Quantitative analysis of simple lipid classes by thin-layer chromatography

Skipski, Vladimir P.; Good, James J.; Barclay, Marion; Reggio, Robert B.

doi:10.1016/0005-2760(68)90003-9

Cited by 170 publications

(64 citation statements)

References 28 publications

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“…Membrane lipids were extracted in isopropanol-chloroform and total lipid phosphorus determined (23). Individual phospholipids were isolated using thin layer chromatography on Silica Gel H plates obtained from Brinkmarin Instruments Incorporated, Westbury, NY (25). Phospholipids were scraped from each plate, eluted in isopropanol chloroform, and used for further lipid analysis.…”

Section: Methodsmentioning

confidence: 99%

Aspirin-induced Hemolysis: The Role of Concomitant Oxidant (H2O2) Challenge

1978

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Section: Methodsmentioning

confidence: 99%

Aspirin-induced Hemolysis: The Role of Concomitant Oxidant (H2O2) Challenge

1978

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“…The unsaponifiable lipids and the fatty acids were isolated as described previously (Kluytmans & Zandee, 1973). Aliquots of the total lipids were separated on thin-layer plates (0"25 mm silica gel DO) as described by Skipsky et al (1968).…”

Section: Methodsmentioning

confidence: 99%

Lipid metabolism in the northern pike (Esox LuciusL.)—3. In vivo incorporation of 1-14C-acetate in the lipids

Kluytmans

Zandee

1974

Comparative Biochemistry and Physiology Part B: Comparative Bio

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Abstract--1. The specific radioactivity of the total lipids isolated from different organs of the northern pike (Esox lucius L.) after injection of Na-1-14C-acetate showed the highest values in the liver and the gills.2. In the liver, radioactivity was found mainly in the fatty acids, while in the gills the greater part was present in the unsaponifiable lipids.3. The percentage incorporation of radioactivity in lipid classes did not change significantly from 2 to 24 hr after injection, but decreased in phospholipids and increased in triglycerides during shorter incubation times.4. The number of fatty acids labeled as well as the amount of radioactivity incorporated increased rapidly after 1-1*C-acetate injection. Virtually all fatty acids were labeled after 2 hr injection.

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“…The aqueous extraction phases were washed once with an equal volume of chloroform to remove residual lipid, and aqueous and total lipid radioactivity was counted (see below). Total lipid extracts were separated into neutral lipid subclasses by TLC on silica gel 60 plates (EM Separation Technologies; Gibbstown, NJ) using heptane-diethyl etherglacial acetic acid (60:40:3; v/v/v) (35). Authentic standards of triacylglycerol, phospholipids, cholesterol, cholesteryl ester, and unesterified fatty acids were run on the plates to identify the lipids.…”

Section: Separation and Analysis Of Stable Heart Lipidsmentioning

confidence: 99%

Rat heart cannot synthesize docosahexaenoic acid from circulating α-linolenic acid because it lacks elongase-2

Igarashi

Chang

et al. 2008

Journal of Lipid Research

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The extent to which the heart can convert alinolenic acid (a-LNA, 18:3n-3) to longer chain n-3 PUFAs is not known. Conversion rates can be measured in vivo using radiolabeled a-LNA and a kinetic fatty acid model. [1-14 C]a-LNA was infused intravenously for 5 min in unanesthetized rats that had been fed an n-3 PUFA-adequate [4.6% a-LNA, no docosahexaenoic acid (DHA, 22:6n-3)] or n-3 PUFA-deficient diet (0.2% a-LNA, nor DHA) for 15 weeks after weaning. Arterial plasma was sampled, as was the heart after high-energy microwaving. Rates of conversion of a-LNA to longer chain n-3 PUFAs were low, and DHA was not synthesized at all in the heart. Most a-LNA within the heart had been b-oxidized. In deprived compared with adequate rats, DHA concentrations in plasma and heart were both reduced by .90%, whereas heart and plasma levels of docosapentaenoic acid (DPAn-6, 22:5n-6) were elevated. Dietary deprivation did not affect cardiac mRNA levels of elongase-5 or desaturases D6 and D5, but elongase-2 mRNA could not be detected. In summary, the rat heart does not synthesize DHA from a-LNA, owing to the absence of elongase-2, but must obtain its DHA entirely from plasma. Dietary n-3 PUFA deprivation markedly reduces heart DHA and increases heart DPAn-6, which may make the heart vulnerable to different insults.-Igarashi, M., K. Ma, L. Chang, J. M. Bell, and S. I. Rapoport. Rat heart cannot synthesize docosahexaenoic acid from circulating a-linolenic acid because it lacks elongase-2. J. Lipid Res. 2008Res. . 49: 1735Res. -1745 Supplementary key words diet • heart • deprivation • elongation • synthesis • n-3 polyunsaturated fatty acids Long-chain n-3 PUFAs, particularly eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA,, are reported to be protective against cardiovascular disease (1-8). Thus, a low dietary n-3 PUFA intake and a low plasma DHA concentration are risk factors for cardiac disease (8-10), whereas a high dietary n-3 PUFA content is considered protective (11-13).In some mammalian tissues, EPA and DHA can be converted from shorter chain a-linolenic acid (a-LNA, 18:3n-3), which is enriched in plant oils, by serial steps of desaturation, elongation, and b-oxidation (14-20). Using our kinetic method and model, we showed in unanesthetized rats that rates of conversion of a-LNA to DHA were higher in liver than in brain, and that n-3 dietary deprivation could further increase the liver but not brain rates, in relation to elevated expression of requisite desaturases and elongases (21-23). These enzymes include D5 and D6 desaturases and elongases-2 and -5. They are expressed in the liver and many other rodent tissues, although elongase-2 has not been identified in rat heart (15-17, 24, 25). Thus, the heart may be incapable of synthesizing DHA from a-LNA, consistent with one study on isolated rat cardiomyocytes (26).Because of the importance of n-3 PUFAs to cardiovascular function (see above), and because their kinetics have not been thoroughly examined in vivo, we thought it of interest in this study...

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Quantitative analysis of simple lipid classes by thin-layer chromatography

Cited by 170 publications

References 28 publications

Aspirin-induced Hemolysis: The Role of Concomitant Oxidant (H2O2) Challenge

Aspirin-induced Hemolysis: The Role of Concomitant Oxidant (H2O2) Challenge

Lipid metabolism in the northern pike (Esox LuciusL.)—3. In vivo incorporation of 1-14C-acetate in the lipids

Rat heart cannot synthesize docosahexaenoic acid from circulating α-linolenic acid because it lacks elongase-2

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