2022
DOI: 10.1016/j.redox.2022.102343
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Quantitative analysis of redox proteome reveals oxidation-sensitive protein thiols acting in fundamental processes of developmental hematopoiesis

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Cited by 11 publications
(11 citation statements)
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“…Several proteomic approaches have been developed to examine cysteine availability, which can indicate its oxidation status, but many of these approaches require specialized reagents that are not commercially available [ 45 , 63 ]. We sought to characterize the redox proteome of Drosophila tissues with approaches that could be performed using readily available commercial reagents, leading us to focus on SICyLIA and iodo-TMT [ 53 , 64 ]. To identify which of these approaches would work most effectively with Drosophila samples, we compared these techniques in cultured Schneider 2 (S2) cells.…”
Section: Resultsmentioning
confidence: 99%
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“…Several proteomic approaches have been developed to examine cysteine availability, which can indicate its oxidation status, but many of these approaches require specialized reagents that are not commercially available [ 45 , 63 ]. We sought to characterize the redox proteome of Drosophila tissues with approaches that could be performed using readily available commercial reagents, leading us to focus on SICyLIA and iodo-TMT [ 53 , 64 ]. To identify which of these approaches would work most effectively with Drosophila samples, we compared these techniques in cultured Schneider 2 (S2) cells.…”
Section: Resultsmentioning
confidence: 99%
“…Several studies have examined redox proteomic changes in Drosophila tissues [ 56 , 57 ] and other organisms [ 45 , [48] , [49] , [50] , [51] , [52] , [53] , 64 ], but to our knowledge, this is the first study to characterize the redox proteome of either Drosophila S2 cells or the fly eye. Tissue-specific studies for redox proteomics remain relatively rare and can provide valuable insight into those proteins and pathways that are susceptible to oxidative modifications in particular cell types or tissues.…”
Section: Discussionmentioning
confidence: 99%
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