Quantitative analysis of multicomponents by a single marker and quality evaluation of Venenum Bufonis from different geographical origins

Cui, Keke; Cao, Yueting; Shao, Jia‐feng; Lu, Zheng-Yu; Wang, Longhu

doi:10.1002/bmc.4555

Cited by 9 publications

(5 citation statements)

References 20 publications

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“…Methanol extracts of samples were prepared as described above. The bufogenins concentration were determined by the method established previously 26 . The chromatographic analysis was primarily performed by an Agilent 1200 system (Agilent Technologies, Tokyo).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The impact of multiple climatic and geographic factors on the chemical defences of Asian toads (Bufo gargarizans Cantor)

Cao¹,

Cui²,

Pan³

et al. 2019

Sci Rep

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Chemical defences are widespread in nature, yet we know little about whether and how climatic and geographic factors affect their evolution. In this study, we investigated the natural variation in the concentration and composition of the main bufogenin toxin in adult Asian toads (Bufo gargarizans Cantor) captured in twenty-two regions. Moreover, we explored the relative importance of eight climatic factors (average temperature, maximum temperature, minimum temperature, average relative humidity, 20–20 time precipitation, maximum continuous precipitation, maximum ground temperature, and minimum ground temperature) in regulating toxin production. We found that compared to toads captured from central and southwestern China, toads from eastern China secreted higher concentrations of cinobufagin (CBG) and resibufogenin (RBG) but lower concentrations of telocinobufagin (TBG) and cinobufotalin (CFL). All 8 climatic variables had significant effects on bufogenin production (ri>0.5), while the plastic response of bufogenin toxin to various climate factors was highly variable. The most important climatic driver of total bufogenin production was precipitation: the bufogenin concentration increased with increasing precipitation. This study indicated that the evolution of phenotypic plasticity in chemical defences may depend at least partly on the geographic variation of defensive toxins and their climatic context.

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Section: Methodsmentioning

confidence: 99%

“…Among them, gamabufotalin (GB), telocinobufagin (TBG), bufotalin (BFL), cinobufotalin (CFL), bufalin (BL), cinobufagin (CBG) and resibufogenin (RBG) (Fig. 1) are the dominant components, representing more than 80% of the total amount of bufagenins 26 . These compounds are considered major contributors to the role of toxins in defence 27 .…”

Section: Introductionmentioning

confidence: 99%

The impact of multiple climatic and geographic factors on the chemical defences of Asian toads (Bufo gargarizans Cantor)

Cao¹,

Cui²,

Pan³

et al. 2019

Sci Rep

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show abstract

“…A component of a given herbal medicine that is stable, inexpensive, easily available, pharmacologically active and easy to separate under conventional chromatographic conditions is an ideal candidate for a marker compound [29]. In this study, rutin, the most abundant and pharmacologically active component of M. stenopetala, was selected as single marker for the quantification of GLSs and phenolics in moringa leaves.…”

Section: Quality Evaluation Of Moringa Samples By Esm and Qamsmentioning

confidence: 99%

Fast RP-HPLC Method for Analysis of Glucosinolates and Phenolics in Moringa stenopetala Leaf Powder Preparations

et al. 2023

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Moringa stenopetala (M. stenopetala) leaf powder is extensively used as food, for preparation of nutraceuticals and herbal medicines in many developing countries. Glucosinolates and phenolics are known to be important bioactive constituents of these preparations. In this study, a selective and fast RP-HPLC method was developed and validated for simultaneous determination of intact glucosinolates and phenolics in M. stenopetala leaf powders. The developed method was linear with a coefficient of determination (r 2 ) ≥ 0.998, precision ≤1.5% and recovery values close to 100%. The method was also sensitive with detection and quantification limits below 1.5 µg/mL and 5 µg/mL, respectively. Besides the use of external standards, quantitative analysis of multicomponents by a single marker (QAMS) was developed and applied to commercial moringa samples. The correlation coefficient (r ≥ 0.9993) between the QAMS and the external standard method proved the consistency and the strength of the relationship between the two methods. Rutin was used as single marker for the determination of 5 bioactive components in M. stenopetala leaf samples. Furthermore, a radar plot was used to differentiate samples collected from different geographical origin. Analysis results of the commercial samples revealed that rutin was found to be the constituent with the highest concentration (varying from 6.6 mg/g to 18.8 mg/g) among the phenolics while glucomoringin (0.2 mg/g to 4.2 mg/g) was the most common glucosinolate.

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“…Recently, QAMS has been extensively applied for the quality analysis of TCM, and is considered to be a feasible alternative to ESM [15][16][17]. It is gradually included in the official pharmacopoeia of an increasing number of countries such as China, India, USA, and the countries in Europe [18].…”

Section: Introductionmentioning

confidence: 99%

Rapid Analysis of Four Alkaloids in Uncaria rhynchophylla by Core-Shell Column HPLC and Quantitative Analysis of Multi-Components by Single Marker (QAMS)

Lin¹,

Zhou²,

Han³

et al. 2023

Phyton

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As a traditional herbal medicine, the major alkaloids in Uncaria rhynchophylla have been proven to have blood pressure-lowering and sedative effects. It is essential to develop an effective method for the determination of the major alkaloids in U. rhynchophylla. In this research, a rapid quantitative analysis involving multi-components analysis by a single marker strategy coupled with core-shell column HPLC was adopted to analyse four alkaloids (corynoxeine, isocorynoxeine, isorhynchophylline, rhynchophylline) in U. rhynchophylla. Isorhynchophylline was selected as the internal reference substance, the content of which was determined by the traditional external standard method. Relative correction factors (RCF) between isorhynchophylline and the other three alkaloids were calculated respectively. The results showed that the QAMS method had good robustness under different HPLC instruments. Nineteen batches of U. rhynchophylla were tested. No significant difference was observed between the results by QAMS and EMS (Correlation coefficient > 0.99, p > 0.05). The QAMS method could be employed as a rapid, effective technique for the quality control of U. rhynchophylla.

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Quantitative analysis of multicomponents by a single marker and quality evaluation of Venenum Bufonis from different geographical origins

Cited by 9 publications

References 20 publications

The impact of multiple climatic and geographic factors on the chemical defences of Asian toads (Bufo gargarizans Cantor)

The impact of multiple climatic and geographic factors on the chemical defences of Asian toads (Bufo gargarizans Cantor)

Fast RP-HPLC Method for Analysis of Glucosinolates and Phenolics in Moringa stenopetala Leaf Powder Preparations

Rapid Analysis of Four Alkaloids in Uncaria rhynchophylla by Core-Shell Column HPLC and Quantitative Analysis of Multi-Components by Single Marker (QAMS)

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