIMP1, COL1A1, and COL4A1 mRNA expression. Picro-sirius red staining was performed to detect collagen protein expression. Neointima formation was observed in the model group. Moreover, the mPAP, right ventricular index, degree of arterial muscularization, and collagen deposition, as well as mRNA and protein expression of MMP2, MMP9, and TIMP1 were significantly higher than those in the other groups (P < 0.05). The mPAP, right ventricular index, degree of arterial muscularization, and mRNA and protein expression in the captopril and losartan groups were significantly decreased compared with those of the model group (P < 0.05). AngII regulates MMP-2, MMP-9, and TIMP-1 expression and affects collagen deposition. Thus, this hormone is involved in pulmonary vascular remodeling, indicating a possible mechanism that can be targeted in pulmonary hypertension intervention.

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Quantitative analysis of fibrosis formation on the microcapsule surface with the use of picro‐sirius red staining, polarized light microscopy, and digital image analysis

Cited by 42 publications

References 26 publications

Structural variants of biodegradable polyesterurethane in vivo evoke a cellular and angiogenic response that is dictated by architecture

Structural variants of biodegradable polyesterurethane in vivo evoke a cellular and angiogenic response that is dictated by architecture

Quantitative and qualitative analysis of collagen types in the fascia transversalis of inguinal hernia patients

Effects of angiotensin II intervention on MMP-2, MMP-9, TIMP-1, and collagen expression in rats with pulmonary hypertension

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