Quantitative analysis of mitochondrial DNAs in macaque embryos reprogrammed by rabbit oocytes

Yang, Chao-Tung; Kou, Zhao Hui; Wang, Kai; Jiang, Yan; Mao, Wen Wei; Sun, Qing; Sheng, Hua; Chen, Da Yuan

doi:10.1530/rep.1.00088

Cited by 44 publications

(40 citation statements)

References 19 publications

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“…reconstructed embryos (Hiendleder et al 2003, Yang et al 2004, Burgstaller et al 2007. These organelles are involved in cellular metabolism with ATP production, apoptosis, regulation of calcium and cellular aging (Wang et al 2009b), so an inefficient nucleo-cytoplasmic communication for the regulation of mtDNA transcription and replication can lead to failure in embryonic development.…”

Section: Effect Of Iscnt and Aggregation On In Vitro Development Of Dmentioning

confidence: 99%

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Moro¹,

Hiriart²,

Buemo³

et al. 2015

Reproduction

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The aim of this study was to evaluate the capacity of domestic cat (Dc, Felis silvestris) oocytes to reprogram the nucleus of cheetah (Ch, Acinonyx jubatus) cells by interspecies SCNT (iSCNT), by using embryo aggregation. Dc oocytes were in vitro matured and subjected to zona pellucida free (ZP-free) SCNT or iSCNT, depending on whether the nucleus donor cell was of Dc or Ch respectively. ZP-free reconstructed embryos were then cultured in microwells individually (Dc1X and Ch1X groups) or in couples (Dc2X and Ch2X groups). Embryo aggregation improved in vitro development obtaining 27.4, 47.7, 16.7 and 28.3% of blastocyst rates in the Dc1X, Dc2X, Ch1X and Ch2X groups, respectively (P!0.05). Moreover, aggregation improved the morphological quality of blastocysts from the Dc2X over the Dc1X group. Gene expression analysis revealed that Ch1X and Ch2X blastocysts had significantly lower relative expression of OCT4, CDX2 and NANOG than the Dc1X, Dc2X and IVF control groups. The OCT4, NANOG, SOX2 and CDX2 genes were overexpressed in Dc1X blastocysts, but the relative expression of these four genes decreased in the Dc2X, reaching similar relative levels to those of Dc IVF blastocysts. In conclusion, Ch blastocysts were produced using Dc oocytes, but with lower relative expression of pluripotent and trophoblastic genes, indicating that nuclear reprogramming could be still incomplete. Despite this, embryo aggregation improved the development of Ch and Dc embryos, and normalized Dc gene expression, which suggests that this strategy could improve full-term developmental efficiency of cat and feline iSCNT embryos.Reproduction (2015) 150 1-10

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Section: Effect Of Iscnt and Aggregation On In Vitro Development Of Dmentioning

confidence: 99%

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Moro¹,

Hiriart²,

Buemo³

et al. 2015

Reproduction

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“…It has been detected in bovine embryos derived by both intraspecific NT (Steinborn et al 1998a;Do et al 2002) and interspecific NT (Meirelles et al 2001) although not in all cases (Meirelles et al 2001;Takedaet al 2003). Additionally, D-mtDNA was detected in caprine embryos derived by interspecific NT ( Jiang et al 2004) and in embryos derived by cross-species NT (Chang et al 2003;Yang et al 2003). Again, the levels of D-mtDNA detected in NT-derived embryos are equally variable.…”

mentioning

confidence: 93%

Aberrant Nucleo-cytoplasmic Cross-Talk Results in Donor Cell mtDNA Persistence in Cloned Embryos

et al. 2006

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Mitochondrial DNA is an extranuclear genome normally maternally inherited through the oocyte. However, the use of nuclear transfer can result in both donor cell and recipient oocyte mitochondrial DNA persisting through to blastocyst and being transmitted to the offspring. The degree of donor mitochondrial DNA transmission appears to be random and currently no evidence exists to explain this phenomenon. To determine whether this is a dilution factor or directly related to the transcriptional status of the donor cell in respect of mitochondrial DNA transcription factors, we have generated sheep nuclear transfer embryos using donor cells: (1) possessing their full mitochondrial DNA complement, (2) those partially depleted, and (3) those depleted but containing residual levels. For each donor type, donor mitochondrial DNA persisted in some blastocysts. It is evident from the donor cells used that nuclearencoded mitochondrial DNA transcription and replication factors persist even after mitochondrial DNA depletion, as do transcripts for some of the mitochondrial-encoded genes. These cells are therefore still programmed to drive mitochondrial DNA replication and transcription. In nuclear transfer-derived embryos, we have observed the persistence of these nuclear-encoded mitochondrial DNA transcription and replication factors but not in those embryos generated through in vitro fertilization. Consequently, nucleo-mitochondrial interaction following nuclear transfer is out of sequence as the onset of mitochondrial replication is a postimplantation event.

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“…In iSCNT embryos, the copy numbers of D-mtDNA were found to be constant from the 1-cell to the 8-cell stage in water buffalo-bovine (Fig. 4) [60], sheepbovine [61], cat-bovine [62], goat-sheep [63], and macaque-rabbit [64] arrested embryos. Similarly, in pronuclear bovine zygotes injected with (buffalo or murine) mitochondria illustrated that exogenous interspecies/intergeneric mtDNA injected into bovine oocytes were not selectively destroyed; indeed, they were maintained throughout early development (until the blastocyst stage) (Fig.…”

Section: Fate Of Interspecies or Intergeneric Mtdna In Ooplasmmentioning

confidence: 99%

Mitochondrial DNA transmission and confounding mitochondrial influences in cloned cattle and pigs

Takeda

2013

Reprod Medicine & Biology

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Although somatic cell nuclear transfer (SCNT) is a powerful tool for production of cloned animals, SCNT embryos generally have low developmental competency and many abnormalities. The interaction between the donor nucleus and the enucleated ooplasm plays an important role in early embryonic development, but the underlying mechanisms that negatively impact developmental competency remain unclear. Mitochondria have a broad range of critical functions in cellular energy supply, cell signaling, and programmed cell death; thus, affect embryonic and fetal development. This review focuses on mitochondrial considerations influencing SCNT techniques in farm animals. Donor somatic cell mitochondrial DNA (mtDNA) can be transmitted through what has been considered a ''bottleneck'' in mitochondrial genetics via the SCNT maternal lineage. This indicates that donor somatic cell mitochondria have a role in the reconstructed cytoplasm. However, foreign somatic cell mitochondria may affect the early development of SCNT embryos. Nuclear-mitochondrial interactions in interspecies/intergeneric SCNT (iSCNT) result in severe problems. A major biological selective pressure exists against survival of exogenous mtDNA in iSCNT. Yet, mtDNA differences in SCNT animals did not reflect transfer of proteomic components following proteomic analysis. Further study of nuclear-cytoplasmic interactions is needed to illuminate key developmental characteristics of SCNT animals associated with mitochondrial biology.

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Quantitative analysis of mitochondrial DNAs in macaque embryos reprogrammed by rabbit oocytes

Cited by 44 publications

References 19 publications

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Aberrant Nucleo-cytoplasmic Cross-Talk Results in Donor Cell mtDNA Persistence in Cloned Embryos

Mitochondrial DNA transmission and confounding mitochondrial influences in cloned cattle and pigs

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