Quantitative Analysis of Nuclear Population in Muscle from Malnourished and Recovered Children

Abstract

Summarybution of nuclei among the cell types in muscle of children before and after recovery from severe PEM. Muscle tissue obtained by needle biopsy from the quadriceps femoris of eight malnourished and eight recovered children were examined histologically. In both groups about two-thirds of the MATERIALS AND METHODStotal nuclear count was due to muscle nuclei. The remaining Muscle samples were obtained by percutaneous needle biopsy one-third was made Up of vascular, nerve, and interstitial cells (7) of the quadriceps fernoris. Biopsies from eight malnourished and isolated nuclei in similar proportions in the two groups. A Jamaican children were obtained 2-7 days after their admission significantly greater percentage of muscle nuclei consisted of for severe PEM. The mean age (kSD) of these children on myogenic cells in recovered children as compared to malnour-admission was 15.9 (k4.8) months. Biopsies were taken from ished children (20.7 + 6.4 vs. 2.6 1.4%) eight other children who had recovered from sFvere PEM and had achieved, on average, 95% of their expected weight for Speculation height (50th percentile of the Boston standards). Their mean ages (kSD) on admission and after recovery were 16.0 (k3.2) The presence of a higher percentage of m~ogenic in and 18.5 (k3.2) months, respectively. The anthropometric muscle children who have P~~~~~~-~~~~~Y measurements of the malnourished children who were biopsied malnutrition suggests that muscle repletion involves hyperplastic and of the recovered group when they were admitted for PEM growth and that muscle maturation in recovered were similar

Wasting and Stunting—Similarities and Differences: Policy and Programmatic Implications

“…These measurements also indicate that the relationship between creatinine and 3-methyl histidine excretion is altered in children with SAM, suggesting an abnormal muscle tissue composition. This is consistent with major histological changes in muscle tissue observed in malnourished children, with a decreased proportion of myogenic cells compared with vascular, nerve, and interstitial cells [33].…”

Ultrastructure and Activity of Some Enzymes of Energy Metabolism of Skeletal Muscle in Experimental Energy Deficiency

“…Similar ultrastructural alterations have been re ported earlier [16,31,34], The disorganisa tion in the internal architecture of fibers in the localized areas is taken to be a nonspe cific indicator of damage, and has been re ported in normal human subjects [26]. In the present study, in addition to the findings described above, there were varied mito chondrial alterations.…”

Comparison of the chemical and biochemical composition of thirteen muscles of the rat after dietary protein restriction

“…This assumption has been tested for controls (Enesco & Puddy, 1964) but not for malnourished animals. This percentage of myonuclei has, however, also been found in muscle from malnourished infants (Hansen-Smith, Picou & Golden, 1978) and well-nourished children (F. Hansen-Smith, unpublished results). Direct counts of the total numbers of myonuclei in the flexor digiti brevis of pigs have shown neither loss nor gain in total myonuclei after I year of malnutrition (Stickland et al 1975).…”