Quantitative analysis of interactive behavior of mitochondria and lysosomes using structured illumination microscopy

Chen, Qixin; Shao, Xintian; Hao, Mingang; Fang, Hongbao; Guan, Ruilin; Tian, Ze; Wen, Feng; Wang, Chenran; Ji, Liang‐Nian; Chao, Hui; Guan, Jun‐Lin; Diao, Jiajie

doi:10.1016/j.biomaterials.2020.120059

Cited by 80 publications

(46 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Morphine also induces macrophage apoptosis in other ways. The apoptosis of macrophages is related to organelles such as mitochondria ( Chen et al, 2020 ; Wang K. et al, 2020 ; Chen et al, 2021 ; Liu et al, 2021a ; Liu et al, 2021b ) ( Figure 1 ). Mitochondria are not only the main sites for intracellular oxidative phosphorylation and the formation of ATP ( Wei et al, 2022 ), but also the sites for the production of ROS ( Forte et al, 2021 ; Wang et al, 2021 ).…”

Section: Opioids Affect the Apoptosis Of Macrophagesmentioning

confidence: 99%

Opioids Regulate the Immune System: Focusing on Macrophages and Their Organelles

et al. 2022

View full text Add to dashboard Cite

Opioids are the most widely used analgesics and therefore have often been the focus of pharmacological research. Macrophages are the most plastic cells in the hematopoietic system. They show great functional diversity in various organism tissues and are an important consideration for the study of phagocytosis, cellular immunity, and molecular immunology. The expression of opioid receptors in macrophages indicates that opioid drugs act on macrophages and regulate their functions. This article reviewed the collection of research on effects of opioids on macrophage function. Studies show that opioids, both endogenous and exogenous, can affect the function of macrophages, effecting their proliferation, chemotaxis, transport, phagocytosis, expression of cytokines and chemokine receptors, synthesis and secretion of cytokines, polarization, and apoptosis. Many of these effects are closely associated with mitochondrial function and functions of other organelles in macrophages. Therefore, in depth research into effects of opioids on macrophage organelles may lead to some interesting new discoveries. In view of the important role of macrophages in HIV infection and tumor progression, this review also discusses effects of opioids on macrophages in these two pathological conditions.

show abstract

Section: Opioids Affect the Apoptosis Of Macrophagesmentioning

confidence: 99%

Opioids Regulate the Immune System: Focusing on Macrophages and Their Organelles

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Mitophagy is a process by which cells remove and degrade damaged mitochondria, and its typical feature is the overlap of lysosomes and mitochondria. ( Chen et al, 2020b ; Chen Q. et al, 2021 ) After clarifying that 3a can damage mitochondria, we further studied whether drug-induced mitochondrial damage is involved in the mitophagy pathway.…”

Section: Resultsmentioning

confidence: 99%

Efficient Synthesis of 2,3′-Spirobi (Indolin)-2′-Ones and Preliminary Evaluation of Their Damage to Mitochondria in HeLa Cells

Sun

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

A novel formal (4 + 1) annulation between N-(o-chloromethyl)aryl amides and 3-chlorooxindoles through in situ generated aza-ortho-QMs with 3-chlorooxindoles is reported for the synthesis of a series of 2,3′-spirobi (indolin)-2′-ones in high yields. Under structured illumination microscopy, compound 3a is found to change the mitochondrial morphology and induce mitophagy pathway, which might then trigger mitophagy in cancer cells.

show abstract

“…Super-resolution microscope technology is advanced in observing small-scale intracellular components (such as protein [117,118], lysosomal [119,120], bacteria [121,122]). However, there are few attempts to use SIM and STEDM for micromanipulation because the current robot-aided manipulation tools can only handle objects at as low as sub-micrometers.…”

Section: Super-resolution Microscopymentioning

confidence: 99%

Advanced Biological Imaging for Intracellular Micromanipulation: Methods and Applications

et al. 2020

View full text Add to dashboard Cite

Intracellular micromanipulation assisted by robotic systems has valuable applications in biomedical research, such as genetic diagnosis and genome-editing tasks. However, current studies suffer from a low success rate and a large operation damage because of insufficient information on the operation information of targeted specimens. The complexity of the intracellular environment causes difficulties in visualizing manipulation tools and specimens. This review summarizes and analyzes the current development of advanced biological imaging sampling and computational processing methods in intracellular micromanipulation applications. It also discusses the related limitations and future extension, providing an important reference about this field.

show abstract

Quantitative analysis of interactive behavior of mitochondria and lysosomes using structured illumination microscopy

Cited by 80 publications

References 39 publications

Opioids Regulate the Immune System: Focusing on Macrophages and Their Organelles

Opioids Regulate the Immune System: Focusing on Macrophages and Their Organelles

Efficient Synthesis of 2,3′-Spirobi (Indolin)-2′-Ones and Preliminary Evaluation of Their Damage to Mitochondria in HeLa Cells

Advanced Biological Imaging for Intracellular Micromanipulation: Methods and Applications

Contact Info

Product

Resources

About