2021
DOI: 10.1096/fj.202100899r
|View full text |Cite
|
Sign up to set email alerts
|

Quantitative analysis of neuronal mitochondrial movement reveals patterns resulting from neurotoxicity of rotenone and 6‐hydroxydopamine

Abstract: Alterations in mitochondrial dynamics, including their intracellular trafficking, are common early manifestations of neuronal degeneration. However, current methodologies used to study mitochondrial trafficking events rely on parameters that are primarily altered in later stages of neurodegeneration. Our objective was to establish a reliable applied statistical analysis to detect early alterations in neuronal mitochondrial trafficking. We propose a novel quantitative analysis of mitochondria trajectories based… Show more

Help me understand this report
View preprint versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
3
0

Year Published

2022
2022
2024
2024

Publication Types

Select...
3
1

Relationship

2
2

Authors

Journals

citations
Cited by 4 publications
(5 citation statements)
references
References 83 publications
(129 reference statements)
0
3
0
Order By: Relevance
“…Moreover, 6-hydroxydopamine (6-OHDA) and rotenone have been used to develop PD models [ 15 ]. In this work, differentiated SH-SY5Y cells were treated for different time points and with distinct concentrations of 6-OHDA and rotenone, which were previously shown to affect mitochondrial movement in these cells [ 16 ]. Experimental biological analyses of cell and mitochondrial function parameters were then assessed.…”
Section: Introductionmentioning
confidence: 99%
“…Moreover, 6-hydroxydopamine (6-OHDA) and rotenone have been used to develop PD models [ 15 ]. In this work, differentiated SH-SY5Y cells were treated for different time points and with distinct concentrations of 6-OHDA and rotenone, which were previously shown to affect mitochondrial movement in these cells [ 16 ]. Experimental biological analyses of cell and mitochondrial function parameters were then assessed.…”
Section: Introductionmentioning
confidence: 99%
“…The package was applied to study neuronal mitochondrial trafficking in neuroblastoma cell lines ( Simões et al 2021 ). In this study, the researchers exposed the cells to mitochondrial toxins and recorded the mitochondrial trajectories using TIRF microscopy (see Fig.…”
Section: Validation and Resultsmentioning
confidence: 99%
“…In (F), the change in a set of four features from TrajPy between two distinguished trajectories is depicted. (C) and (F), (D), and (E) were adapted from Simões et al (2021) , Soares (2020) , and Mossman (2022), respectively.…”
Section: Methodsmentioning
confidence: 99%
“…Impaired mitochondrial biogenesis and mitochondrial dynamics [55][56][57] Excess ROS production [58] Decreased intracellular Ca 2+ buffering [58] Decreased respiratory capacity and/or loss of mitochondrial transmembrane potential [59] Disruption of intracellular trafficking-associated neurotoxicity [52,[60][61][62][63] mtDNA-mitochondrial DNA; OXPHOS-oxidative phosphorylation; TCA-tricarboxylic acid cycle; ROS-reactive oxygen species; AD-Alzheimer's disease, PD-Parkinson's disease; HD-Huntington's disease; ALS-amyotrophic lateral sclerosis.…”
Section: Redox-or Metabolic-related Alterations Referencesmentioning
confidence: 99%
“…Neurodegenerative diseases also have an important component of mitochondrial dysfunction and loss of redox homeostasis, as described in Table 1 [55][56][57][58][59][60]64]. Thus, mitochondria are important drug targets for neurodegenerative diseases, and some small molecules or peptide sequences targeting mitochondria are being developed (see Section 4.4.2) for different mitochondrial targets, since several mitochondrial structures/functions are affected in these diseases [65].…”
Section: Mitochondrial Dysfunctionmentioning
confidence: 99%