Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC–ESI–TOF–MS

Abstract

Polyporus umbellatus is a well-known and important medicinal fungus in Asia. Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects. A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization–ion trap–time of flight–mass spectrometry. The hydrolysis conditions of the polysaccharides were optimized by orthogonal design. The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C. The resulting monosaccharides derivatized with 1-phenyl-3-methyl-5-pyrazolone have been well separated and analyzed by the established method. Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides. The results showed that polysaccharides in Polyporus umbellatus were composed of mannose, glucosamine, rhamnose, ribose, lyxose, erythrose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and fucose. Quantitative recoveries of these monosaccharides in the samples were in the range of 96.10–103.70%. This method is simple, accurate, and sensitive for the identification and quantification of monosaccharides, and can be applied to the quality control of Polyporusumbellatus as a natural medicine.

Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from Panax Ginseng C. A. Meyer

“…The monosaccharide composition analysis was performed by HPLC using the 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization method, as previously described [45]. Briefly, 10 mg of the sample was hydrolyzed in 10 mL of 1 M hydrochloric acid in methanol at 80 • C for 16 h under a nitrogen gas flow.…”

Compound Analysis of Jing Liqueur and nrf2 Activation by Jing Liqueur—One of the Most Popular Beverages in China

“…Iron (GB 5009, 90-2016), zinc (GB 5009, 14-2017), calcium (GB 5009, 92-2016), and amino acids (GB 5009, 124-2016) were analyzed according to the methods as described in the National Food Safety Standards, People's Republic of China. The concentration of total polysaccharides was measured according to the methods as published in the literatures [18]. Tables 1-3 for retention times of the 189 pure compounds and high performance liquid chromatography (HPLC) conditions including columns, flow-rates, and solvent systems).…”

“…Next, we screened almost all the 189 isolated molecules except twenty one (21) because of their insufficiency. Eighteen (18) compounds showed Nrf2 activation when compared with the negative control ( Figure 5). Among these eighteen Nrf2 activators (Figures 1, 2, and 5), four (55, 78, 129, and 168) from fraction IV, and the other fourteen (50, 51, 53, 58, 125, 126, 127, 128, 143, 144, 145, 146, 171, and 186) from fraction V (Table 4).…”

“…Analysis of Metals, Amino Acids, and Total Polysaccharides Iron (GB 5009, 90-2016), zinc (GB 5009, 14-2017), calcium (GB 5009, 92-2016), and amino acids (GB 5009, 124-2016) were analyzed according to the methods as described in the National Food Safety Standards, People's Republic of China. The concentration of total polysaccharides was measured according to the methods as published in the literatures[18].…”

Combined whole cell wall analysis and streamlined in silico carbohydrate-active enzyme discovery to improve biocatalytic conversion of agricultural crop residues

“…NMR and other MS techniques (e.g., MALDI-tof-MS) are complimentary to LC-ESI-MS/MS for structural analysis of oligosaccharides released enzymatically or chemically from plant residues [67,68,80]. Recently, there has been interest in the development of LC-MS-based methods for glyosidic linkage analysis [81][82][83][84][85], and LC-ESI-MS/MS methods have been developed for fast monosaccharide analysis with high sensitivity [86][87][88]. These novel methylation-LC-MS analyses are fast and sensitive and can be used to complement current GC-based linkage analyses.…”